公开(公告)号：US5989907A

公开(公告)日：1999-11-23

申请号：US780836

申请日：1997-01-10

申请人： Barbara D. Boyan ,  Simon Van Dijk ,  David D. Dean

发明人： Barbara D. Boyan ,  Simon Van Dijk ,  David D. Dean

IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K38/00 ,  A61K39/00 ,  A61K39/395 ,  A61K49/00 ,  A61P1/00 ,  A61P1/02 ,  C07K14/34 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/31 ,  G01N33/53 ,  C12N15/85 ,  C07H21/02 ,  C07H21/04

CPC分类号： C07K14/34 ,  A61K38/00 ,  A61K39/00

摘要： The present invention relates to the cloning and sequencing of DNA encoding membrane-associated proteolipids from C. matruchotii to vectors comprising the DNA and to host cells containing vectors including the novelty DNA.

摘要翻译： 本发明涉及将编码膜相关蛋白脂蛋白的DNA从棉铃虫到包含DNA的载体和含有载体的宿主细胞的克隆和测序，所述载体包括新颖DNA。

公开(公告)号：US06444446B1

公开(公告)日：2002-09-03

申请号：US09448192

申请日：1999-11-23

申请人： Barbara D. Boyan ,  Simon Van Dijk ,  David D. Dean

发明人： Barbara D. Boyan ,  Simon Van Dijk ,  David D. Dean

IPC分类号： C12N1509

CPC分类号： C07K14/34 ,  A61K38/00 ,  A61K39/00

摘要： The invention relates to the cloning and sequencing of the DNA encoding a membrane associated proteolipid from C. matruchotii that is capable of inducing in vitro calcium binding. A proteolipid was extracted from cultures of C. matruchotii and separated into a lipid component and three apoproteins having molecular weights of approximately 5.0, 5.5 and 7.5 kDa as determined by SDS-PAGE. The invention also includes the polyclonal and monoclonal antibodies directed against the membrane associated proteolipid and immunological assays developed with these antibodies.

摘要翻译： 本发明涉及编码能够诱导体外钙结合的来自马鞭毛霉的膜相关蛋白脂质的DNA的克隆和测序。 从马鞭毛虫的培养物中提取蛋白脂质，并通过SDS-PAGE测定分离出分子量约为5.0,5.5和7.5kDa的脂质组分和三种脱辅基蛋白。 本发明还包括针对膜相关蛋白脂质的多克隆和单克隆抗体以及用这些抗体开发的免疫测定。

公开(公告)号：US6079864A

公开(公告)日：2000-06-27

申请号：US146686

申请日：1998-09-03

申请人： David D. Dean

发明人： David D. Dean

IPC分类号： B01F5/12 ,  B01F3/04 ,  B01D45/00

CPC分类号： B01F5/12

摘要： A fluid mixing system including a liquid mixing device and method for generating and maintaining a substantially uniform, horizontal velocity profile in a liquid circulation system, such as a plug flow reactor, which requires less horsepower than conventional liquid mixing systems without losing mixing effectiveness. The liquid mixing device employs a top-entry, vertically-oriented impeller and a housing that directs the pumped flow in a generally horizontal direction. The liquid mixing module may be positioned in close proximity to an aeration source within a channel.

摘要翻译： 一种流体混合系统，包括液体混合装置和用于在液体循环系统（例如活塞式流动反应器）中产生和保持基本上均匀的水平速度分布的方法，该流体混合装置与常规液体混合系统相比需要较少的功率而不失去混合效果。 液体混合装置采用顶部进入的垂直取向的叶轮和将泵送流体大致水平的方向引导的壳体。 液体混合模块可以定位成靠近通道内的曝气源。

公开(公告)号：US5803601A

公开(公告)日：1998-09-08

申请号：US726680

申请日：1996-10-07

申请人： David D. Dean

发明人： David D. Dean

IPC分类号： B01F5/12 ,  B01F7/22

CPC分类号： B01F5/12

摘要： A fluid mixing system including a fluid mixing device and method for generating and maintaining a substantially uniform, horizontal velocity profile in a fluid circulation system, such as a plug flow reactor, which requires less horsepower than conventional fluid mixing systems without losing mixing effectiveness. The fluid mixing device employs a top-entry, vertically-oriented impeller and a housing that directs the pumped flow in a generally horizontal direction.

摘要翻译： 一种流体混合系统，包括流体混合装置和用于在流体循环系统（例如活塞流反应器）中产生和维持基本上均匀的水平速度分布的方法，其需要比常规流体混合系统更少的功率而不失去混合效果。 流体混合装置采用顶部进入的垂直取向的叶轮和将泵送流体大致水平的方向引导的壳体。