公开(公告)号：US20040229367A1

公开(公告)日：2004-11-18

申请号：US10653047

申请日：2003-08-29

申请人： Novozymes Biotech, Inc. ,  Novozymes A/S, Inc.

发明人： Randy M. Berka ,  Michael W. Rey ,  Jeffrey R. Shuster ,  Sakari Kauppinen ,  Ib Groth Clausen ,  Peter Bjarke Olsen

IPC分类号： C12N015/74 ,  C12N001/16

CPC分类号： C12Q1/6895 ,  C07K14/37 ,  C12Q1/6837 ,  C12Q2600/158

摘要： The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing filamentous fungal expressed sequenced tags. The present invention also relates to filamentous fungal expressed sequenced tags and to computer readable media and substrates containing such expressed sequenced tags for monitoring expression of a plurality of genes in filamentous fungal cells.

摘要翻译： 本发明涉及用于使用含有丝状真菌表达的测序标签的微阵列来监测第一丝状真菌细胞中多个基因的差异表达相对于一个或多个第二丝状真菌细胞中相同基因表达的差异表达的方法。 本发明还涉及丝状真菌表达的测序标签以及包含用于监测丝状真菌细胞中多种基因的表达的表达的测序标签的计算机可读介质和底物。

公开(公告)号：US20040229341A1

公开(公告)日：2004-11-18

申请号：US10777460

申请日：2004-02-12

发明人： Marie-France Versali ,  Clerisse Fabienne ,  Carole Gauthier ,  Francoise Dumont ,  Jacques Dommes

IPC分类号： C12N001/16 ,  C12N009/18 ,  C08B037/08 ,  C07H021/04 ,  C12N015/74

CPC分类号： C12N1/16 ,  C07K2319/21 ,  C12N9/80 ,  C12Y305/01041

摘要： In a first aspect, the present invention relates to a recombinant fungal strain, capable of expressing chitin deacetylase, which includes an expression vector that contains a nucleic acid molecule encoding chitin deacetylase, a suitable promoter and a transcription terminator. In particular, the present invention provides a recombinant Aspergillus oryzae strain capable of expressing chitin deacetylase obtained from Mucor rouxii. The present invention also relates to a method for producing chitin deacetylase by a recombinant fungal strain. In a second aspect, the present invention relates to a recombinant yeast strain, capable of expressing chitin deacetylase, which includes an expression vector that contains a nucleic acid molecule encoding chitin deacetylase, a suitable promoter and a transcription terminator. In particular, the present invention provides a recombinant Pichia pastoris strain capable of expressing chitin deacetylase obtained from Mucor rouxii. The present invention also relates to a method for producing chitin deacetylase by a recombinant yeast strain. The present invention further relates to purified recombinant chitin deacetylase enzyme.

摘要翻译： 在第一方面，本发明涉及能够表达几丁质脱乙酰酶的重组真菌菌株，其包括含有编码壳多糖脱乙酰酶的核酸分子，合适的启动子和转录终止子的表达载体。 特别地，本发明提供了能够表达从毛霉（Mucor rouxii）获得的几丁质脱乙酰酶的重组米曲霉菌株。 本发明还涉及通过重组真菌菌株生产壳多糖脱乙酰酶的方法。 在第二方面，本发明涉及能够表达几丁质脱乙酰酶的重组酵母菌株，其包含含有编码甲壳素脱乙酰酶的核酸分子，合适的启动子和转录终止子的表达载体。 特别地，本发明提供能够表达从毛霉（Mucor rouxii）获得的几丁质脱乙酰酶的重组毕赤酵母菌株。 本发明还涉及通过重组酵母菌株生产壳多糖脱乙酰酶的方法。 本发明还涉及纯化的重组壳多糖脱乙酰酶。

公开(公告)号：US20040191864A1

公开(公告)日：2004-09-30

申请号：US10815495

申请日：2004-03-31

申请人： Novozymes Biotech, Inc.

发明人： Mariah Connelly ,  Howard Brody

IPC分类号： C12P021/02 ,  C12P019/34 ,  C12P019/04 ,  C12N009/28 ,  C12N001/16

CPC分类号： C07K14/37 ,  C07K14/38 ,  C07K14/40 ,  C12N9/00 ,  C12N15/52 ,  C12P1/02 ,  C12P21/02

摘要： The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the group consisting of asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase and at least one enzyme selected from the group consisting of acid stable alpha-amylase, neutral alpha-amylase A, and neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Aspergillus niger strains and methods for producing such mutants.

