公开(公告)号：US20030162891A1

公开(公告)日：2003-08-28

申请号：US10087140

申请日：2002-02-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Cory M. Panattoni

IPC: C08K003/00 ,  C08L001/00

CPC classification number: G01N27/44747 ,  C08F220/56 ,  C08K5/17 ,  G01N27/44704 ,  Y10S524/916 ,  Y10S526/934 ,  C08L33/26

Abstract: Irregularities in the pore structures of polyacrylamide gels that are formed in cassettes against plastic walls are reduced or eliminated by the inclusion of an oxygen scavenger in the gel-forming solution. Avoidance of the irregularities results in electropherograms with fewer distortions in the solute bands.

Abstract translation: 通过在凝胶形成溶液中包含氧清除剂，减少或消除了在塑料壁上形成的聚丙烯酰胺凝胶的孔结构中的不规则性。 避免不规则性导致电泳图在溶质带中具有较少的失真。

公开(公告)号：US20040134784A1

公开(公告)日：2004-07-15

申请号：US10746453

申请日：2003-12-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Cory M. Panattoni ,  Lee Olech

IPC: C25B011/00

CPC classification number: G01N27/44704

Abstract: In pre-cast slab gel cassettes, the formation of pathways in which proteins can migrate between the gel and the walls of the cassette to form shadow bands is avoided by the application of a coating of a nonionic amphiphilic polymer to the cassette walls. The coating also prevents the gel from sticking to the walls when the gel is to be removed from the cassette after electrophoresis.

Abstract translation: 在预制板坯凝胶盒中，通过将非离子两亲聚合物的涂层施加到盒壁上，避免蛋白质可以在胶体与盒壁之间迁移以形成阴影带的途径的形成。 当凝胶在电泳后要从盒中取出时，涂层也可以防止凝胶粘到壁上。

公开(公告)号：US20040195102A1

公开(公告)日：2004-10-07

申请号：US10408835

申请日：2003-04-03

Applicant: BIO-RAD LABORATORIES, INC. a corporation of the state of Delaware

Inventor： Cory M. Panattoni ,  Donald E. Gueffroy ,  Roger S. Provost

IPC: G01N027/453

CPC classification number: G01N27/44747

Abstract: An electrophoresis gel is prepared with indicia incorporated into the gel itself rather than on a label or backing that is adhered to the gel. According to one method, the indicia are applied by first coating a solid surface that will be used as one internal surface of a gel mold with a solution of a water-permeable polymer, allowing the coating to dry, imprinting indicia in ink on the dry coating, and then forming the gel over the coating. In a variation on this method, the indicia are applied to a sheet which is then placed against the mold surface, the sheet being either a liner that is separable from the gel yet coated with the polymer or a sheet of the polymer itself. Alternatively, the indicia are printed directly on the mold surface without first coating the surface. This is achieved by using as a printing composition a mixture of an ink and a solution of a water-permeable polymer, then forming the gel over the imprinted indicia.

Abstract translation: 制备电泳凝胶，其标记掺入凝胶本身，而不是贴在凝胶上的标签或背衬上。 根据一种方法，通过首先涂覆将用作凝胶模具的一个内表面的固体表面与水可渗透聚合物溶液一起涂覆标记，使涂层干燥，在干燥的油墨中的印记标记 涂层，然后在涂层上形成凝胶。 在该方法的变型中，将标记施加到片材上，然后将其放置在模具表面上，片材是可与聚合物或聚合物本身片材涂布的凝胶分离的衬里。 或者，标记直接印在模具表面上，而不首先涂覆表面。 这通过使用油墨和水可渗透聚合物的溶液的混合物作为印刷组合物，然后在印迹标记上形成凝胶来实现。

公开(公告)号：US20040140214A1

公开(公告)日：2004-07-22

申请号：US10623480

申请日：2003-07-18

Applicant: BIO-RAD LABORATORIES, INC., a corporation of the state of Delaware

Inventor： Cory M. Panattoni

IPC: C07K001/26

CPC classification number: G01N27/44747 ,  C07K1/26

Abstract: In pre-cast slab gel cassettes, the formation of pathways in which proteins can migrate between the gel and the walls of the cassette to form shadow bands is avoided by including a nonionic amphiphilic polymer in the monomer solution from which the gel is formed and casting the gel with the polymer included. The nonionic amphiphilic polymer also prevents the resulting gel from sticking to the walls when the gel is to be removed from the cassette after electrophoresis.

Abstract translation: 在预制板坯凝胶盒中，通过在凝胶形成和铸造的单体溶液中包含非离子两亲性聚合物来避免蛋白质在凝胶和盒壁之间迁移以形成阴影带的途径的形成 包含聚合物的凝胶。 当凝胶在电泳后从盒中取出时，非离子两亲性聚合物还可防止所得凝胶粘附到壁上。

公开(公告)号：US20040140215A1

公开(公告)日：2004-07-22

申请号：US10346681

申请日：2003-01-17

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Cory M. Panattoni

IPC: G01L001/20 ,  B01D057/02 ,  C07K001/26

CPC classification number: C07K1/26 ,  G01N27/44747

Abstract: In pre-cast slab gel cassettes, the formation of pathways in which proteins can migrate between the gel and the walls of the cassette to form shadow bands is avoided by including a nonionic amphiphilic polymer in the monomer solution from which the gel is formed and casting the gel with the polymer included. The nonionic amphiphilic polymer also prevents the resulting gel from sticking to the walls when the gel is to be removed from the cassette after electrophoresis.

公开(公告)号：US20030168576A1

公开(公告)日：2003-09-11

申请号：US10095563

申请日：2002-03-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Cory M. Panattoni

IPC: B29C039/10

CPC classification number: G01N27/44795 ,  B29C33/12 ,  B29C33/68 ,  B29C39/10 ,  B29C39/26 ,  B29K2105/0061

Abstract: Isoelectric focusing gels are cast in a rod-shaped casting mold split longitudinally into two halves, each half having a half-circle profile so that the combined halves form a completed mold that is tubular in shape with a circular cross section. Flat grooves are cut into the flat contacting surfaces of each of the mold halves, the grooves in direct opposition to one another to form a chamber of rectangular cross section extending the full length of the mold and open at both ends. One groove receives a flexible backing strip for the gel and the other serves as the casting chamber for the gel. Once the gel is cast in this mold, isoelectric focusing can be performed as if the gel were a tube gel, and the two mold halves are then readily separated to permit removal of the gel and its transfer if desired to a second dimension separation.

公开(公告)号：US20020074229A1

公开(公告)日：2002-06-20

申请号：US09973179

申请日：2001-10-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Cory M. Panattoni ,  Lee Olech

IPC: G01N027/26

CPC classification number: G01N27/44704

Abstract: In pre-cast slab gel cassettes, the formation of pathways in which proteins can migrate between the gel and the walls of the cassette to form shadow bands is avoided by the application of a coating of a nonionic amphiphilic polymer to the cassette walls. The coating also prevents the gel from sticking to the walls when the gel is to be removed from the cassette after electrophoresis.

Abstract translation: 在预制板坯凝胶盒中，通过将非离子两亲聚合物的涂层施加到盒壁上，避免蛋白质可以在胶体与盒壁之间迁移以形成阴影带的途径的形成。 当凝胶在电泳后要从盒中取出时，涂层也可以防止凝胶粘到壁上。