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公开(公告)号:US20230079640A1
公开(公告)日:2023-03-16
申请号:US17931217
申请日:2022-09-12
Applicant: Berkeley Lights, Inc.
Inventor: Duane Smith , Jason M. McEwen
IPC: C12Q1/6806 , C12Q1/6865 , C12Q1/6876
Abstract: The disclosure provides methods for amplification of paired transcript sequences from a single cell, such as, but not limited to alpha and beta T cell receptor (TCR) sequences from a single T cell. The method can include: placing a single T cell into a cell lysis solution to provide a T cell lysate comprising RNA; generating first strand cDNA from the RNA; amplifying the first strand cDNA to provide amplified cDNA; and amplifying the alpha and beta TCR sequences from the amplified cDNA in a single reaction, wherein the alpha and beta TCR sequences are amplified using three sets of TCR amplification primers. In some instances, the methods can be performed using no more than six sets of primers.
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公开(公告)号:US20210087252A1
公开(公告)日:2021-03-25
申请号:US16987835
申请日:2020-08-07
Inventor: Hideho Okada , Duane Smith , Payal Watchmaker , Yelena Bronevetsky , Ryosuke Naka , Guido K. Stadler , Xiaohua Wang , Kevin T. Chapman
IPC: C07K14/725 , C12N5/0783 , C12N15/63
Abstract: This disclosure relates to the production and use of an isolated, purified and/or recombinant T cell receptor (TCR) that specifically binds to a mutant IDH1 protein, or a fragment thereof, wherein the mutant IDH1 protein or fragment thereof comprises an R132H mutation.
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公开(公告)号:US20200299351A1
公开(公告)日:2020-09-24
申请号:US16743849
申请日:2020-01-15
Applicant: Berkeley Lights, Inc.
Inventor: Peter J. Beemiller , Alexander J. Mastroianni , Shao Ning Pei , Randall D. Lowe, JR. , Annamaria Mocciaro , Kevin D. Loutherback , Yelena Bronevetsky , Guido K. Stadler , Andrew W. McFarland , Kevin T. Chapman , Duane Smith , Natalie C. Marks , Amanda L. Goodsell
IPC: C07K14/725 , C07K16/28 , G01N33/50 , C07K14/74
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.
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