公开(公告)号：US20160289633A1

公开(公告)日：2016-10-06

申请号：US15038698

申请日：2014-12-19

Applicant: Biogen MA Inc.

Inventor： William C. YANG ,  Jiuyi LU ,  Yao-Ming HUANG ,  Hang YUAN ,  Rashmi KSHIRSAGAR ,  Thomas RYLL

IPC: C12N5/00

CPC classification number: C12N5/0018 ,  C07K16/00 ,  C07K2317/14 ,  C12N2500/10 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2511/00

Abstract: Methods of improving the efficiency of production of a protein product of interest in mammalian cell culture are presented. In particular, the methods result in an increase in the quantity of a protein product produced, or decreases protein product production time in a manufacturing-scale bioreactor cell culture. The disclosed methods comprise: (a) culturing the N-1 bioreactor culture to high viable cell densities; and (b) seeding the production bioreactor culture at high viable cell seeding densities.

Abstract translation: 提出了提高哺乳动物细胞培养中感兴趣的蛋白质产品生产效率的方法。 特别地，所述方法导致产生的蛋白质产物的量的增加，或减少制造规模的生物反应器细胞培养物中的蛋白质产物生产时间。 所公开的方法包括：（a）将N-1生物反应器培养物培养成高活细胞密度; 和（b）将生产生物反应器培养物以高活细胞播种密度接种。

公开(公告)号：US20170175075A1

公开(公告)日：2017-06-22

申请号：US15128732

申请日：2015-03-24

Applicant: BIOGEN MA Inc. ,  SAMSUNG BIOEPIS

Inventor： William YANG ,  Yao-Ming HUANG ,  Marty SINACORE ,  Thomas RYLL ,  Jiuyi LU ,  Jaesun LEE ,  Kyngju LEE ,  Hyoung Taek LIM ,  Eunchong YANG

IPC: C12N5/00 ,  C12P21/00

Abstract: The present invention pertains to methods of producing polypeptide of interest in cell cultures lacking glutamine. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with nucleic acid synthesis precursors and/or corticosteroids.