公开(公告)号：US20110266225A1

公开(公告)日：2011-11-03

申请号：US13158780

申请日：2011-06-13

申请人： BO-LENNART JOHANSSON ,  JEAN-LUC MALOISEL ,  HANS J. JOHANSSON ,  ASA EK ,  MARIA HOLM ,  JAFAR SAIDZADEH

发明人： BO-LENNART JOHANSSON ,  JEAN-LUC MALOISEL ,  HANS J. JOHANSSON ,  ASA EK ,  MARIA HOLM ,  JAFAR SAIDZADEH

IPC分类号： B01D15/08 ,  B23P17/04

CPC分类号： B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065 ,  Y10T29/49826

摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

摘要翻译： 本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。

公开(公告)号：US20100130354A1

公开(公告)日：2010-05-27

申请号：US12692671

申请日：2010-01-25

申请人： JAN BERGSTROM ,  GUNNAR GLAD ,  BO-LENNART JOHANSSON ,  ANDERS LJUNGLOF ,  JEAN-LUC MALOISEL

发明人： JAN BERGSTROM ,  GUNNAR GLAD ,  BO-LENNART JOHANSSON ,  ANDERS LJUNGLOF ,  JEAN-LUC MALOISEL

IPC分类号： B01J20/22 ,  B01J20/291

CPC分类号： B01J20/3285 ,  B01D15/364 ,  B01J20/286 ,  B01J20/3244

摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.

摘要翻译： 本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。 在最有利的实施方案中，化学梯度是配体密度梯度。 本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。

公开(公告)号：US20100151581A1

公开(公告)日：2010-06-17

申请号：US12709556

申请日：2010-02-22

申请人： GUNNAR GLAD ,  BO-LENNART JOHANSSON ,  JEAN-LUC MALOISEL ,  NILS NORRMAN

发明人： GUNNAR GLAD ,  BO-LENNART JOHANSSON ,  JEAN-LUC MALOISEL ,  NILS NORRMAN

IPC分类号： G01N33/53 ,  B32B5/18 ,  C07K1/14 ,  C07K1/16

CPC分类号： B01J20/262 ,  B01J20/267 ,  B01J20/28019 ,  B01J20/28023 ,  B01J20/28033 ,  B01J20/28042 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3092 ,  B01J20/3208 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3246 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3255 ,  B01J20/3272 ,  B01J20/3285 ,  B01J2220/54 ,  B01J2220/58 ,  C07K16/065 ,  Y10T428/249963

摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.

摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一种脂族磺酰胺。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂肪族磺酰胺配体的分离基质上分离免疫球蛋白样化合物的方法。