公开(公告)号：US20190283026A1

公开(公告)日：2019-09-19

申请号：US16253869

申请日：2019-01-22

Applicant: Berkeley Lights, Inc.

Inventor： Kevin D. Loutherback ,  Yelena Bronevetsky ,  Peter J. Beemiller ,  Xiaohua Wang ,  Kevin T. Chapman

IPC: B01L3/00 ,  G01N1/34 ,  A61K35/17

Abstract: Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

公开(公告)号：US11273177B2

公开(公告)日：2022-03-15

申请号：US15488139

申请日：2017-04-14

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T Chapman ,  Xiaohua Wang ,  Xiao Guan Radstrom ,  Yelena Bronevetsky ,  Guido K Stadler ,  Gregory G Lavieu ,  Annamaria Mocciaro

IPC: A61K35/17 ,  C07K14/55 ,  C12N9/24 ,  C07K16/28 ,  B01L3/00 ,  C07K14/195 ,  C12N5/0783 ,  A61K31/65 ,  C12N5/00 ,  C12N5/0781 ,  C07K14/47 ,  C07K14/52 ,  A61K39/00

Abstract: The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.

公开(公告)号：US20210087252A1

公开(公告)日：2021-03-25

申请号：US16987835

申请日：2020-08-07

Applicant: Berkeley Lights, Inc. ,  The Regents of the University of California

Inventor： Hideho Okada ,  Duane Smith ,  Payal Watchmaker ,  Yelena Bronevetsky ,  Ryosuke Naka ,  Guido K. Stadler ,  Xiaohua Wang ,  Kevin T. Chapman

IPC: C07K14/725 ,  C12N5/0783 ,  C12N15/63

Abstract: This disclosure relates to the production and use of an isolated, purified and/or recombinant T cell receptor (TCR) that specifically binds to a mutant IDH1 protein, or a fragment thereof, wherein the mutant IDH1 protein or fragment thereof comprises an R132H mutation.

公开(公告)号：US20170224734A1

公开(公告)日：2017-08-10

申请号：US15488139

申请日：2017-04-14

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T Chapman ,  Xiaohua Wang ,  Xiao Guan Radstrom ,  Yelena Bronevetsky ,  Guido K Stadler ,  Gregory G Levieu ,  Annamaria Mocciaro

IPC: A61K35/17 ,  C07K14/55 ,  C12N9/24 ,  C07K16/28 ,  B01L3/00 ,  C07K14/195 ,  C12N5/0783 ,  A61K31/65

CPC classification number: A61K35/17 ,  A61K31/65 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0861 ,  B01L2300/0896 ,  B01L2400/0424 ,  C07K14/195 ,  C07K14/4748 ,  C07K14/52 ,  C07K14/55 ,  C07K16/2818 ,  C07K2317/14 ,  C07K2319/00 ,  C12N5/00 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0637 ,  C12N5/0638 ,  C12N9/2402 ,  C12N2501/04 ,  C12N2510/00 ,  C12Y302/01166

Abstract: The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.

公开(公告)号：US11666912B2

公开(公告)日：2023-06-06

申请号：US16253869

申请日：2019-01-22

Applicant: Berkeley Lights, Inc.

Inventor： Kevin D. Loutherback ,  Yelena Bronevetsky ,  Peter J. Beemiller ,  Xiaohua Wang ,  Kevin T. Chapman

IPC: B01L3/00 ,  A61K35/17 ,  G01N1/34 ,  B03C5/02 ,  A61K35/28 ,  B03C5/00 ,  G01N1/40

CPC classification number: B01L3/502746 ,  A61K35/17 ,  A61K35/28 ,  B01L3/502761 ,  B01L3/502792 ,  B03C5/005 ,  B03C5/026 ,  G01N1/34 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C2201/26 ,  G01N2001/4088

Abstract: Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

公开(公告)号：US20200299351A1

公开(公告)日：2020-09-24

申请号：US16743849

申请日：2020-01-15

Applicant: Berkeley Lights, Inc.

Inventor： Peter J. Beemiller ,  Alexander J. Mastroianni ,  Shao Ning Pei ,  Randall D. Lowe, JR. ,  Annamaria Mocciaro ,  Kevin D. Loutherback ,  Yelena Bronevetsky ,  Guido K. Stadler ,  Andrew W. McFarland ,  Kevin T. Chapman ,  Duane Smith ,  Natalie C. Marks ,  Amanda L. Goodsell

IPC: C07K14/725 ,  C07K16/28 ,  G01N33/50 ,  C07K14/74

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.

公开(公告)号：US20180135011A1

公开(公告)日：2018-05-17

申请号：US15802100

申请日：2017-11-02

Applicant: Berkeley Lights, Inc.

Inventor： Yelena Bronevetsky ,  Xiaohua Wang ,  Peter J. Beemiller ,  Kristin G. Beaumont ,  Randall D. Lowe, JR. ,  Alexander J. Mastroianni ,  Kevin T. Chapman

IPC: C12N5/0783 ,  A61P35/00 ,  C12M3/06 ,  A61K39/00 ,  C12M1/00

CPC classification number: C12N5/0636 ,  A61K35/17 ,  A61K39/0011 ,  A61K2035/124 ,  A61K2039/5158 ,  A61P35/00 ,  C12M23/16 ,  C12M29/10 ,  C12N2501/2302 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2502/1121 ,  C12N2533/50

Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.