公开(公告)号：US20220033758A1

公开(公告)日：2022-02-03

申请号：US17401783

申请日：2021-08-13

Applicant: BERKELEY LIGHTS, INC.

Inventor： Eric K. SACKMANN ,  Nathan J. VER HEUL ,  Ryan M. COOPER ,  Alexander J. MASTROIANNI ,  Annamaria MOCCIARO ,  Vincent Haw Tien PAI ,  Randall D. LOWE, JR. ,  Hector D. NEIRA-QUINTERO ,  Patrick N. INGRAM ,  Smriti SRIDHAR

IPC: C12M1/34 ,  C12M1/00 ,  C12M3/06 ,  C12N1/04

Abstract: Methods for laser-assisted repositioning of a micro-object and for culturing an attachment-dependent biological cell within a microfluidic device are described herein. Laser illumination is used to controllably create a bubble which repositions the micro-object. Further, methods of culturing an attachment-dependent biological cell are described, where the methods may include laser-assisted repositioning.

公开(公告)号：US20170173580A1

公开(公告)日：2017-06-22

申请号：US15336768

申请日：2016-10-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Shao Ning PEI ,  Jian GONG ,  Alexander J. MASTROIANNI ,  Jason M. MCEWEN ,  Justin K. VALLEY

IPC: B01L3/00

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section or module having a dielectrophoresis configuration; systems that include any of the microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the medium of the aqueous droplet; related kits; and methods of manipulating droplets, optionally containing micro-objects such as biological cells, within the microfluidic devices.

公开(公告)号：US20230201827A1

公开(公告)日：2023-06-29

申请号：US18167638

申请日：2023-02-10

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. KURZ ,  Jason M. MCEWEN ,  Kellen C. MOBILIA ,  Alexander J. MASTROIANNI ,  Joshua J. CARDIEL RIVERA

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/087 ,  B01L2300/0877

Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.

公开(公告)号：US20220161255A1

公开(公告)日：2022-05-26

申请号：US17452725

申请日：2021-10-28

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. KURZ ,  Jason M. MCEWEN ,  Kellen C. MOBILIA ,  Alexander J. MASTROIANNI ,  Joshua J. CARDIEL RIVERA

IPC: B01L3/00

Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.

公开(公告)号：US20210114020A1

公开(公告)日：2021-04-22

申请号：US17005116

申请日：2020-08-27

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, Jr. ,  Shao Ning PEI ,  Jian GONG ,  Alexander J. MASTROIANNI ,  Jason M. MCEWEN ,  Justin K. VALLEY

IPC: B01L3/00 ,  C12M1/00 ,  C12M3/06

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section having a dielectrophoresis configuration; systems that include microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the aqueous droplet; related kits; and methods of manipulating droplets within the microfluidic devices.