-
公开(公告)号：US20160312165A1
公开(公告)日：2016-10-27
申请号：US15135707
申请日：2016-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, JR. , Kristin Beaumont , Aathavan Karunakaran , Natalie Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
Abstract translation: 描述了用于在微流体装置中培养一个或多个生物细胞的系统，方法和试剂盒，包括提供配置成增强细胞生长，活力，可移植性或其任何组合的营养物和气体组分。 在一些实施方案中，培养单细胞可以在微流体装置中产生克隆群体。
-
公开(公告)号：US20180099275A1
公开(公告)日：2018-04-12
申请号：US15785727
申请日：2017-10-17
Inventor： Ming-Chiang Wu , Jodi Tsu-An Loo , Shao Ning Pei , Gaetan L. Mathieu , Jian Gong , Randall D. Lowe, JR. , Justin K. Valley
IPC: B01L3/00 , G01N27/447
Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.
-
公开(公告)号：US20210368781A1
公开(公告)日：2021-12-02
申请号：US17228058
申请日：2021-04-12
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, JR.
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
-
公开(公告)号：US20200064337A1
公开(公告)日：2020-02-27
申请号：US16391063
申请日：2019-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Minha Park , Jason C. Briggs , Jason M. McEwen , Ravi K. Ramenani , Hariharasudhan Chirra Dinakar , Kai W. Szeto , Adrienne T. Higa , Mark P. White , Randall D. Lowe, JR. , Xiaohua Wang , Kevin T. Chapman
IPC: G01N33/50 , B01L3/00 , C12N5/0781 , G01N33/68 , C12Q1/6876
Abstract: Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.
-
公开(公告)号：US20210291171A1
公开(公告)日：2021-09-23
申请号：US17162467
申请日：2021-01-29
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, JR. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC: B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
-
Patent Agency Ranking
公开(公告)号：US20160338347A1
公开(公告)日：2016-11-24
申请号：US15136777
申请日：2016-04-22
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, JR.
CPC classification number: A01N1/0284 , A01N1/0221 , A01N1/0263 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/021 , B01L2300/024 , B01L2300/0864 , B01L2300/16 , B01L2300/163 , B01L2300/18 , B01L2300/1894 , C12M47/04
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
Abstract translation: 一种处理和存储生物细胞的方法包括将可流动介质引入微流体装置，所述可流动介质包括生物细胞; 在微流体装置的一个或多个隔离区域中从可流动介质中隔离一个或多个生物细胞; 以及冷冻微流体装置，其中包含一个或多个生物细胞。
-
公开(公告)号：US20230182136A1
公开(公告)日：2023-06-15
申请号：US18164124
申请日：2023-02-03
Applicant: BERKELEY LIGHTS, INC.
Inventor： Kristin G. Beaumont , Non-Linda Ding , Volker L.S. Kurz , Troy A. Lionberger , Randall D. Lowe, JR. , Daniele Malleo , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
IPC: B01L3/00 , B01J19/00 , G01N27/447
CPC classification number: B01L3/502753 , B01L3/502761 , B01L3/502738 , B01J19/0093 , B01L3/5023 , B01L3/502707 , G01N27/44791 , B01L2400/0424 , B01L2300/0816 , B01L2300/0864 , B01L2400/0677 , B01L2200/0668 , B01L2300/16 , B01L2400/08
Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.
-
8.发明申请公开(公告)号：US20200299351A1
公开(公告)日：2020-09-24
申请号：US16743849
申请日：2020-01-15
Applicant: Berkeley Lights, Inc.
Inventor： Peter J. Beemiller , Alexander J. Mastroianni , Shao Ning Pei , Randall D. Lowe, JR. , Annamaria Mocciaro , Kevin D. Loutherback , Yelena Bronevetsky , Guido K. Stadler , Andrew W. McFarland , Kevin T. Chapman , Duane Smith , Natalie C. Marks , Amanda L. Goodsell
IPC: C07K14/725 , C07K16/28 , G01N33/50 , C07K14/74
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.
-
公开(公告)号：US20190275516A1
公开(公告)日：2019-09-12
申请号：US16196649
申请日：2018-11-20
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, JR. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
-
公开(公告)号：US20180135011A1
公开(公告)日：2018-05-17
申请号：US15802100
申请日：2017-11-02
Applicant: Berkeley Lights, Inc.
Inventor： Yelena Bronevetsky , Xiaohua Wang , Peter J. Beemiller , Kristin G. Beaumont , Randall D. Lowe, JR. , Alexander J. Mastroianni , Kevin T. Chapman
IPC: C12N5/0783 , A61P35/00 , C12M3/06 , A61K39/00 , C12M1/00
CPC classification number: C12N5/0636 , A61K35/17 , A61K39/0011 , A61K2035/124 , A61K2039/5158 , A61P35/00 , C12M23/16 , C12M29/10 , C12N2501/2302 , C12N2501/51 , C12N2501/515 , C12N2502/1121 , C12N2533/50
Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.
-
-
-
-
-
-
-
-
-
Search Results
12
records
(took 0.034 seconds)
