公开(公告)号：US20150291967A1

公开(公告)日：2015-10-15

申请号：US14439413

申请日：2013-10-31

Applicant: Luc MATHIS ,  Daniel VOYTAS ,  Jin LI ,  Feng ZHANG ,  Song LUO ,  CELLECTIS

Inventor： Luc Mathis ,  Daniel F. Voytas ,  Jin Li ,  Feng Zhang ,  Song Luo

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8209 ,  C12N15/8274 ,  C12N15/8278

Abstract: The present invention relates to methods allowing the targeted insertion of transgenes into a plant genome at desired loci by using homologous recombination combined with rare-cutting endonucleases without the need of inserting an exogenous selectable marker.

Abstract translation: 本发明涉及通过使用与稀有切割内切核酸酶组合的同源重组而不需要插入外源选择性标记的方法，允许将转基因靶向插入到所需基因座的植物基因组中。

公开(公告)号：US11312972B2

公开(公告)日：2022-04-26

申请号：US16461553

申请日：2017-11-16

Applicant: CELLECTIS

Inventor： Nicholas Baltes ,  Song Luo

IPC: C12N15/82 ,  C12N15/52

Abstract: Materials and methods are provided for making plants with altered levels of amino acids, particularly by making controlled frameshift mutations in genes that are highly expressed in plant leaves or plant seeds.

公开(公告)号：US11479782B2

公开(公告)日：2022-10-25

申请号：US16608060

申请日：2018-04-25

Applicant: CELLECTIS

Inventor： Song Luo ,  Nicholas J. Baltes

IPC: C12N15/82

Abstract: Materials and methods for creating plants (e.g., alfalfa lines) with reduced lignin content and composition are provided herein, as are plants, plant parts, and plant cells generated by the methods provided herein.

公开(公告)号：US20210002656A1

公开(公告)日：2021-01-07

申请号：US17022421

申请日：2020-09-16

Applicant: CELLECTIS

Inventor： Daniel F. Voytas ,  Feng Zhang ,  Jin Li ,  Thomas Stoddard ,  Song Luo

IPC: C12N15/82 ,  C12N9/22

Abstract: Materials and methods for creating genome-engineered plants with non-transgenic methods are provided herein.

公开(公告)号：US20200048648A1

公开(公告)日：2020-02-13

申请号：US16608060

申请日：2018-04-25

Applicant: CELLECTIS

Inventor： Song Luo ,  Nicholas J. Baltes

IPC: C12N15/82

Abstract: Materials and methods for creating plants (e.g., alfalfa lines) with reduced lignin content and composition are provided herein, as are plants, plant parts, and plant cells generated by the methods provided herein.

公开(公告)号：US20200002709A1

公开(公告)日：2020-01-02

申请号：US16328323

申请日：2017-08-30

Applicant: CELLECTIS

Inventor： Song Luo ,  Benjamin Clasen ,  Feng Zhang ,  Nicholas J. Baltes ,  Javier Gil Humanes

IPC: C12N15/82

Abstract: Materials and methods are provided for making plants (e.g., soybean varieties, wheat varieties, or corn varieties) with altered amino acid content. For example, materials and methods are provided for making TALE nuclease-induced mutations in genes encoding seed storage proteins, or by making TALE nuclease-induced deletions of within seed storage protein genes.

公开(公告)号：US20190211349A1

公开(公告)日：2019-07-11

申请号：US16326549

申请日：2017-08-18

Applicant: CELLECTIS

Inventor： Benjamin Clasen ,  Song Luo ,  Daniel F. Voytas ,  Feng Zhang

IPC: C12N15/82 ,  C12N15/52

CPC classification number: C12N15/8261 ,  C12N9/0059 ,  C12N15/52 ,  C12N15/8242 ,  C12Y110/03001

Abstract: Materials and methods are provided for making plants (e.g., Solanum varieties) with resistance to black-spot bruising, specifically, by making TALE-nuclease-induced mutations in genes encoding tuber specific expressed polyphenol oxidase.

公开(公告)号：US20170081672A1

公开(公告)日：2017-03-23

申请号：US15056544

申请日：2016-02-29

Applicant: CELLECTIS

Inventor： Thomas Stoddard ,  Song Luo

IPC: C12N15/52 ,  A01H4/00 ,  C12N9/22

CPC classification number: C12N15/52 ,  A01H4/008 ,  C12N9/22 ,  C12N15/8241 ,  C12N2840/10 ,  C12Y301/00

Abstract: Materials and methods for genome engineering through transient expression of a targeted nuclease are described herein. For example, the methods described herein can include introducing into a cell a messenger RNA (mRNA) that encodes a nuclease targeted to a selected sequence within the cell, where the stability of the mRNA is modified by the addition of untranslated regions (UTRs).

公开(公告)号：US20190264220A1

公开(公告)日：2019-08-29

申请号：US16461553

申请日：2017-11-16

Applicant: CELLECTIS

Inventor： Nicholas Baltes ,  Song Luo

IPC: C12N15/82 ,  C12N15/52

Abstract: Materials and methods are provided for making plants with altered levels of amino acids, particularly by making controlled frameshift mutations in genes that are highly expressed in plant leaves or plant seeds.

公开(公告)号：US20160222395A1

公开(公告)日：2016-08-04

申请号：US15013323

申请日：2016-02-02

Applicant: CELLECTIS

Inventor： Thomas Stoddard ,  Nicholas J. Baltes ,  Song Luo

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8213 ,  C12N15/8245 ,  C12N15/8251 ,  C12N15/8282

Abstract: Methods for genome engineering, including methods utilizing transient expression of a nuclease utilizing modified transfer-DNA (T-DNA) plasmids, are provided herein.

Abstract translation: 本文提供了基因组工程方法，包括利用修饰的转移DNA（T-DNA）质粒瞬时表达核酸酶的方法。