公开(公告)号：US10048256B2

公开(公告)日：2018-08-14

申请号：US15073518

申请日：2016-03-17

Applicant: Bio-Rad Laboratories, Inc

Inventor： Jan zur Megede

IPC: G01N33/543 ,  B01L3/00 ,  B01L3/02

Abstract: Sample analysis systems and methods are provided. In one embodiment, the method may be achieved by applying a substance to a surface of a substrate having a first binding agent immobilized thereon; removing unbound material from at least a portion of the substrate having the substance applied thereon; applying a second binding agent to the surface of the substrate, wherein the second binding agent is optically labeled or unlabeled; removing unbound material from at least a portion of the substrate having the second binding agent applied thereon; responsive to detecting the optically labeled second binding agent bound to the substance, identifying the analyte present in the sample; and responsive to not detecting the optically labeled second binding agent bound to the substance, determining that the analyte is absent in the sample; wherein the applying the substance or second binding agent to the surface of the substrate steps are concurrent with the respective removing unbound material from at least a portion of the substrate steps. Systems and other methods are also described and illustrated.

公开(公告)号：US10809250B2

公开(公告)日：2020-10-20

申请号：US15752108

申请日：2016-08-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jan zur Megede ,  George Karlin-Neumann

IPC: G01N33/542 ,  G01N33/58

Abstract: Methods of detecting an analyte in a sample are provided. In one embodiment, the method comprises forming a partition comprising the sample, a binding agent capable of emitting a signal when bound to the analyte, and a marker capable of identifying the partition; allowing the binding agent to bind to the analyte, if present; and detecting the presence of the analyte in the sample by detecting the signal emitted from the binding agent in the partition.

公开(公告)号：US20160274097A1

公开(公告)日：2016-09-22

申请号：US15073518

申请日：2016-03-17

Applicant: Bio-Rad Laboratories, Inc,

Inventor： Jan zur Megede

IPC: G01N33/543

CPC classification number: G01N33/54306 ,  B01L3/0241 ,  B01L3/5027 ,  B01L3/5088 ,  B01L2300/0861

Abstract: Sample analysis systems and methods are provided. In one embodiment, the method may be achieved by applying a substance to a surface of a substrate having a first binding agent immobilized thereon; removing unbound material from at least a portion of the substrate having the substance applied thereon; applying a second binding agent to the surface of the substrate, wherein the second binding agent is optically labeled or unlabeled; removing unbound material from at least a portion of the substrate having the second binding agent applied thereon; responsive to detecting the optically labeled second binding agent bound to the substance, identifying the analyte present in the sample; and responsive to not detecting the optically labeled second binding agent bound to the substance, determining that the analyte is absent in the sample; wherein the applying the substance or second binding agent to the surface of the substrate steps are concurrent with the respective removing unbound material from at least a portion of the substrate steps. Systems and other methods are also described and illustrated.

Abstract translation: 提供样品分析系统和方法。 在一个实施方案中，该方法可以通过将物质施加到其上固定有第一结合剂的基底的表面上来实现; 从其上施加有物质的基材的至少一部分去除未结合的材料; 将第二粘合剂施加到所述基底的表面，其中所述第二粘合剂是光学标记的或未标记的; 从其上施加有第二粘合剂的基材的至少一部分去除未结合的材料; 响应于检测与物质结合的光学标记的第二结合剂，鉴定样品中存在的分析物; 并且响应于不检测与物质结合的光学标记的第二结合剂，确定样品中不存在分析物; 其中将所述物质或第二粘合剂施加到所述基底台阶的表面与从所述基底步骤的至少一部分分离的去除未结合的材料同时进行。 还描述和示出了系统和其他方法。