公开(公告)号：US06692971B2

公开(公告)日：2004-02-17

申请号：US09835845

申请日：2001-04-17

申请人： Bori Shushan ,  Mark Kushnir ,  Gabor Komaromy-Hiller

发明人： Bori Shushan ,  Mark Kushnir ,  Gabor Komaromy-Hiller

IPC分类号： G01N2400

CPC分类号： G01N33/82 ,  Y10T436/201666 ,  Y10T436/24

摘要： A novel method for the qualitative and quantitative analysis of dicarboxylic acids in biological samples is provided. The method includes the steps of esterfying the acid component of the sample and subsequently analyzing the esterified sample using tandem mass spectroscopy using atmospheric pressure ionization techniques in the positive ion mode. The method is particularly useful in the determination of methylmalonic acid in biological samples, and thus, the diagnosis of vitamin B12 deficiency.

摘要翻译： 提供了一种用于定性和定量分析生物样品中二羧酸的新方法。 该方法包括以下步骤：酯化样品的酸组分，然后使用串联质谱法利用正离子模式中的大气压电离技术分析酯化样品。 该方法特别适用于生物样品中甲基丙二酸的测定，从而诊断维生素B12缺乏症。

公开(公告)号：US07317187B2

公开(公告)日：2008-01-08

申请号：US11135490

申请日：2005-05-24

申请人： William R. Davidson ,  Bori Shushan ,  Peter Kovarik ,  Tom R. Covey ,  John D. Brennan

发明人： William R. Davidson ,  Bori Shushan ,  Peter Kovarik ,  Tom R. Covey ,  John D. Brennan

IPC分类号： B01D59/44

CPC分类号： H01J49/164 ,  H01J49/0413

摘要： Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.

摘要翻译： 使用含有截留二氢叶酸还原酶的溶胶 - 凝胶衍生的整体硅胶柱用于小分子混合物的正面亲和层析。 来自柱的输出与含有基质分子（HCCA）的第二流组合，并直接沉积到通过计算机控制的x-y阶段相对于柱移动的常规MALDI板上，形成FAC运行的半永久记录。 使用MALDI MS允许FAC和MS方法的去耦，从而允许显着更高的离子强度缓冲液用于FAC研究，这允许在多次运行中更好地保​​留蛋白质活性。

公开(公告)号：US20050269507A1

公开(公告)日：2005-12-08

申请号：US11135490

申请日：2005-05-24

申请人： William Davidson ,  Bori Shushan ,  Peter Kovarik ,  Tom Covey

发明人： William Davidson ,  Bori Shushan ,  Peter Kovarik ,  Tom Covey

IPC分类号： B01D59/44 ,  H01J49/04 ,  H01J49/16

CPC分类号： H01J49/164 ,  H01J49/0413

摘要： Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.

摘要翻译： 使用含有截留二氢叶酸还原酶的溶胶 - 凝胶衍生的整体硅胶柱用于小分子混合物的正面亲和层析。 来自柱的输出与含有基质分子（HCCA）的第二流组合，并直接沉积到通过计算机控制的x-y阶段相对于柱移动的常规MALDI板上，形成FAC运行的半永久记录。 使用MALDI MS允许FAC和MS方法的去耦，从而允许显着更高的离子强度缓冲液用于FAC研究，这允许在多次运行中更好地保​​留蛋白质活性。

公开(公告)号：US20090194685A1

公开(公告)日：2009-08-06

申请号：US12362796

申请日：2009-01-30

申请人： John J. Corr ,  Zoe A. Quinn ,  Bori Shushan

发明人： John J. Corr ,  Zoe A. Quinn ,  Bori Shushan

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/401 ,  G01N30/7233 ,  G01N2030/8411

摘要： Methods and kits for high throughput screening and analysis of metabolic disorders using a laser desorption ion source coupled to a mass analyzer (for example, MALDI) are provided. The metabolic disorders can be amino acid, organic acid or fatty acid oxidation disorders. A panel of disorders can be analyzed at high speeds. The methods and kits are particularly useful for newborn screening (NBS) of metabolic disorders.

摘要翻译： 提供了使用耦合到质量分析器（例如MALDI）的激光解吸离子源进行高通量筛选和代谢紊乱分析的方法和试剂盒。 代谢紊乱可以是氨基酸，有机酸或脂肪酸氧化失调。 可以高速分析一组障碍。 这些方法和试剂盒对代谢紊乱的新生儿筛查（NBS）特别有用。

公开(公告)号：US20070023640A1

公开(公告)日：2007-02-01

申请号：US11133443

申请日：2005-05-20

申请人： William Davidson ,  Bori Shushan

发明人： William Davidson ,  Bori Shushan

IPC分类号： H01J49/00

CPC分类号： G01N30/7286 ,  G01N30/02 ,  B01D15/3804

摘要： Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.

摘要翻译： 使用含有截留二氢叶酸还原酶的溶胶 - 凝胶衍生的整体硅胶柱用于小分子混合物的正面亲和层析。 来自柱的输出与含有基质分子（HCCA）的第二流组合，并直接沉积到通过计算机控制的x-y阶段相对于柱移动的常规MALDI板上，形成FAC运行的半永久记录。 使用MALDI MS允许FAC和MS方法的去耦，从而允许显着更高的离子强度缓冲液用于FAC研究，这允许在多次运行中更好地保​​留蛋白质活性。