公开(公告)号：US12201974B2

公开(公告)日：2025-01-21

申请号：US16984852

申请日：2020-08-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yochanan Uri ,  Itay Barak ,  Robert Lovchik ,  David P. Taylor ,  Marcel Buerge ,  Govind V. Kaigala

IPC: B01L3/00

Abstract: The present disclosure is notably directed to a microfluidic probe head (202), or MFP head, comprising a processing surface (204) having liquid injection and liquid aspiration apertures, as well as projections (205) extending from the processing surface (204). The arrangement of injection and aspiration apertures provides for a hydrodynamic flow confinement within a processing region that is formed between the processing surface (204) and a substrate (104) or sample surface (for example, the bottom of a microtiter plate sample well (102)), typically located beneath the processing surface (204). The disclosure is further directed to related microfluidic probe devices, and methods of operation of such an MFP head, notably to deposit cells on a surface.

公开(公告)号：US12011714B2

公开(公告)日：2024-06-18

申请号：US16984955

申请日：2020-08-04

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Emilie Frisan ,  Laurent Guillon ,  Robert Lovchik ,  David P. Taylor ,  Claudia I. Trainito ,  Govind V. Kaigala

IPC: B01L3/00 ,  B01L3/02

CPC classification number: B01L3/502707 ,  B01L3/0293 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2400/0487

Abstract: The present disclosure is notably directed to a microfluidic probe head, or MFP head, comprising a processing surface having a liquid injection aperture and a liquid aspiration aperture thereon. The aspiration aperture is generally shaped so as to partly extend around the injection aperture on the processing surface, although such injection apertures are not completely surrounded by the slit on the processing surface. Further, fluidic and solid barriers to aspiration are considered. The disclosure is further directed to related microfluidic probe devices, and methods of operation of such an MFP head, notably to deposit cells on a surface.