公开(公告)号：US20150064760A1

公开(公告)日：2015-03-05

申请号：US14479071

申请日：2014-09-05

申请人： BRASKEM S.A.

发明人： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

IPC分类号： C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12N15/63

CPC分类号： C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

摘要： The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of crotonyl alcohol, 5-hydroxy-3-ketovaleryl-CoA, 3-ketopent-4-enoyl-CoA, or 3,5-ketovaleryl-CoA to butadiene; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of butadiene and 1-propanol and/or 1,2-propanediol.

摘要翻译： 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 在催化巴豆醇，5-羟基-3-酮戊酰基-CoA，3-酮戊-4-烯酰基-CoA或3,5-酮戊酰基-CoA转化为丁二烯的途径中编码一种或多种酶的一种或多种多核苷酸 ; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产丁二烯和1-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。

公开(公告)号：US20150064759A1

公开(公告)日：2015-03-05

申请号：US14478737

申请日：2014-09-05

申请人： Braskem S.A.

发明人： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

IPC分类号： C12P7/18 ,  C12P7/04

CPC分类号： C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

摘要： The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of cytosolic acetyl-CoA to 2-propanol; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of 2-propanol and 1-propanol and/or 1,2-propanediol.

摘要翻译： 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 一种或多种编码催化细胞溶质乙酰辅酶A转化为2-丙醇的途径中的一种或多种酶的多核苷酸; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产2-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。