公开(公告)号：US10113967B2

公开(公告)日：2018-10-30

申请号：US15209640

申请日：2016-07-13

Applicant: Becton, Dickinson and Company

Inventor： Pierce O. Norton ,  Yong Qin Chen

IPC: G01N21/64 ,  G01N15/14 ,  G01N15/10 ,  G01N15/00

Abstract: The present invention provides systems and methods for analyzing the excitation spectra of fluorescent particles in a flowing stream. The system uses a white light laser and color separation optics to provide a spatially-distributed, continuous color-spectrum excitation light system that is used to illuminate a region of a flowing stream. A particle that passes through the detection region traverses the full dispersed spectrum of excitation light, and the fluorescence emissions from the particle are continuously measured as it passes through the detection region. The measured fluorescence emissions at each wavelength of excitation light, which changes through full spectrum of the excitation light as the particle passes through the detection region, provides the excitation spectrum of the particle.

公开(公告)号：US20160320304A1

公开(公告)日：2016-11-03

申请号：US15209640

申请日：2016-07-13

Applicant: Becton, Dickinson and Company

Inventor： Pierce O. Norton ,  Yong Qin Chen

IPC: G01N21/64 ,  G01N15/14

CPC classification number: G01N21/6428 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N21/64 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/144 ,  G01N2015/1477 ,  G01N2021/6417 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/0633 ,  G01N2201/068

Abstract: The present invention provides systems and methods for analyzing the excitation spectra of fluorescent particles in a flowing stream. The system uses a white light laser and color separation optics to provide a spatially-distributed, continuous color-spectrum excitation light system that is used to illuminate a region of a flowing stream. A particle that passes through the detection region traverses the full dispersed spectrum of excitation light, and the fluorescence emissions from the particle are continuously measured as it passes through the detection region. The measured fluorescence emissions at each wavelength of excitation light, which changes through full spectrum of the excitation light as the particle passes through the detection region, provides the excitation spectrum of the particle.

Abstract translation: 本发明提供了用于分析流动流中的荧光颗粒的激发光谱的系统和方法。 该系统使用白光激光器和分色光学器件来提供用于照亮流动区域的空间分布的连续色谱激发光系统。 通过检测区域的粒子穿过激发光的全部分散光谱，并且当粒子通过检测区域时，粒子的荧光发射被连续地测量。 在颗粒通过检测区域时，通过激发光的全谱变化的激发光的每个波长处的测量的荧光发射提供了颗粒的激发光谱。