公开(公告)号：US20180171362A1

公开(公告)日：2018-06-21

申请号：US15578736

申请日：2016-06-01

Applicant: Biogen MA Inc.

Inventor： Jessica Mondia ,  Alan Gilbert ,  Maureen Lanan

IPC: C12P1/00 ,  C12Q1/37 ,  G01N33/533

CPC classification number: C12P1/00 ,  C12P21/02 ,  C12Q1/37 ,  C12Y304/21093 ,  C12Y304/21094 ,  G01N33/533 ,  G01N2333/95

Abstract: Some aspects of the present disclosure provide methods, compositions and kits for identifying one or more reagents, such as a culture medium, that are effective for recombinant protein production.

公开(公告)号：US20160131634A1

公开(公告)日：2016-05-12

申请号：US14892723

申请日：2014-05-29

Applicant: BIOGEN MA INC.

Inventor： Weiwei Hu ,  Haofan Peng ,  Erik Hughes ,  Kelly Wiltberger ,  Maureen Lanan ,  Amr Ali

IPC: G01N33/483

CPC classification number: G01N33/4833 ,  A61K47/10 ,  C12M27/02 ,  C12M41/32

Abstract: The present disclosure shows, unexpectedly, that variations in cell culture performance in large-scale cell culture systems such as, for example, those used in commercial manufacturing processes, in some instances, can be attributed to often subtle variations among shear-protectant additives used during cell culture. Assessing the quality of shear-protective additives using such large-scale systems, however, is inaccurate, time-consuming and costly. To solve the problem identified, the present disclosure provides methods and compositions for evaluating the suitability of shear-protectant additives without resorting to large scale cell growth and/or protein production tests.

Abstract translation: 本公开意外地显示，在一些情况下，大规模细胞培养系统（例如在商业制造过程中使用的那些）中的细胞培养性能的变化可以归因于所使用的剪切保护剂添加剂之间经常微妙的变化 在细胞培养过程中。 然而，使用这种大规模系统评估剪切保护添加剂的质量是不准确的，耗时且昂贵的。 为了解决所确定的问题，本公开提供了用于评估剪切保护剂添加剂的适用性而不诉诸大规模细胞生长和/或蛋白质生产测试的方法和组合物。