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    Mutants of enzymes and methods for their use
    8.
    发明申请
    Mutants of enzymes and methods for their use 审中-公开
    酶的突变体及其使用方法

    公开(公告)号：US20040115691A1

    公开(公告)日：2004-06-17

    申请号：US10617998

    申请日：2003-07-10

    发明人： J. David Rozzell ,  Ling Hua ,  Martin Mayhew ,  Scott Novick

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/06

    CPC分类号： C12Y104/01009 ,  C07H21/04 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0016 ,  C12Y101/03009 ,  C12Y102/01002

    摘要： Mutants of leucine dehydrogenase sequences, formate dehydrogenase sequences and galactose oxidase sequences are provided. An amino acid sequence that is a mutant of a leucine dehydrogenase sequence as described in SEQ ID 2, or its substantial equivalent, contains at least one mutation selected from the group consisting of F102S, V33A, S351T, N145S and like mutations in subsantially equivalent sequences. An amino acid sequence that is a mutant of a formate dehydrogenase sequence as described in SEQ ID 1, or its substantial equivalent, contains at least one mutation selected from the group consisting of D195S, Y196H, K356T and like mutations in subsantially equivalent sequences. An amino acid sequence that is a mutant of a galactose oxidase sequence as described in SEQ ID 3, or its substantial equivalent, contains at least one mutation selected from the group consisting of N25Y, T94A, D216N, R217C, M278T, Y329C, Q406R, Q406L, V492A, V494A, N521S, N535D, T5491, S567T, T578S and like mutations in subsantially equivalent sequences. Deoxyribonucleic acid molecules containing DNA sequences encoding these mutants are also provided.

    摘要翻译： 提供亮氨酸脱氢酶序列，甲酸脱氢酶序列和半乳糖氧化酶序列的突变体。 作为SEQ ID No.2所示的亮氨酸脱氢酶序列的突变体的氨基酸序列或其实质等价物，含有选自F102S，V33A，S351T，N145S等的至少一个突变，以及亚等同序列中的类似突变 。 作为SEQ ID No.1所示的甲酸脱氢酶序列的突变体的氨基酸序列或其实质等价物，含有选自D195S，Y196H，K356T等的至少一个突变，以及亚等同序列中的类似突变。 作为SEQ ID 3所示的半乳糖氧化酶序列的突变体的氨基酸序列或其实质等价物，含有选自N25Y，T94A，D216N，R217C，M278T，Y329C，Q406R， Q406L，V492A，V494A，N521S，N535D，T5491，S567T，T578S等突变等位基因序列。 还提供了含有编码这些突变体的DNA序列的脱氧核糖核酸分子。

    Enzymatic powder milk
    9.
    发明授权
    Enzymatic powder milk 失效
    酶粉奶

    公开(公告)号：US4617190A

    公开(公告)日：1986-10-14

    申请号：US644395

    申请日：1984-08-27

    申请人： Robert E. Montgomery

    发明人： Robert E. Montgomery

    IPC分类号： A23C9/12 ,  A23C9/16

    CPC分类号： C12Y101/03004 ,  A23C9/1213 ,  C12Y101/03005 ,  C12Y101/03009 ,  C12Y101/0301 ,  C12Y102/03003 ,  C12Y104/03002 ,  C12Y104/03003 ,  C12Y111/01 ,  C12Y111/01008 ,  C12Y111/0101 ,  Y10S426/801

    摘要： Aqueous reconstitutible, powder milk incorporates an enzyme system for providing a bacteriostatic effect upon aqueous reconstitution. The enzyme system contains (a) oxidoreductase enzyme that is hydro-interactable with and specific to oxidizable substrate in the powder milk for producing hydrogen peroxide and (b) peroxidatic peroxidase for interacting with the hydrogen peroxide and oxidizable anion from the powder milk to produce, in the reconstituted milk, oxidized anionic bacterial inhibitor. In an illustrative embodiment, powder milk incorporates (a) glucose oxidase that interacts with glucose in powder milk, upon aqueous dilution, to produce hydrogen peroxide and (b) lactoperoxidase for interacting with hydrogen peroxide and, for example the chloride ion from the powder milk to produce, in the reconstituted milk, the hypochlorite ion, a bacterial inhibitor.

    摘要翻译： 水性可重构的粉末奶结合了一种酶系统，用于在水重构时提供抑菌作用。 酶系统包含（a）氧化还原酶，其与用于产生过氧化氢的粉末乳中可氧化底物水合相互作用和特异性氧化还原酶，和（b）过氧化物过氧化物酶与来自粉末奶的过氧化氢和可氧化阴离子相互作用， 在复原乳中，氧化阴离子细菌抑制剂。 在一个示例性实施方案中，粉末奶合并（a）在水性稀释时与粉末状乳中的葡萄糖相互作用的葡萄糖氧化酶，以产生过氧化氢，和（b）与过氧化氢相互作用的乳过氧化物酶，例如来自粉末奶的氯离子 在再生牛奶中产生次氯酸根离子，即细菌抑制剂。