公开(公告)号：US11203018B2

公开(公告)日：2021-12-21

申请号：US16160816

申请日：2018-10-15

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. Lionberger ,  Matthew E. Fowler ,  Phillip J. M. Elms ,  Kevin D. Loutherback ,  Randall D. Lowe, Jr. ,  Jian Gong ,  J. Tanner Nevill ,  Gang F. Wang ,  Gregory G. Lavieu ,  John A. Tenney ,  Aathavan Karunakaran ,  Anupam Singhal ,  I-Jong Lin

IPC: B01L3/00 ,  G01N21/64 ,  B03C5/02 ,  B03C5/00 ,  B01L9/00 ,  G06T7/00 ,  G01N21/05

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20190283026A1

公开(公告)日：2019-09-19

申请号：US16253869

申请日：2019-01-22

Applicant: Berkeley Lights, Inc.

Inventor： Kevin D. Loutherback ,  Yelena Bronevetsky ,  Peter J. Beemiller ,  Xiaohua Wang ,  Kevin T. Chapman

IPC: B01L3/00 ,  G01N1/34 ,  A61K35/17

Abstract: Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

公开(公告)号：US11666912B2

公开(公告)日：2023-06-06

申请号：US16253869

申请日：2019-01-22

Applicant: Berkeley Lights, Inc.

Inventor： Kevin D. Loutherback ,  Yelena Bronevetsky ,  Peter J. Beemiller ,  Xiaohua Wang ,  Kevin T. Chapman

IPC: B01L3/00 ,  A61K35/17 ,  G01N1/34 ,  B03C5/02 ,  A61K35/28 ,  B03C5/00 ,  G01N1/40

CPC classification number: B01L3/502746 ,  A61K35/17 ,  A61K35/28 ,  B01L3/502761 ,  B01L3/502792 ,  B03C5/005 ,  B03C5/026 ,  G01N1/34 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C2201/26 ,  G01N2001/4088

Abstract: Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

公开(公告)号：US20200299351A1

公开(公告)日：2020-09-24

申请号：US16743849

申请日：2020-01-15

Applicant: Berkeley Lights, Inc.

Inventor： Peter J. Beemiller ,  Alexander J. Mastroianni ,  Shao Ning Pei ,  Randall D. Lowe, JR. ,  Annamaria Mocciaro ,  Kevin D. Loutherback ,  Yelena Bronevetsky ,  Guido K. Stadler ,  Andrew W. McFarland ,  Kevin T. Chapman ,  Duane Smith ,  Natalie C. Marks ,  Amanda L. Goodsell

IPC: C07K14/725 ,  C07K16/28 ,  G01N33/50 ,  C07K14/74

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.