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    MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING BUTADIENE AND 1-PROPANOL AND/OR 1,2-PROPANEDIOL
    2.
    发明申请
    MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING BUTADIENE AND 1-PROPANOL AND/OR 1,2-PROPANEDIOL 审中-公开
    改良的微生物及其生产丁二酸和1-丙醇和/或1,2-丙二醇的方法

    公开(公告)号：US20150064760A1

    公开(公告)日：2015-03-05

    申请号：US14479071

    申请日：2014-09-05

    Applicant: BRASKEM S.A.

    Inventor： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

    IPC: C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12N15/63

    CPC classification number: C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

    Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of crotonyl alcohol, 5-hydroxy-3-ketovaleryl-CoA, 3-ketopent-4-enoyl-CoA, or 3,5-ketovaleryl-CoA to butadiene; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of butadiene and 1-propanol and/or 1,2-propanediol.

    Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 在催化巴豆醇，5-羟基-3-酮戊酰基-CoA，3-酮戊-4-烯酰基-CoA或3,5-酮戊酰基-CoA转化为丁二烯的途径中编码一种或多种酶的一种或多种多核苷酸 ; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产丁二烯和1-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。

    Microorganisms and methods for the production of glycolic acid and glycine via reverse glyoxylate shunt
    3.
    发明授权
    Microorganisms and methods for the production of glycolic acid and glycine via reverse glyoxylate shunt 有权

    公开(公告)号：US11384369B2

    公开(公告)日：2022-07-12

    申请号：US16791556

    申请日：2020-02-14

    Applicant: Braskem S.A.

    Inventor： Daniel Johannes Koch ,  Felipe Galzerani ,  Paulo Moises Raduan Alexandrino

    IPC: C12N9/88 ,  C12N9/90 ,  C12N1/20 ,  C12P7/42 ,  C12P13/00 ,  C12N9/00 ,  C12P13/04

    Abstract: The present invention provides biochemical pathways, glyoxylate producing recombinant microorganisms, and methods for the production and yield improvement of glycolic acid and/or glycine via a reverse glyoxylate shunt. The reverse glyoxylate shunt comprises an enzyme that catalyzes the carboxylation of phosphoenol pyruvate (PEP) to oxaloacetate (OAA), or an enzyme that catalyzes the carboxylation of pyruvate to oxaloacetate (OAA) or an enzyme that catalyzes the carboxylation of pyruvate to malate or a combination of any of the previous reactions; an enzyme that catalyzes the conversion of malate to malyl-CoA; an enzyme that catalyzes the conversion of malyl-CoA to glyoxylate and acetyl-CoA; and optionally an enzyme that catalyzes the conversion of oxaloacetate (OAA) to malate. Glyoxylate is reduced to produce glycolate. Alternatively, glyoxylate is converted to glycine. The reverse glyoxylate shunt pathway of the present invention can be utilized synergistically with other glycolic acid and/or glycine producing pathways to increase product yield.

    ENGINEERED ENZYME HAVING ACETOACETYL-COA HYDROLASE ACTIVITY, MICROORGANISMS COMPRISING SAME, AND METHODS OF USING SAME
    9.
    发明申请
    ENGINEERED ENZYME HAVING ACETOACETYL-COA HYDROLASE ACTIVITY, MICROORGANISMS COMPRISING SAME, AND METHODS OF USING SAME 有权
    具有乙酰胆碱-CA水解酶活性的工程化酶，包含其的微生物及其使用方法

    公开(公告)号：US20160208228A1

    公开(公告)日：2016-07-21

    申请号：US15023579

    申请日：2014-09-23

    Applicant: Avram Michael SLOVIC ,  BRASKEM S.A.

    Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

    IPC: C12N9/16

    CPC classification number: C12N9/16 ,  C12N9/13 ,  C12P7/04 ,  C12Y301/02011

    Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10x higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

    Abstract translation: 本公开提供了能够介导乙酰乙酰辅酶A向乙酰乙酸酯转化的工程化酶，其与乙酰辅酶A不具有与乙酰辅酶A相同数量级的反应（例如，工程化酶具有特定的乙酰乙酰基 - CoA水解酶活性比其乙酰-CoA水解酶活性高至少10倍）。 另外，本公开提供包含本文公开的工程化酶的修饰的微生物及其使用方法。

    MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING 2-PROPANOL AND1-PROPANOL AND/OR 1,2-PROPANEDIOL
    10.
    发明申请
    MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING 2-PROPANOL AND1-PROPANOL AND/OR 1,2-PROPANEDIOL 审中-公开
    改性微生物及其用于生产2-丙醇和1-丙醇和/或1,2-丙二醇的方法

    公开(公告)号：US20150064759A1

    公开(公告)日：2015-03-05

    申请号：US14478737

    申请日：2014-09-05

    Applicant: Braskem S.A.

    Inventor： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

    IPC: C12P7/18 ,  C12P7/04

    CPC classification number: C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

    Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of cytosolic acetyl-CoA to 2-propanol; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of 2-propanol and 1-propanol and/or 1,2-propanediol.

    Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 一种或多种编码催化细胞溶质乙酰辅酶A转化为2-丙醇的途径中的一种或多种酶的多核苷酸; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产2-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。

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