公开(公告)号：US20220356429A1

公开(公告)日：2022-11-10

申请号：US17743318

申请日：2022-05-12

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Kristin G. BEAUMONT ,  Aathavan KARUNAKARAN ,  Natalie C. MARKS ,  Jason M. MCEWEN ,  Mark P. WHITE ,  J. Tanner NEVILL ,  Gang F. WANG ,  Andrew W. MCFARLAND ,  Daniele Malleo ,  Keith J. BREINLINGER ,  Xiao GUAN ,  Kevin T. CHAPMAN

IPC: C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20210071124A1

公开(公告)日：2021-03-11

申请号：US16920365

申请日：2020-07-02

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Kristin G. BEAUMONT ,  Aathavan KARUNAKARAN ,  Natalie C. MARKS ,  Jason M. MCEWEN ,  Mark P. WHITE ,  J. Tanner NEVILL ,  Gang F. WANG ,  Andrew W. MCFARLAND ,  Daniele Malleo ,  Keith J. BREINLINGER ,  Xiao GUAN ,  Kevin T. CHAPMAN

IPC: C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20210349075A1

公开(公告)日：2021-11-11

申请号：US17241865

申请日：2021-04-27

Applicant: BERKELEY LIGHTS, INC.

Inventor： Yelena BRONEVETSKY ,  Annamaria MOCCIARO ,  Guido K. STADLER ,  Peter J. BEEMILLER ,  Natalie C. MARKS ,  Duane SMITH ,  Vincent Haw Tien PAI ,  Jason M. MCEWEN ,  Amanda L. GOODSELL ,  John A. TENNEY ,  Thomas M. VETTERLI ,  Hansohl E. Kim

IPC: G01N33/50 ,  B01L3/00

Abstract: Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

公开(公告)号：US20170173580A1

公开(公告)日：2017-06-22

申请号：US15336768

申请日：2016-10-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Shao Ning PEI ,  Jian GONG ,  Alexander J. MASTROIANNI ,  Jason M. MCEWEN ,  Justin K. VALLEY

IPC: B01L3/00

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section or module having a dielectrophoresis configuration; systems that include any of the microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the medium of the aqueous droplet; related kits; and methods of manipulating droplets, optionally containing micro-objects such as biological cells, within the microfluidic devices.

公开(公告)号：US20230201827A1

公开(公告)日：2023-06-29

申请号：US18167638

申请日：2023-02-10

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. KURZ ,  Jason M. MCEWEN ,  Kellen C. MOBILIA ,  Alexander J. MASTROIANNI ,  Joshua J. CARDIEL RIVERA

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/087 ,  B01L2300/0877

Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.

公开(公告)号：US20220161255A1

公开(公告)日：2022-05-26

申请号：US17452725

申请日：2021-10-28

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. KURZ ,  Jason M. MCEWEN ,  Kellen C. MOBILIA ,  Alexander J. MASTROIANNI ,  Joshua J. CARDIEL RIVERA

IPC: B01L3/00

Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.

公开(公告)号：US20190060907A1

公开(公告)日：2019-02-28

申请号：US16024624

申请日：2018-06-29

Applicant: BERKELEY LIGHTS, INC.

Inventor： X. Robert BAO ,  Jason M. MCEWEN ,  Brian A. RABKIN

IPC: B01L3/00 ,  G01N35/10

CPC classification number: B01L3/502792 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2400/0427 ,  B01L2400/0496 ,  G01N35/10 ,  G01N2035/00237 ,  G01N2035/1034

Abstract: Systems and methods are described herein for improved droplet generation within microfluidic apparatuses. Electrowetting forces of varying configurations may be used to separate droplets from a fluidic reservoir in a reproducible and rapid manner. In many embodiments, separation of droplets from the fluidic reservoir is performed without the use of highly specialized surfactants.

公开(公告)号：US20210114020A1

公开(公告)日：2021-04-22

申请号：US17005116

申请日：2020-08-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, Jr. ,  Shao Ning PEI ,  Jian GONG ,  Alexander J. MASTROIANNI ,  Jason M. MCEWEN ,  Justin K. VALLEY

IPC: B01L3/00 ,  C12M1/00 ,  C12M3/06

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section having a dielectrophoresis configuration; systems that include microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the aqueous droplet; related kits; and methods of manipulating droplets within the microfluidic devices.