公开(公告)号：US20150327537A1

公开(公告)日：2015-11-19

申请号：US14431621

申请日：2012-09-27

Applicant: CEFO CO., LTD.

Inventor： Sunray Lee ,  Jin Yup Lee ,  Hyun Jung Mo ,  Hyun-Sook Park

IPC: A01N1/02

CPC classification number: A01N1/0221 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/0606

Abstract: The present invention relates to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, and use thereof, more particularly, to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, in which the albumin, lipids, and plant protein hydrolysates are present in sufficient quantities to reduce cell death caused by freezing while maintaining animal-free and xeno-free conditions during long-term preservation of stem cells or primary cells; and to a method for cryopreservation of stem cells or primary cells using the composition.

Abstract translation: 本发明涉及一种包含植物来源的重组人血清白蛋白，脂质和植物蛋白水解物作为干细胞或原代细胞的低温保存活性成分的组合物，更具体地涉及包含植物来源的重组人 血清白蛋白，脂质和植物蛋白水解物作为干细胞或原代细胞的低温保存的活性成分，其中白蛋白，脂质和植物蛋白水解物以足够的量存在，以减少冻结引起的细胞死亡，同时保持无动物和 在干细胞或原代细胞的长期保存期间不含异种病毒的条件; 以及使用该组合物冷冻保存干细胞或原代细胞的方法。

公开(公告)号：US11634675B2

公开(公告)日：2023-04-25

申请号：US16770663

申请日：2018-12-07

Applicant: Korea Institute of Industrial Technology ,  CEFO Co., Ltd.

Inventor： Dongmok Lee ,  Hyunsook Park ,  Sunray Lee

IPC: C12M1/00 ,  C12M1/32 ,  C12N5/00

Abstract: The present invention relates to an insertable culture container and a kit for three-dimensional cell culture, and a three-dimensional cell co-culture method using the same, the insertable culture container for three-dimensional cell culture comprising: a cylindrical side wall having open upper and lower portions; at least one hook protruding outward from the upper side of the side wall; and at least one support protruding inward from the lower side of the side wall. The present invention is advantageous in that air required for a three-dimensional cell culture structure can be smoothly supplied since the cell is cultured at a position spaced apart from a bottom surface of the culture container, and an existing culture plate can be used without change due to the culture container configured as an insert type.

公开(公告)号：US10104881B2

公开(公告)日：2018-10-23

申请号：US14431621

申请日：2012-09-27

Applicant: CEFO Co., Ltd.

Inventor： Sunray Lee ,  Jin Yup Lee ,  Hyun Jung Mo ,  Hyun-Sook Park

IPC: A01N1/02 ,  C12N5/07 ,  C12N5/0735

Abstract: The present invention relates to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, and use thereof, more particularly, to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, in which the albumin, lipids, and plant protein hydrolysates are present in sufficient quantities to reduce cell death caused by freezing while maintaining animal-free and xeno-free conditions during long-term preservation of stem cells or primary cells; and to a method for cryopreservation of stem cells or primary cells using the composition.

公开(公告)号：US11802263B2

公开(公告)日：2023-10-31

申请号：US16640849

申请日：2018-08-22

Applicant: KOREA INSTITUTE OF INDUSTRIAL TECHNOLOGY ,  CEFO CO., LTD. ,  KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY ,  KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Dongmok Lee ,  Woojong Lee ,  Woojin Kim ,  Hyunsook Park ,  Sunray Lee ,  Kiyoung Kim ,  Sungbum Park ,  Kwangrok Kim ,  Byumseok Koh ,  Kyongjin Choi ,  Hyejin Nam ,  Dukjin Kang

IPC: C12M1/32 ,  C12N5/0775

CPC classification number: C12M1/32 ,  C12M23/12 ,  C12N5/0667 ,  C12N2502/1157 ,  C12N2502/1382 ,  C12N2513/00 ,  C12N2533/40

Abstract: The present invention relates to a culture vessel for three-dimensional cell cultivation and a three-dimensional cell co-cultivation method using the same. The culture vessel comprises a well formed by a column positioned thereon and at least one support protruding from the column within the well. In contrast to conventional techniques, the present invention allows cells to be cultured at a position spaced from a culture vessel, thus enjoying the advantage of smoothly supplying oxygen necessary for three-dimensional cell culture structures.