公开(公告)号：US20170173580A1

公开(公告)日：2017-06-22

申请号：US15336768

申请日：2016-10-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Shao Ning PEI ,  Jian GONG ,  Alexander J. MASTROIANNI ,  Jason M. MCEWEN ,  Justin K. VALLEY

IPC: B01L3/00

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section or module having a dielectrophoresis configuration; systems that include any of the microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the medium of the aqueous droplet; related kits; and methods of manipulating droplets, optionally containing micro-objects such as biological cells, within the microfluidic devices.

公开(公告)号：US20210114020A1

公开(公告)日：2021-04-22

申请号：US17005116

申请日：2020-08-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, Jr. ,  Shao Ning PEI ,  Jian GONG ,  Alexander J. MASTROIANNI ,  Jason M. MCEWEN ,  Justin K. VALLEY

IPC: B01L3/00 ,  C12M1/00 ,  C12M3/06

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section having a dielectrophoresis configuration; systems that include microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the aqueous droplet; related kits; and methods of manipulating droplets within the microfluidic devices.