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1.发明申请公开(公告)号：US20180155753A1
公开(公告)日：2018-06-07
申请号：US15578847
申请日：2016-06-01
Applicant: Biogen MA Inc.
Inventor： Lia Tescione , Thomas Ryll , Alan Gilbert
CPC classification number: C12P21/005 , C07K16/2842 , C07K2317/14 , C07K2317/41 , C12N5/0018 , C12N5/0603 , C12N5/0682 , C12N2500/20 , C12N2511/00
Abstract: The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.
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公开(公告)号：US20210340584A1
公开(公告)日：2021-11-04
申请号：US17102361
申请日：2020-11-23
Applicant: Biogen MA Inc.
Inventor： Lia Tescione , Thomas Ryll , Alan Gilbert
Abstract: The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.
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公开(公告)号：US20180171362A1
公开(公告)日：2018-06-21
申请号：US15578736
申请日：2016-06-01
Applicant: Biogen MA Inc.
Inventor： Jessica Mondia , Alan Gilbert , Maureen Lanan
IPC: C12P1/00 , C12Q1/37 , G01N33/533
CPC classification number: C12P1/00 , C12P21/02 , C12Q1/37 , C12Y304/21093 , C12Y304/21094 , G01N33/533 , G01N2333/95
Abstract: Some aspects of the present disclosure provide methods, compositions and kits for identifying one or more reagents, such as a culture medium, that are effective for recombinant protein production.
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Patent Agency Ranking
公开(公告)号：US20170247655A1
公开(公告)日：2017-08-31
申请号：US15425056
申请日：2017-02-06
Applicant: BIOGEN MA INC.
Inventor： Rashmi Rohit KSHIRSAGAR , Alan Gilbert
IPC: C12N5/07 , A61K39/395 , C12M1/02
CPC classification number: C12P21/00 , A61K39/39591 , A61K2039/505 , C07K1/1133 , C07K16/00 , C07K2317/40 , C07K2317/41 , C07K2317/94 , C12N5/0018 , C12N2500/34
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
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公开(公告)号：US09790533B2
公开(公告)日：2017-10-17
申请号：US15425056
申请日：2017-02-06
Applicant: BIOGEN MA INC.
Inventor： Rashmi Rohit Kshirsagar , Alan Gilbert
CPC classification number: C12P21/00 , A61K39/39591 , A61K2039/505 , C07K1/1133 , C07K16/00 , C07K2317/40 , C07K2317/41 , C07K2317/94 , C12N5/0018 , C12N2500/34
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
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公开(公告)号：US20170029859A1
公开(公告)日：2017-02-02
申请号：US15291257
申请日：2016-10-12
Applicant: BIOGEN MA INC.
Inventor： Alan Gilbert , Kyle McElearney , Terrence Michael Dobrowsky , Rashmi Rohit Kshirsagar
IPC: C12P21/00 , C07K14/755 , C12N5/00
CPC classification number: C07K14/755 , C12N5/0018 , C12N2500/24 , C12N2500/34 , C12N2510/02 , C12N2511/00 , C12P21/02
Abstract: The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.
Abstract translation: 本发明涉及包含硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物的细胞培养基及其使用方法。 本发明还涉及在大规模细胞培养物中生产感兴趣的蛋白质的方法，其包括用硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物补充细胞培养物。
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公开(公告)号：US10590454B2
公开(公告)日：2020-03-17
申请号：US15785268
申请日：2017-10-16
Applicant: Biogen MA Inc.
Inventor： Rashmi Rohit Kshirsagar , Alan Gilbert
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
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公开(公告)号：US20180251805A1
公开(公告)日：2018-09-06
申请号：US15785268
申请日：2017-10-16
Applicant: Biogen MA Inc.
Inventor： Rashmi Rohit Kshirsagar , Alan Gilbert
IPC: C12P21/00 , C12N5/00 , A61K39/395 , C07K16/00 , C07K1/113
CPC classification number: C12P21/00 , A61K39/39591 , A61K2039/505 , C07K1/1133 , C07K16/00 , C07K2317/40 , C07K2317/41 , C07K2317/94 , C12N5/0018 , C12N2500/34
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
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