公开(公告)号：US09230786B1

公开(公告)日：2016-01-05

申请号：US14301431

申请日：2014-06-11

申请人： Bruker Daltonics, Inc.

发明人： David Kennedy ,  Kerry Nugent

IPC分类号： H01J49/10 ,  H01J49/04 ,  H01J49/16

CPC分类号： H01J49/0404 ,  H01J49/044 ,  H01J49/165 ,  H01J49/167

摘要： The present invention relates to electrospray ionization (ESI) at atmospheric pressure coupled with a mass spectrometer, in particular to a special kind of micro-electrospray with liquid flows in the range of 0.1 to 100 microliters per minute. The invention describes the use of an off-axis pre-entrance channel in an ESI ion source to prevent particulate matter with higher inertia than the (charged) gas molecules, such as droplets, from entering the mass spectrometer. The elimination of the particulate matter improves the quantitative precision of an LC/MS bioassay, minimizes the contamination of the mass spectrometer and improves the robustness for high throughput assays.

摘要翻译： 本发明涉及在大气压力下与质谱联用的电喷雾离子化（ESI），特别涉及一种特殊类型的微电喷雾，液体流速为0.1至100微升/分钟。 本发明描述了在ESI离子源中使用离轴预入口通道来防止比（带电）的气体分子（例如液滴）更高的惯性的颗粒物质进入质谱仪。 颗粒物的消除提高了LC / MS生物测定的定量精度，最大限度地降低了质谱仪的污染，并提高了高通量测定的鲁棒性。