公开(公告)号：US20030228673A1

公开(公告)日：2003-12-11

申请号：US10375909

申请日：2003-02-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Frances H. Arnold ,  Lian-Hong Sun ,  Ioanna P. Petrounia

IPC: G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  C12N009/02 ,  C12N001/16 ,  C12P021/02 ,  C07H021/04

CPC classification number: C12N9/0006 ,  C12Y101/03004 ,  C12Y101/03009

Abstract: Glucose oxidase enzymes are provided, including novel variants of galactose oxidase enzymes. The polynucleotides that encode these novel variants can be expressed in recombinant host cell expression systems. The novel variant oxidase enzymes are capable of oxidizing compounds towards which wild-type galactose oxidase (e.g. D-galactose: oxygen 6-oxidoreductase, GAO; EC 1.1.3.9) has little or no activity. Preferred galactose oxidase variants are those which that have improved capability to oxidize secondary alcohols and/or D-glucose relative to the wild-type enzyme.

Abstract translation: 提供了葡萄糖氧化酶，包括半乳糖氧化酶的新型变体。 编码这些新变体的多核苷酸可以在重组宿主细胞表达系统中表达。 新型变体氧化酶能够氧化化合物，野生型半乳糖氧化酶（例如D-半乳糖：氧-6-氧化还原酶，GAO; EC1.1.3.9）具有很少或没有活性。 优选的半乳糖氧化酶变体是那些相对于野生型酶具有改善氧化仲醇和/或D-葡萄糖的能力的变体。