公开(公告)号：US20150353883A1

公开(公告)日：2015-12-10

申请号：US14760182

申请日：2014-01-10

Applicant: BIOGEN MA INC.

Inventor： Alan GILBERT ,  Kyle MCELEARNEY ,  Terrence Michael DOBROWSKY ,  Rashmi Rohit KSHIRSAGAR

IPC: C12N5/00 ,  C12P21/00 ,  C07K14/755

CPC classification number: C12N5/0018 ,  C07K14/755 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/34 ,  C12N2511/00 ,  C12P21/00

Abstract: The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.

Abstract translation: 本发明涉及包含硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物的细胞培养基及其使用方法。 本发明还涉及在大规模细胞培养物中生产感兴趣的蛋白质的方法，其包括用硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物补充细胞培养物。

公开(公告)号：US20170327786A1

公开(公告)日：2017-11-16

申请号：US15523009

申请日：2015-10-30

Applicant: Biogen MA Inc.

Inventor： Yao-Ming HUANG ,  William C. YANG ,  Alan GILBERT ,  Kyle MCELEARNEY

IPC: C12N5/00 ,  C07K16/28 ,  C07K14/565 ,  C07K14/705 ,  C07K16/24 ,  C07K16/18 ,  C07K16/42 ,  C07K14/525 ,  C12P21/00 ,  C07K16/00

CPC classification number: C12N5/0018 ,  C07K14/525 ,  C07K14/565 ,  C07K14/70575 ,  C07K16/00 ,  C07K16/18 ,  C07K16/241 ,  C07K16/246 ,  C07K16/2839 ,  C07K16/2887 ,  C07K16/42 ,  C07K2317/14 ,  C12N2500/33 ,  C12N2500/38 ,  C12N2501/845 ,  C12N2501/999 ,  C12N2510/02 ,  C12N2511/00 ,  C12P21/00

Abstract: The present invention pertains to a cell culture medium comprising hypotaurine, GABA, and/or beta-alanine or the combination of choline and hypotaurine, GABA, and/or beta-alanine as media supplements which is shown to control viability, growth, and waste byproduct accumulation. The present invention further pertains to a method of producing a polypeptide of interest in a large scale cell culture containing hypotaurine, GABA, and/or beta-alanine or the combination of choline and hypotaurine, GABA, and/or beta-alanine.