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    Apparatus and method for increasing insect resistance in transgenic plants
    1.
    发明申请
    Apparatus and method for increasing insect resistance in transgenic plants 审中-公开
    用于增加转基因植物抗虫性的装置和方法

    公开(公告)号：US20040259080A1

    公开(公告)日：2004-12-23

    申请号：US09956326

    申请日：2001-09-18

    发明人： Gary W. Felton ,  Yinong Yang ,  Qin Wang

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/02

    CPC分类号： A01N37/46 ,  A01N63/02 ,  C12N9/0006 ,  C12Q1/26

    摘要： The present invention discloses a polynucleotide sequence for an insect salivary glucose oxidase enzyme and the amino acid sequence of the enzyme itself. It also provides recombinant polynucleotide vector systems designed to express the enzyme in a variety of host organisms. The invention also discloses a method for creating transgenic plants having increased resistance to insect predation resulting from the expression of the foreign glucose oxidase protein. The presence of the insect glucose oxidase enzyme triggers a plant's defensive mechanisms and results in increased resistance to insects.

    摘要翻译： 本发明公开了一种昆虫唾液葡萄糖氧化酶的多核苷酸序列和酶本身的氨基酸序列。 它还提供设计用于在多种宿主生物体中表达酶的重组多核苷酸载体系统。 本发明还公开了一种产生转基因植物的方法，所述转基因植物具有增加的抗外源葡萄糖氧化酶蛋白质表达引起的昆虫捕食的能力。 昆虫葡萄糖氧化酶的存在触发植物的防御机制，并导致对昆虫的抗性增加。

    Mitogenic oxygenase regulators
    2.
    发明申请
    Mitogenic oxygenase regulators 有权
    有丝分裂加氧酶调节剂

    公开(公告)号：US20040253681A1

    公开(公告)日：2004-12-16

    申请号：US10850060

    申请日：2004-05-20

    发明人： J. David Lambeth ,  Guangjie Cheng

    IPC分类号： C12N009/02 ,  C07H021/04

    CPC分类号： C12N9/0036 ,  A01K2217/05 ,  C07K16/40 ,  C12N9/0004 ,  C12N2799/026 ,  G01N33/573 ,  G01N2333/90209

    摘要： The present invention relates to new genes encoding for the production of novel nox enzyme proteins involved in generation of reactive oxygen intermediates that affect cell division. The present invention also provides vectors containing these genes, cells transfected with these vectors, antibodies raised against these novel proteins, kits for detection, localization and measurement of these genes and proteins, and methods to determine the activity of drugs to affect the activity of the proteins of the present invention.

    摘要翻译： 本发明涉及编码产生影响细胞分裂的活性氧中间体的新型nox酶蛋白的新基因。 本发明还提供含有这些基因的载体，用这些载体转染的细胞，针对这些新蛋白产生的抗体，用于检测，定位和测量这些基因和蛋白质的试剂盒，以及确定药物活性以影响其活性的方法 本发明的蛋白质。

    Composition for detecting peptidoglycan, and diagnostic kit detecting peptidoglycan
    3.
    发明申请
    Composition for detecting peptidoglycan, and diagnostic kit detecting peptidoglycan 审中-公开
    用于检测肽聚糖的组合物和诊断试剂盒检测肽聚糖

    公开(公告)号：US20040248271A1

    公开(公告)日：2004-12-09

    申请号：US10479910

    申请日：2003-12-05

    发明人： Bu-Soo Park ,  Chang-Hun Joo ,  Moon-Suk Kim ,  Seung-Hwan Song ,  Jong-Won Yoon ,  Yeon-Sung Park ,  Hong-Lak Kim ,  Joong-Hyuck Auh ,  Tae-Hoon Cho ,  Bok-Luel Lee ,  Ji-Won Park ,  Jeong-Mi Yeo ,  Hyun-Sic Kim

    IPC分类号： C12N009/02 ,  A61K035/64

    CPC分类号： G01N33/56911 ,  C12Q1/04 ,  C12Q1/26 ,  G01N2333/43552

    摘要： The present invention relates to a composition for selectively detecting an extremely small amount of peptidoglycan in sample, a preparation method of the composition, and a detection kit for peptidoglycan. It is possible to quantify a small amount of peptidoglycan contained in human blood, tissue, body fluid, water or food, and to diagnose an infection of microorganism with peptidoglycan as a component of cell wall using the composition and the detection kit. In addition, the composition can be applied for a diagnosis reagent of detecting an infection of Gram-positive bacteria in animal or human being in advance, and thus, can be used for the prevention or treatment of food poisonings and Bacterial sepsis.