摘要翻译： 本发明涉及产生异源生物物质的方法，包括：（a）在适于生产异源生物物质的培养基中培养母本黑曲霉菌株的突变体，其中（i）突变株包含第一 编码异源生物物质的核苷酸序列和一个或多个第二核苷酸序列，其包含glaA的修饰和至少一种选自asa，amyA，amyB，prtT和oah的基因，以及（ii）突变株 与母本黑曲霉菌株相比，生产葡糖淀粉酶和至少一种选自酸稳定的α-淀粉酶，中性α-淀粉酶A和中性α-淀粉酶B，蛋白酶和草酸水解酶的酶不足 在相同的条件下耕种; 和（b）从培养基中回收异源生物物质。 本发明还涉及黑曲霉菌株的酶缺陷型突变体及其生产方法。

公开(公告)号：US20040105869A1

公开(公告)日：2004-06-03

申请号：US10618337

申请日：2003-07-10

发明人： Kazuo Sakuma

IPC分类号： A61K035/84 ,  C12N001/16 ,  A01G001/04

CPC分类号： A61K36/07

摘要： Preventive and therapeutic agents against the HIV virus and pathogenic bacteria, comprising the active ingredients obtained from hyphae of Kabanoanatake nullscientific name: Fuscoia obliqua (Fr.) Aoshimanull. The said active ingredients have potent inhibitory effects and therapeutic effects on HIV, even in very small amounts. The said active ingredients also have potent inhibitory effects on Bacillus coli, and other pathogenic bacteria. The present invention offers culture methods which enable the supply of active ingredients having potent anti-HIV activity and other physiological activity, by artificial culture of such Kabanoanatake on a large scale.

摘要翻译： 包括从Kabanoanatake（学名：Fuscoia obliqua（Fr.）Aoshima）的菌丝获得的活性成分的HIV病毒和致病细菌的预防和治疗剂。 所述活性成分对艾滋病毒具有有效的抑制作用和治疗效果，即使是非常少的量。 所述活性成分还对大肠杆菌和其他病原菌具有有效的抑制作用。 本发明提供了通过大规模人工培养Kabanoanatake能够提供具有强的抗HIV活性和其它生理活性的活性成分的培养方法。

公开(公告)号：US20040023357A1

公开(公告)日：2004-02-05

申请号：US10439479

申请日：2003-05-16

发明人： Sabine Breinig ,  Wei Wei Qi ,  Fateme Sima Sariaslani ,  Todd M. Vannelli ,  Zhixiong Xue

IPC分类号： C12N009/88 ,  C07H021/04 ,  C12N001/21 ,  C12N001/16 ,  C12N001/18 ,  C12N005/04 ,  C12N015/74

CPC分类号： C12P7/42 ,  C12N9/88

摘要： A novel tyrosine-inducible tyrosine ammonia lyase enzyme was isolated from the yeast Trichosporon cutaneum. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was sequenced using 3null and 5null RACE cloning of the TAL cDNA and the gene was expressed in the yeast Saccharomyces cerevisiae and in the bacterium Escherichia coli.

摘要翻译： 从酵母Trichosporon cutaneum中分离出一种新的酪氨酸诱导型酪氨酸氨裂解酶。 该酶对酪氨酸的活性高于苯丙氨酸，可用于直接从酪氨酸生产对羟基肉桂酸。 使用TAL cDNA的3'和5'RACE克隆对编码该酶的基因进行测序，并且该基因在酵母酿酒酵母和大肠杆菌中表达。

公开(公告)号：US20040014185A1

公开(公告)日：2004-01-22

申请号：US10341220

申请日：2003-01-13

申请人： Danisco Sweeteners Oy

发明人： Heikki Ojamo ,  Merja Penttila ,  Heikki Heikkila ,  Jaana Uusitalo ,  Marja Ilmen ,  Marja-Leena Sarkki ,  Maija-Leena Vehkomaki

IPC分类号： C12P007/18 ,  C12N001/16 ,  C12N001/18

CPC分类号： C12P7/18

摘要： The invention relates to a method for the production of xylitol, the method comprising (a1) providing (i) a microorganism having xylanolytic activity, and (ii) a microorganism capable of converting a pentose sugar to xylitol; or (a2) providing a microorganism having xylanolytic activity and being capable of converting a pentose sugar to xylitol, (b) culturing the microorganism of step (al) (i) or the microorganism of step (a2) in a medium comprising polymer or oligomer materials containing pentose sugars in conditions sufficient for enabling hydrolysis of said polymers or oligomers by the microorganism; (c) producing xylitol in the microorganism of step (a1) (ii) or in the microorganism of step (a2) by bioconversion of the hydrolysis products obtained in step (b), and (d) recovering said xylitol produced. The invention also relates to a microorganism, which has xylanolytic activity and has been genetically modified (i) to enhance its xylanolytic activity, and (ii) to reduce its xylitol metabolism.

