公开(公告)号：US20150064760A1

公开(公告)日：2015-03-05

申请号：US14479071

申请日：2014-09-05

Applicant: BRASKEM S.A.

Inventor： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

IPC: C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12N15/63

CPC classification number: C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of crotonyl alcohol, 5-hydroxy-3-ketovaleryl-CoA, 3-ketopent-4-enoyl-CoA, or 3,5-ketovaleryl-CoA to butadiene; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of butadiene and 1-propanol and/or 1,2-propanediol.

Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 在催化巴豆醇，5-羟基-3-酮戊酰基-CoA，3-酮戊-4-烯酰基-CoA或3,5-酮戊酰基-CoA转化为丁二烯的途径中编码一种或多种酶的一种或多种多核苷酸 ; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产丁二烯和1-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。

公开(公告)号：US12163008B2

公开(公告)日：2024-12-10

申请号：US17333317

申请日：2021-05-28

Applicant: Braskem S.A.

Inventor： Felipe Cicaroni Fernandes ,  Iuri Estrada Gouvea ,  Antonio Rodolfo, Jr. ,  Mateus Schreiner Garcez Lopes

IPC: C07D307/68 ,  C08J3/12 ,  C08J3/18 ,  C08K5/00 ,  C08K5/1535 ,  C08L27/06

Abstract: The present disclosure generally relates to a compound of dialkyl ester of 2,4-furandicarboxylic acid, a method of preparing the compound, a polymer composition comprising a polymer and the compound, a method of preparing the polymer composition, a polymer product comprising the polymer composition and a method of using the compound as a plasticizer in the polymer product. The dialkyl ester of 2,4-furandicarboxylic acid of the present disclosure has greater plasticizing efficiency in a polymer composition that that of the standard phthalate and terephthalate-based plasticizers. The polymer product plasticized with the dialkyl ester of 2,4-furandicarboxylic acid may have improved flexibility, durability, processability and safety as compared to the same polymer product plasticized with conventional phthalate and terephthalate-based plasticizers.

公开(公告)号：US12037609B2

公开(公告)日：2024-07-16

申请号：US18084888

申请日：2022-12-20

Applicant: Braskem S.A.

Inventor： Verônica Leite Queiroz ,  Lucas Pedersen Parizzi ,  Iuri Estrada Gouvea ,  Debora Noma Okamoto ,  Rafael Victório Carvalho Guido ,  Alessandro Silva Nascimento ,  Igor Polikarpov

IPC: C12N9/04 ,  C12N1/21 ,  C12N15/52

CPC classification number: C12N9/0006 ,  C12N15/52 ,  C12Y101/01001

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US12018119B2

公开(公告)日：2024-06-25

申请号：US17586535

申请日：2022-01-27

Applicant: Braskem S.A.

Inventor： Felipe Cicaroni Fernandes ,  Iuri Estrada Gouvea ,  Bárbara Mano

IPC: C08G63/40 ,  C08G63/183

CPC classification number: C08G63/183 ,  C08G63/40

Abstract: The present disclosure provides a copolyester including a dicarboxylic acid component A comprising a terephthalic acid residue or ester-forming derivative thereof, or a mixture thereof, and a 2,4-furandicarboxylic acid residue or ester-forming derivative thereof, or a mixture thereof, and a diol component B comprising an alkanediol residue having from 2 to 22 carbon atoms, wherein the dicarboxylic acid component A has a total molar content, and wherein the 2,4-furandicarboxylic acid residue or ester-forming derivative thereof, is present in an amount of from 0.1 to 10 mol %, with respect to the total molar content of the dicarboxylic acid component A. Inventive copolyesters have a slower crystallization rate, a higher gas barrier to CO2 and O2 and a higher ratio of 14C to 12C as measured by ASTM D6866 when compared to a comparable copolyester comprising isophthalic acid instead of 2,4-furandicarboxylic acid.

公开(公告)号：US12006523B2

公开(公告)日：2024-06-11

申请号：US17545616

申请日：2021-12-08

Applicant: Braskem S.A.

Inventor： Iuri Estrada Gouvea ,  Paulo Moises Raduan Alexandrino

IPC: C12P17/04 ,  C07D307/68 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N9/08 ,  C12N9/16 ,  C12N9/88

CPC classification number: C12P17/04 ,  C07D307/68 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0065 ,  C12N9/16 ,  C12N9/88 ,  C12Y101/01 ,  C12Y102/01 ,  C12Y101/01001 ,  C12Y101/03 ,  C12Y102/01003 ,  C12Y102/01004 ,  C12Y102/01005 ,  C12Y207/01 ,  C12Y301/03 ,  C12Y402/03

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US10000744B2

公开(公告)日：2018-06-19

申请号：US15023579

申请日：2014-09-23

Applicant: BRASKEM S.A. ,  Avram Michael Slovic

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N1/19 ,  C12N1/21 ,  C12N9/16 ,  C12N9/10 ,  C12P7/04

CPC classification number: C12N9/16 ,  C12N9/13 ,  C12P7/04 ,  C12Y301/02011

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

公开(公告)号：US11566270B2

公开(公告)日：2023-01-31

申请号：US17219792

申请日：2021-03-31

Applicant: Braskem S.A.

Inventor： Paulo Moises Raduan Alexandrino ,  Iuri Estrada Gouvea ,  Veronica Leite Queiroz ,  Felipe Cicaroni Fernandes ,  Bárbara Mano

IPC: C12P17/04 ,  C07D307/68 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C12N9/08 ,  C12N9/16

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US20180346936A1

公开(公告)日：2018-12-06

申请号：US16042594

申请日：2018-07-23

Applicant: Braskem S.A.

Inventor： Mateus Schreiner Garcez Lopes ,  Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Johana Rincones Perez ,  Lucas Pedersen Parizzi

IPC: C12P5/02 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P5/026 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y402/01127

Abstract: The present disclosure generally relates to methods of using microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene and products and processes derived therefrom.

公开(公告)号：US20180327727A1

公开(公告)日：2018-11-15

申请号：US16011041

申请日：2018-06-18

Applicant: Braskem S.A.

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N9/16 ,  C12P7/04 ,  C12N9/10

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10x higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

公开(公告)号：US20160032325A1

公开(公告)日：2016-02-04

申请号：US14885311

申请日：2015-10-16

Applicant: Braskem S.A.

Inventor： Mateus Schreiner Garcez Lopes ,  Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Johana Rincones Perez ,  Lucas Pedersen Parizzi

IPC: C12P5/02 ,  C12N9/88

CPC classification number: C12P5/026 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y402/01127

Abstract: The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.