    摘要翻译： 本发明涉及用于选择性地检测样品中极少量的肽聚糖的组合物，该组合物的制备方法和用于肽聚糖的检测试剂盒。 使用组合物和检测试剂盒，可以量化少量的人血液，组织，体液，水或食物中的肽聚糖，并利用肽聚糖作为细胞壁的组分来诊断微生物的感染。 此外，该组合物可以预先用于检测动物或人类革兰氏阳性菌感染的诊断试剂，因此可用于预防或治疗食物中毒和细菌性败血症。

    Mutant P53 proteins and uses thereof
    6.
    发明申请
    Mutant P53 proteins and uses thereof 审中-公开
    突变型P53蛋白及其用途

    公开(公告)号：US20040175813A1

    公开(公告)日：2004-09-09

    申请号：US10696255

    申请日：2003-10-29

    发明人： Kimberly Kline ,  Bob G. Sanders ,  Weiping Yu

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/02

    CPC分类号： C07K14/4746 ,  A61K38/00 ,  A61K48/00

    摘要： Mutated p53 proteins comprising amino acids deletion were disclosed herein. The mutant p53 exhibits high cellular retention and is capable of rendering tumor cells sensitive to apoptotic inducing agents such as null-irradiation or chemotherapeutic agents. The mutant p53 protein can be delivered separately or in combination with apoptotic inducing agents via aerosol liposome/transfection/infection methods to treat cellular proliferative diseases and disorders in humans and animals.

    摘要翻译： 本文公开了包含氨基酸缺失的突变的p53蛋白。 突变体p53表现出高细胞保留性，并且能够使肿瘤细胞对凋亡诱导剂如γ辐射或化学治疗剂敏感。 突变型p53蛋白可以通过气溶胶脂质体/转染/感染方法单独递送或与凋亡诱导剂组合递送以治疗人和动物中的细胞增殖性疾病和病症。

    Alcohol dehydrogenases with increased solvent and temperature stability
    7.
    发明申请
    Alcohol dehydrogenases with increased solvent and temperature stability 有权
    醇脱氢酶具有增加的溶剂和温度稳定性

    公开(公告)号：US20040157305A1

    公开(公告)日：2004-08-12

    申请号：US10666205

    申请日：2003-09-18

    发明人： Wolfgang Stampfer ,  Birgit Kosjek ,  Wolfgang Kroutil ,  Kurt Faber ,  Jurgen Eck ,  Frank Niehaus

    IPC分类号： C12N001/21 ,  C12N009/02

    CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P41/002

    摘要： The invention relates to biocatalysts showing alcohol dehydrogenase activity obtainable from Rhodococcus ruber, their preparation, their use in the oxidation of secondary alcohols and/or the reduction of ketones, as well as nucleic acids coding for these alcohol dehydrogenases and microorganisms transformed with nucleic acids coding for these biocatalysts and their use for producing the biocatalyst or oxidizing secondary alcohols and/or reducing ketones.

    摘要翻译： 本发明涉及显示可从红球菌获得的醇脱氢酶活性的生物催化剂，它们的制备，它们在仲醇氧化中的用途和/或还原酮，以及编码这些醇脱氢酶的核酸和用编码的核酸转化的微生物 这些生物催化剂及其用于生产生物催化剂或氧化仲醇和/或还原酮的用途。

    Methods of screening for cox-2 inhibitors
    8.
    发明申请
    Methods of screening for cox-2 inhibitors 审中-公开
    筛选cox-2抑制剂的方法

    公开(公告)号：US20040152126A1

    公开(公告)日：2004-08-05

    申请号：US10806634

    申请日：2004-03-23

    发明人： Manuel Fresno Escudero ,  Miguel Angel Iniguez Pena

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/02 ,  C12N009/10 ,  C12N005/08

    CPC分类号： C12N9/0083 ,  C12Q1/6897

    摘要： The cell lines comprises a DNA construct that comprises all or part of a promoter sequence of the gene coding for cyclooxygenase 2 (cox-2) and a reporter gene, operatively joined to each other, such that said promoter sequence of the cox-2 gene controls the expression of said reporter gene in response to a suitable stimulus. The assay method comprises bringing said cell line into contact with the compound to be assayed and determining the existence of a signal indicative of the expression of activity due to the reporter gene. This method is suitable for the induction at a transcriptional level of cox-2 by suitable stimuli.

    摘要翻译： 细胞系包含DNA构建体，其包含编码环氧合酶2（cox-2）的基因的启动子序列和可操作地连接的报告基因的全部或部分，使得所述cox-2基因的启动子序列 响应于合适的刺激控制所述报道基因的表达。 测定方法包括使所述细胞系与待测化合物接触，并确定存在指示由于报告基因导致的活性表达的信号。 该方法适用于通过合适的刺激诱导cox-2的转录水平。