公开(公告)号：US20170173580A1

公开(公告)日：2017-06-22

申请号：US15336768

申请日：2016-10-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Shao Ning PEI ,  Jian GONG ,  Alexander J. MASTROIANNI ,  Jason M. MCEWEN ,  Justin K. VALLEY

IPC: B01L3/00

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section or module having a dielectrophoresis configuration; systems that include any of the microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the medium of the aqueous droplet; related kits; and methods of manipulating droplets, optionally containing micro-objects such as biological cells, within the microfluidic devices.

公开(公告)号：US20220033758A1

公开(公告)日：2022-02-03

申请号：US17401783

申请日：2021-08-13

Applicant: BERKELEY LIGHTS, INC.

Inventor： Eric K. SACKMANN ,  Nathan J. VER HEUL ,  Ryan M. COOPER ,  Alexander J. MASTROIANNI ,  Annamaria MOCCIARO ,  Vincent Haw Tien PAI ,  Randall D. LOWE, JR. ,  Hector D. NEIRA-QUINTERO ,  Patrick N. INGRAM ,  Smriti SRIDHAR

IPC: C12M1/34 ,  C12M1/00 ,  C12M3/06 ,  C12N1/04

Abstract: Methods for laser-assisted repositioning of a micro-object and for culturing an attachment-dependent biological cell within a microfluidic device are described herein. Laser illumination is used to controllably create a bubble which repositions the micro-object. Further, methods of culturing an attachment-dependent biological cell are described, where the methods may include laser-assisted repositioning.

公开(公告)号：US20210071124A1

公开(公告)日：2021-03-11

申请号：US16920365

申请日：2020-07-02

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Kristin G. BEAUMONT ,  Aathavan KARUNAKARAN ,  Natalie C. MARKS ,  Jason M. MCEWEN ,  Mark P. WHITE ,  J. Tanner NEVILL ,  Gang F. WANG ,  Andrew W. MCFARLAND ,  Daniele Malleo ,  Keith J. BREINLINGER ,  Xiao GUAN ,  Kevin T. CHAPMAN

IPC: C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20220356429A1

公开(公告)日：2022-11-10

申请号：US17743318

申请日：2022-05-12

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Kristin G. BEAUMONT ,  Aathavan KARUNAKARAN ,  Natalie C. MARKS ,  Jason M. MCEWEN ,  Mark P. WHITE ,  J. Tanner NEVILL ,  Gang F. WANG ,  Andrew W. MCFARLAND ,  Daniele Malleo ,  Keith J. BREINLINGER ,  Xiao GUAN ,  Kevin T. CHAPMAN

IPC: C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20220250071A1

公开(公告)日：2022-08-11

申请号：US17534350

申请日：2021-11-23

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. LIONBERGER ,  Matthew E. FOWLER ,  Phillip J. M. ELMS ,  Kevin D. LOUTHERBACK ,  Randall D. LOWE, JR. ,  Jian GONG ,  J. Tanner NEVILL ,  Gang F. WANG ,  Gregory G. LAVIEU ,  John A. TENNEY ,  Aathavan KARUNAKARAN ,  Anupam SINGHAL ,  I-Jong LIN

IPC: B01L3/00 ,  G01N21/64 ,  B03C5/02 ,  B03C5/00 ,  B01L9/00 ,  G06T7/00

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20190240665A1

公开(公告)日：2019-08-08

申请号：US16160816

申请日：2018-10-15

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. LIONBERGER ,  Matthew E. FOWLER ,  Phillip J. M. ELMS ,  Kevin D. LOUTHERBACK ,  Randall D. LOWE, JR. ,  Jian GONG ,  Tanner J. NEVILL ,  Gang F. WANG ,  Gregory G. LAVIEU ,  John A. TENNEY ,  Aathavan KARUNAKARAN

IPC: B01L3/00 ,  G06T7/00 ,  B01L9/00

CPC classification number: B01L3/502761 ,  B01L9/527 ,  B01L2300/0829 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2201/0675 ,  G06T7/0014

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.