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    Separation Matrix And Method Of Purification
    7.
    发明申请
    Separation Matrix And Method Of Purification 有权
    分离矩阵和纯化方法

    公开(公告)号：US20070196858A1

    公开(公告)日：2007-08-23

    申请号：US11570530

    申请日：2005-06-22

    申请人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman

    发明人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman

    IPC分类号： G01N33/53 ,  C08G63/91 ,  C07K16/18

    CPC分类号： B01J20/3212 ,  B01D15/426 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251

    摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.

    摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。

    Adsorption method and ligands
    9.
    发明授权
    Adsorption method and ligands 有权
    吸附法和配体

    公开(公告)号：US07214321B2

    公开(公告)日：2007-05-08

    申请号：US10312054

    申请日：2001-07-16

    申请人： Makonnen Belew ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel

    发明人： Makonnen Belew ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel

    IPC分类号： B01D15/04

    CPC分类号： B01J20/3285 ,  B01J39/04 ,  B01J39/18

    摘要： The invention relates to a method for removing a positively charged substance from an aqueous liquid (I) by contacting the liquid with a cation-exchanger (1), possibly followed by a subsequent desorption of said substance. The cation-exchanger is selected to be capable of (a) binding to said substance by cation-exchange in an aqueous liquid reference (II) at an ionic strength corresponding to 0.3 M NaCl and (b) permitting a break through capacity for said substance 3200%, such as 3300% or 3500%, of the break-through capacity of said substance for a reference cation-exchanger (2) containing sulphopropyl groups —CH2CH2CH2SO2O—. The cation exchange ligands have an at least bimodal function by comprising a cation exchanging group and a separate hydrogen-bonding atom. The invention also relates to a method for testing the appropriateness of a cation-exchanger for removing a substance from a liquid and novel cation exchangers.

    摘要翻译： 本发明涉及通过使液体与阳离子交换剂（1）接触，从而可以随后随后解吸所述物质从水性液体（I）中除去带正电荷的物质的方法。 阳离子交换剂被选择为能够（a）在水性液体参考物（II）中以对应于0.3M NaCl的离子强度的阳离子交换结合所述物质，和（b）允许所述物质的破裂能力 例如300％或3％以上500％以上的所述物质用于参考阳离子交换器的穿透能力的200％ （2）含有磺基丙基-CH 2 CH 2 CH 2 SO 2 O-。 阳离子交换配体通过包含阳离子交换基团和单独的氢键原子具有至少双峰功能。 本发明还涉及一种用于测试用于从液体和新型阳离子交换剂中除去物质的阳离子交换器的适当性的方法。

    METHOD AND MEANS FOR SAMPLE PREPARATION
    10.
    发明申请
    METHOD AND MEANS FOR SAMPLE PREPARATION 审中-公开
    样品制备方法与手段

    公开(公告)号：US20140017813A1

    公开(公告)日：2014-01-16

    申请号：US14008001

    申请日：2012-03-29

    申请人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel

    发明人： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel

    IPC分类号： G01N1/40

    CPC分类号： G01N1/405 ,  B01D15/34 ,  B01D15/3866 ,  B01D15/3885 ,  B01J20/28009 ,  B01J20/285 ,  B01J20/3204 ,  B01J20/3227 ,  B01J20/3274 ,  B01J20/3293 ,  G01N30/14 ,  G01N2030/143

    摘要： The present invention relates to a method for depletion of undesired molecules and/or enrichment of desired molecules from a sample comprising high abundant as well as low abundant molecules, comprising the following steps: a) providing a separation material comprising a solid phase (beads) comprising an inner porous core material comprising magnetic particles and an outer porous shell with a porosity equal or denser than that of the shell; b) adding the sample to the separation material; c) adsorbing a first fraction of molecules with a molecular weight of 500-50 000 Da in the core and simultaneously excluding a second fraction of molecules from binding to the core and the shell, wherein the molecular weight of the second fraction molecules is at least 5 preferably 10 times higher than the molecular weight of the first fraction and d) eluting the desired molecules from the separation material, wherein step d) and optionally step c) is performed using an oscillating power/field applied over the separation material.The first fraction of molecules are for example drugs with a mw of about 700 Da, small proteins/peptides with an mw of about 7000 Da or proteins with a mw of about 40 000 Da.

    摘要翻译： 本发明涉及从包含高丰度和低丰度分子的样品中消耗不想要的分子和/或富集所需分子的方法，包括以下步骤：a）提供包含固相（珠） 包括包含磁性颗粒的内部多孔芯材料和具有等于或比密封件的孔隙度更高的孔隙的外部多孔壳体; b）将样品加入到分离材料中; c）在核心中吸附分子量为500-500Da的第一级分子，同时排除第二级分子与核心和壳结合，其中第二级分子的分子量至少为 5，优选比第一级分分子量高10倍; d）从分离材料洗脱所需的分子，其中步骤d）和任选的步骤c）使用施加在分离材料上的振荡功率/场进行。 分子的第一部分是例如mw为约700Da的药物，具有约7000Da的mw的小蛋白质/肽或约40,000Da的蛋白质。