公开(公告)号：US20190316100A1

公开(公告)日：2019-10-17

申请号：US16237901

申请日：2019-01-02

Applicant: CELLECTIS

Inventor： Philippe Duchateau ,  Alexandre Juillerat ,  Julien Valton ,  Claudia Bertonati ,  Jean-Charles Epinat ,  George H. Silva

IPC: C12N9/16 ,  C12P19/34 ,  C07K14/47 ,  C12N9/22

Abstract: The present invention relates to new Transcription Activator-Like Effector proteins and more particularly new Transcription Activator-Like Effector Nucleases (TALENs) that can efficiently target and process nucleic acids. The present invention also concerns methods to use these new Transcription Activator-Like Effector proteins. The present invention also relates to vectors, compositions and kits in which Transcription Activator-Like Effector proteins of the present invention are used.

公开(公告)号：US11466291B2

公开(公告)日：2022-10-11

申请号：US16314697

申请日：2017-06-30

Applicant: CELLECTIS

Inventor： Jean-Pierre Cabaniols ,  Jean-Charles Epinat ,  Philippe Duchateau

IPC: C12N15/86 ,  C12N5/0783 ,  C12N9/22 ,  C12N13/00 ,  C12N15/113 ,  C12N15/90

Abstract: The invention pertains to the field of adaptive cell immunotherapy. It aims at reducing the occurrence of translocations and cell deaths when several specific endonuclease reagents are used altogether to genetically modify primary immune cells at different genetic loci. The method of the invention allows to yield safer immune primary cells harboring several genetic modifications, such as triple or quadruple gene inactivated cells, from populations or sub-populations of cells originating from a single donor or patient, for their subsequent use in therapeutic treatments.

公开(公告)号：US10087453B2

公开(公告)日：2018-10-02

申请号：US14899107

申请日：2014-06-25

Applicant: CELLECTIS

Inventor： Philippe Duchateau ,  Fayza Daboussi ,  David Sourdive ,  Jean-Charles Epinat

IPC: C12N15/82 ,  C12P7/64 ,  C12N9/16 ,  C12N9/12 ,  C12N9/10 ,  C12N9/02 ,  C12N9/04 ,  C12N15/79

Abstract: The invention provides engineered diatoms and methods of producing oil using diatoms. The invention also provides methods of modifying the lipids quantity and/or quality produced by diatom organisms through genome engineering. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured from such modified diatoms.

公开(公告)号：US11136566B2

公开(公告)日：2021-10-05

申请号：US16237901

申请日：2019-01-02

Applicant: CELLECTIS

Inventor： Philippe Duchateau ,  Alexandre Juillerat ,  Julien Valton ,  Claudia Bertonati ,  Jean-Charles Epinat ,  George H. Silva

IPC: C12N9/16 ,  C12N15/63 ,  C12N5/071 ,  C12N5/10 ,  C07K14/195 ,  C12N9/22 ,  C07K14/47 ,  A61K38/00 ,  C12P19/34

Abstract: The present invention relates to new Transcription Activator-Like Effector proteins and more particularly new Transcription Activator-Like Effector Nucleases (TALENs) that can efficiently target and process nucleic acids. The present invention also concerns methods to use these new Transcription Activator-Like Effector proteins. The present invention also relates to vectors, compositions and kits in which Transcription Activator-Like Effector proteins of the present invention are used.

公开(公告)号：US11674155B2

公开(公告)日：2023-06-13

申请号：US17817877

申请日：2022-08-05

Applicant: CELLECTIS

Inventor： Jean-Pierre Cabaniols ,  Jean-Charles Epinat ,  Philippe Duchateau

IPC: C12N15/86 ,  C12N5/0783 ,  C12N9/22 ,  C12N13/00 ,  C12N15/113 ,  C12N15/90

CPC classification number: C12N15/86 ,  C12N5/0636 ,  C12N9/22 ,  C12N13/00 ,  C12N15/113 ,  C12N15/902 ,  C12N2310/20 ,  C12N2510/00 ,  C12N2800/80

Abstract: The invention pertains to the field of adaptive cell immunotherapy. It aims at reducing the occurrence of translocations and cell deaths when several specific endonuclease reagents are used altogether to genetically modify primary immune cells at different genetic loci. The method of the invention allows to yield safer immune primary cells harboring several genetic modifications, such as triple or quadruple gene inactivated cells, from populations or sub-populations of cells originating from a single donor or patient, for their subsequent use in therapeutic treatments.