摘要翻译： 本发明涉及一种生产木糖醇的方法，所述方法包括（a1）提供（i）具有木聚糖分解活性的微生物和（ii）能够将戊糖转化成木糖醇的微生物; 或（a2）提供具有木聚糖分解活性并能够将戊糖转化为木糖醇的微生物，（b）在包含聚合物或低聚物的培养基中培养步骤（a1）（i）的微生物或步骤（a2）的微生物） 在足以使微生物水解所述聚合物或低聚物的条件下含有戊糖的材料; （c）通过步骤（b）中获得的水解产物的生物转化和（d）回收所生产的木糖醇，在步骤（a1）（ⅱ）的微生物中或在步骤（a2）的微生物中生产木糖醇。 本发明还涉及一种具有木聚糖分解活性且经遗传修饰的微生物（i）增强其木聚糖分解活性，和（ii）降低其木糖醇代谢。

公开(公告)号：US20040009579A1

公开(公告)日：2004-01-15

申请号：US10374247

申请日：2003-02-25

申请人： Novozymes Biotech, Inc.

发明人： Jeffrey R. Shuster ,  John C. Royer

IPC分类号： C12N001/16

CPC分类号： C12N9/2437 ,  C12N9/20 ,  C12N15/01 ,  C12N15/102 ,  C12N15/80 ,  C12R1/69 ,  C12R1/77

摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.

摘要翻译： 本发明涉及从丝状真菌亲本细胞获得突变细胞的方法，包括：（a）获得亲本细胞的突变细胞; （b）鉴定突变细胞，其表现出比母体细胞更加有限的殖民地表型和/或更广泛的菌丝分枝; 和（c）当在相同条件下培养突变体和亲代细胞时，鉴定出与亲本细胞相比具有改进的产生异源多肽性质的突变细胞。

公开(公告)号：US20030207451A1

公开(公告)日：2003-11-06

申请号：US10384918

申请日：2003-03-07

发明人： Eric B. Kmiec ,  Hetal Parekh-Olmedo ,  Erin E. Brachman

IPC分类号： C12N005/08 ,  C12N005/04 ,  C12N001/16 ,  C12N001/18 ,  C12N015/85 ,  C12N015/74

CPC分类号： C12N15/102

摘要： Improved methods, compositions, and kits for oligonucleotide-mediated nucleic acid sequence alteration are presented.

摘要翻译： 提出了用于寡核苷酸介导的核酸序列改变的改进的方法，组合物和试剂盒。

公开(公告)号：US20030186418A1

公开(公告)日：2003-10-02

申请号：US10441626

申请日：2003-05-19

发明人： Peter Gualfetti ,  Colin Mitchinson ,  Jay Phillips

IPC分类号： C12P019/04 ,  C07H021/04 ,  C12N009/42 ,  C12N001/16 ,  C12N015/74

CPC分类号： D06P5/158 ,  C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004 ,  D06M16/003 ,  D06P1/228 ,  D06P5/02 ,  D06P5/137

摘要： The present invention relates to novel variant EGIII or EGIII-like cellulases that have improved stability. The variant cellulases have performance sensitive residues replaced to a residue having modified stability.

摘要翻译： 本发明涉及具有改善的稳定性的新型EGIII或EGIII样纤维素酶。 变性纤维素酶具有性能敏感残基被替换为具有修饰稳定性的残基。

公开(公告)号：US20030180953A1

公开(公告)日：2003-09-25

申请号：US10032585

申请日：2001-12-20

申请人： Elitra Pharmaceuticals, Inc.

发明人： Terry Roemer ,  Bo Jiang ,  Charles Boone ,  Howard Bussey ,  Kari L. Ohlsen

IPC分类号： C12N001/18 ,  C12N001/16 ,  C12N015/74

CPC分类号： C12N15/1082 ,  C07K14/37 ,  C07K14/40 ,  C12R1/645 ,  C12R1/725

摘要： The present invention provides methods and compositions that enable the experimental determination as to whether any gene in the genome of a diploid pathogenic organism is essential, and whether it is required for virulence or pathogenicity. The methods involve the construction of genetic mutants in which one allele of a specific gene is inactivated while the other allele of the gene is placed under conditional expression. The identification of essential genes and those genes critical to the development of virulent infections, provides a basis for the development of screens for new drugs against such pathogenic organisms. The present invention further provides Candida albicans genes that are demonstrated to be essential and are potential targets for drug screening. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic acid arrays. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention.

摘要翻译： 本发明提供了能够对二倍体致病生物体的基因组中的任何基因是否必需的实验测定以及是否需要毒力或致病性的方法和组合物。 该方法包括构建遗传突变体，其中特定基因的一个等位基因被灭活而基因的其他等位基因置于条件表达下。 识别必需基因和对致病性感染发展至关重要的那些基因，为开发针对这种致病生物的新药筛选提供了基础。 本发明进一步提供了被证实是必需的白念珠菌基因，并且是药物筛选的潜在靶标。 靶基因的核苷酸序列可以用于各种药物发现目的，例如重组蛋白的表达，杂交测定和核酸阵列的构建。 在各种筛选方法中，由必需基因编码的蛋白质的使用以及包含必需基因的修饰的等位基因的遗传工程改造的细胞也包括在本发明中。