公开(公告)号：US10125348B2

公开(公告)日：2018-11-13

申请号：US14991263

申请日：2016-01-08

Applicant: Battelle Memorial Institute

Inventor： Alex S. Beliaev ,  Ryan S. McClure ,  Hans C. Bernstein ,  Stephen R. Lindemann ,  G. Chris Jansson

IPC: C12N1/20 ,  C12Q1/68 ,  C12Q1/6874 ,  C12N1/00 ,  C12N1/38 ,  C12N15/115 ,  C12N15/63

Abstract: Self-sustained, safe, stable and scalable microbial consortia (S5MicroCon) are described. The microbial consortia are regulated by photoautotroph-heterotroph interactions and RNA aptamer-based gene circuits. A rapid, high-throughput method for engineering RNA aptamer-based gene circuits (e.g. riboswitches) is also described.

公开(公告)号：US20160201026A1

公开(公告)日：2016-07-14

申请号：US14991263

申请日：2016-01-08

Applicant: Battelle Memorial Institute

Inventor： Alex S. Beliaev ,  Ryan S. McClure ,  Hans C. Bernstein ,  Stephen R. Lindemann ,  G. Chris Jansson

IPC: C12N1/20 ,  C12Q1/68

CPC classification number: C12N1/20 ,  C07K14/195 ,  C07K14/245 ,  C12N1/00 ,  C12N1/38 ,  C12N15/1093 ,  C12N15/115 ,  C12N15/635 ,  C12N15/70 ,  C12N15/74 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q1/6874

Abstract: Self-sustained, safe, stable and scalable microbial consortia (S5MicroCon) are described. The microbial consortia are regulated by photoautotroph-heterotroph interactions and RNA aptamer-based gene circuits. A rapid, high-throughput method for engineering RNA aptamer-based gene circuits (e.g. riboswitches) is also described.

Abstract translation: 描述了自我维持，安全，稳定和可扩展的微生物联盟（S5MicroCon）。 微生物联合体由光自养 - 异养相互作用和基于RNA适体的基因电路调节。 还描述了用于工程化基于RNA适体的基因电路（例如核糖开关）的快速，高通量的方法。

公开(公告)号：US10731202B2

公开(公告)日：2020-08-04

申请号：US15527996

申请日：2015-11-11

Applicant: Battelle Memorial Institute

Inventor： Stephen R. Lindemann ,  Aaron T. Wright

IPC: C12Q1/6809 ,  C12N15/10 ,  C12Q1/6816 ,  C12Q1/6869

Abstract: Methods and systems for identifying binding sites in macromolecules using small molecule mimics of naturally occurring molecules is disclosed. A reactive probe is provided that mimics small molecule cofactors. A target macromolecule is irreversibly bound to the probe in vivo to selectively pull down or precipitate probe-bound macromolecules. The macromolecules may be, but are not limited to, DNA, RNA, and proteins.

公开(公告)号：US20170342469A1

公开(公告)日：2017-11-30

申请号：US15527996

申请日：2015-11-11

Applicant: Battelle Memorial Institute

Inventor： Stephen R. Lindemann ,  Aaron T. Wright

IPC: C12Q1/68

Abstract: Methods and systems for identifying binding sites in macromolecules using small molecule mimics of naturally occurring molecules is disclosed. A reactive probe is provided that mimics small molecule cofactors. A target macromolecule is irreversibly bound to the probe in vivo to selectively pull down or precipitate probe-bound macromolecules. The macromolecules may be, but are not limited to, DNA, RNA, and proteins.