公开(公告)号：US20230099483A1

公开(公告)日：2023-03-30

申请号：US17794704

申请日：2021-01-25

Applicant: C4U CORPORATION ,  OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Kazuto YOSHIMI ,  Satomi SHIBUMURA

IPC: C12Q1/70

Abstract: It has been found that by mixing a single-stranded probe DNA whose cleavage can be detected in a reaction system containing a CRISPR-Cas3 system and a sample from which to detect a target DNA, it is possible to detect the target DNA in the sample by using, as indication, a signal generated by the cleavage of the single-stranded probe DNA.

公开(公告)号：US20240141310A1

公开(公告)日：2024-05-02

申请号：US18279628

申请日：2022-02-25

Applicant: C4U Corporation ,  The University of Tokyo ,  RIKEN

Inventor： Tomoji MASHIMO ,  Kazuto YOSHIMI ,  Kohei TAKESHITA ,  Masaki YAMAMOTO ,  Satomi SHIBUMURA

IPC: C12N9/22 ,  C12N15/85

CPC classification number: C12N9/22 ,  C12N15/85 ,  C12Y301/04 ,  C12N2800/105

Abstract: The present inventors have found that by cultivating insect cells, into which the Cas3 gene has been introduced, at relatively low temperatures, it is possible to efficiently express recombinant Cas3 proteins with maintained activity, and by purifying the soluble fractions of these cells, it is possible to collect active forms of the recombinant Cas3 proteins in high purity and high yield.

公开(公告)号：US20220186263A1

公开(公告)日：2022-06-16

申请号：US17601303

申请日：2020-04-03

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Kazuto YOSHIMI ,  Yoshihiro UNO ,  Yuko KOTANI ,  Yoshiki MIYASAKA ,  Yuichiro OKA ,  Makoto SATO

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10

Abstract: The present inventors have found that the use of a site-specific nuclease system which includes a combination of a molecule that simultaneously targets and cleaves one homology arm sequence of the donor DNA and the genomic sequence corresponding to the homology arm sequence, and a molecule that targets and cleaves the genomic region in the vicinity of the cleavage site by that molecule causes repair between genomic DNA and donor DNA through both non-homologous end joining and homologous recombination, making it possible to produce cells and organisms with knocked-in long donor sequences with high efficiency and accuracy.

公开(公告)号：US20240124898A1

公开(公告)日：2024-04-18

申请号：US18467297

申请日：2023-09-14

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Junji TAKEDA ,  Hiroyuki MORISAKA ,  Kazuto YOSHIMI

IPC: C12N15/90 ,  A61K48/00 ,  C12N5/14 ,  C12N5/16 ,  C12N9/22 ,  C12N15/85

CPC classification number: C12N15/907 ,  A61K48/00 ,  C12N5/14 ,  C12N5/16 ,  C12N9/22 ,  C12N15/85 ,  C12N15/8509 ,  C12N2310/20 ,  C12N2800/22 ,  C12N2800/80

Abstract: A CRISPR-Cas3 system was successfully established in a eukaryotic cell.

公开(公告)号：US20240117381A1

公开(公告)日：2024-04-11

申请号：US18467356

申请日：2023-09-14

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Junji TAKEDA ,  Hiroyuki MORISAKA ,  Kazuto YOSHIMI

IPC: C12N15/90 ,  A61K48/00 ,  C12N5/14 ,  C12N5/16 ,  C12N9/22 ,  C12N15/85

CPC classification number: C12N15/907 ,  A61K48/00 ,  C12N5/14 ,  C12N5/16 ,  C12N9/22 ,  C12N15/85 ,  C12N15/8509 ,  C12N2310/20 ,  C12N2800/22 ,  C12N2800/80

Abstract: A CRISPR-Cas3 system was successfully established in a eukaryotic cell.

公开(公告)号：US20220017888A1

公开(公告)日：2022-01-20

申请号：US17309607

申请日：2019-12-11

Applicant: Kyoto University ,  Osaka University

Inventor： Akitsu HOTTA ,  Yuya OKUZAKI ,  Huaigeng XU ,  Peter David GEE ,  Yuto KITA ,  Tomoji MASHIMO ,  Kazuto YOSHIMI

IPC: C12N15/10 ,  C12N9/22

Abstract: A production method of genomic DNA in which a deletion of more than 100 bases of nucleotides is introduced into a target region of the genomic DNA, the method includes a contact step of bringing a type I CRISPR associated complex for anti-viral defense (type I Cascade complex), CRISPR RNA (crRNA), and Cas3 protein into contact with the genomic DNA.