公开(公告)号：US20220010292A1

公开(公告)日：2022-01-13

申请号：US17489454

申请日：2021-09-29

Applicant: CELLECTIS

Inventor： Philippe Duchateau ,  Julien Valton ,  Claudia Bertonati ,  Jean-Charles Epinat ,  George H. Silva ,  Alexandre Juillerat ,  Marine Beurdeley

IPC: C12N9/16 ,  C12N15/63 ,  C07K14/195 ,  C12N5/071 ,  C12N5/10 ,  C12N9/22

Abstract: The present invention relates to a method for the generation of compact Transcription Activator-Like Effector Nucleases (TALENs) that can efficiently target and process double-stranded DNA. More specifically, the present invention concerns a method for the creation of TALENs that consist of a single TALE DNA binding domain fused to at least one catalytic domain such that the active entity is composed of a single polypeptide chain for simple and efficient vectorization and does not require dimerization to target a specific single double-stranded DNA target sequence of interest and process DNA nearby the DNA target sequence. The present invention also relates to compact TALENs, vectors, compositions and kits used to implement the method.

公开(公告)号：US11198856B2

公开(公告)日：2021-12-14

申请号：US15069672

申请日：2016-03-14

Applicant: CELLECTIS

Inventor： Philippe Duchateau ,  Julien Valton ,  Claudia Bertonati ,  Jean-Charles Epinat ,  George H. Silva ,  Alexandre Juillerat ,  Marine Beurdeley

IPC: C12N9/16 ,  C12N15/63 ,  C07K14/195 ,  C12N9/22 ,  C07K14/47 ,  A61K38/00 ,  C12P19/34

Abstract: The present invention relates to a method for the generation of compact Transcription Activator-Like Effector Nucleases (TALENS) that can efficiently target and process double-stranded DNA. More specifically, the present invention concerns a method for the creation of TALENs that consist of a single TALE DNA binding domain fused to at least one catalytic domain such that the active entity is composed of a single polypeptide chain for simple and efficient vectorization and does not require dimerization to target a specific single double-stranded DNA target sequence of interest and process DNA nearby the DNA target sequence. The present invention also relates to compact TALENs, vectors, compositions and kits used to implement the method.

公开(公告)号：US20160298098A1

公开(公告)日：2016-10-13

申请号：US15069672

申请日：2016-03-14

Applicant: CELLECTIS, S.A.

Inventor： Philippe Duchateau ,  Julien Valton ,  Claudia Bertonati ,  Jean-Charles Epinat ,  George H. Silva ,  Alexandre Juillerat ,  Marine Beurdeley

IPC: C12N9/22 ,  C07K14/47 ,  C12P19/34

CPC classification number: C12N9/16 ,  A61K38/00 ,  C07K14/4703 ,  C07K2319/00 ,  C07K2319/80 ,  C12N9/22 ,  C12P19/34

Abstract: The present invention relates to a method for the generation of compact Transcription Activator-Like Effector Nucleases (TALENS) that can efficiently target and process double-stranded DNA. More specifically, the present invention concerns a method for the creation of TALENs that consist of a single TALE DNA binding domain fused to at least one catalytic domain such that the active entity is composed of a singe polypeptide chain for simple and efficient vectorization and does not require dimerization to target a specific single double-stranded DNA tar et sequence of interest and process DNA nearby said DNA target sequence. The present invention also relates to compact TALENs, vectors, compositions and kits used to implement the method.

Abstract translation: 本发明涉及能够有效靶向和加工双链DNA的致密转录激活子样效应核酸酶（TALENS）的生成方法。 更具体地，本发明涉及一种用于产生TALEN的方法，其由与至少一个催化结构域融合的单个TALE DNA结合结构域组成，使得活性实体由简单且有效的载体化的单个多肽链组成，并且不 需要二聚化来靶向目标特定的单链双链DNA焦油和序列，并在DNA附近处理DNA靶序列。 本发明还涉及用于实施该方法的紧凑型TALEN，载体，组合物和试剂盒。