公开(公告)号：US20110097464A1

公开(公告)日：2011-04-28

申请号：US12909120

申请日：2010-10-21

申请人： MARIA HOLM ,  OLA LIND ,  JAMES VAN ALSTINE

发明人： MARIA HOLM ,  OLA LIND ,  JAMES VAN ALSTINE

IPC分类号： C12H1/04 ,  C12G1/00 ,  A23L2/80

CPC分类号： A23L2/80 ,  A23L2/74 ,  C12H1/0424

摘要： The present invention relates to liquid clarification and stabilization. More closely, the invention relates to both clarification by removal of suspended particles, as well as stabilization against formation of non-microbial haze via reduction of haze forming substances in various liquids. The haze forming substances are proteins and polyphenol tannins which occur in various plant related fluids such as beer wine, juices, flavorings, plant extracts, and even bioprocess streams. The method of the invention accomplishes both size based removal of colloidal non-haze related particles as well as adsorption based removal of haze forming substances without a need for added flocculants. The to method of the invention utilizes hydrophilic surfaces for adsorption of haze forming substances with such surfaces presented by materials arranged in manner so that size based exclusion of suspended particles is also achieved.

摘要翻译： 本发明涉及液体澄清和稳定化。 更为详细地，本发明涉及通过除去悬浮颗粒进行澄清，以及通过减少各种液体中的雾状物质而形成非微生物雾度的稳定性。 烟雾形成物质是发生在各种植物相关液体中的蛋白质和多酚单宁，例如啤酒，果汁，调味剂，植物提取物，甚至生物过程流。 本发明的方法完成了胶体非雾度相关颗粒的基于尺寸的去除以及基于吸附的去除雾状形成物质而不需要添加的絮凝剂。 本发明的方法利用亲水表面吸附具有这种表面的雾状形成物质，所述表面由以下方式排列：材料排列方式使悬浮颗粒的尺寸排除也得以实现。

公开(公告)号：US20110266225A1

公开(公告)日：2011-11-03

申请号：US13158780

申请日：2011-06-13

申请人： BO-LENNART JOHANSSON ,  JEAN-LUC MALOISEL ,  HANS J. JOHANSSON ,  ASA EK ,  MARIA HOLM ,  JAFAR SAIDZADEH

发明人： BO-LENNART JOHANSSON ,  JEAN-LUC MALOISEL ,  HANS J. JOHANSSON ,  ASA EK ,  MARIA HOLM ,  JAFAR SAIDZADEH

IPC分类号： B01D15/08 ,  B23P17/04

CPC分类号： B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065 ,  Y10T29/49826

摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

摘要翻译： 本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。

公开(公告)号：US09266041B2

公开(公告)日：2016-02-23

申请号：US13158780

申请日：2011-06-13

申请人： Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Hans J. Johansson ,  Asa Ek ,  Maria Holm ,  Jafar Saidzadeh

发明人： Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Hans J. Johansson ,  Asa Ek ,  Maria Holm ,  Jafar Saidzadeh

IPC分类号： C07K1/14 ,  B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/28 ,  B01J20/286 ,  B01J20/32 ,  B01J41/20 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  C07K16/00 ,  C07K16/06

CPC分类号： B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065 ,  Y10T29/49826

摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

摘要翻译： 本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。