公开(公告)号：US20230251268A1

公开(公告)日：2023-08-10

申请号：US17930712

申请日：2022-09-08

Applicant: BERKELEY LIGHTS, INC.

Inventor： Phillip J.M. ELMS ,  Michelle SORKIN ,  Kevin D. LOUTHERBACK ,  Randall D. LOWE, Jr.

IPC: G01N33/68 ,  B01L3/00

CPC classification number: G01N33/6854 ,  B01L3/502715 ,  B01L2400/0424 ,  B01L2200/0647 ,  B01L2400/0472 ,  B01L2300/0654

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20210114020A1

公开(公告)日：2021-04-22

申请号：US17005116

申请日：2020-08-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, Jr. ,  Shao Ning PEI ,  Jian GONG ,  Alexander J. MASTROIANNI ,  Jason M. MCEWEN ,  Justin K. VALLEY

IPC: B01L3/00 ,  C12M1/00 ,  C12M3/06

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section having a dielectrophoresis configuration; systems that include microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the aqueous droplet; related kits; and methods of manipulating droplets within the microfluidic devices.