公开(公告)号：US20140212470A1

公开(公告)日：2014-07-31

申请号：US14228212

申请日：2014-03-27

申请人： CELLSEED INC.

发明人： Masato KANZAKI ,  Masayuki YAMATO ,  Tinatsu KOHNO ,  Hidekazu SEKINE ,  Akihiko KIKUCHI ,  Teruo OKANO ,  Takamasa ONUKI

IPC分类号： A61L27/38 ,  C12N5/071

CPC分类号： A61L27/3895 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  C12N5/0068 ,  C12N5/0688 ,  C12N2533/30

摘要： The problem to be solved by the present invention is to provide a cultured cell sheet having a superior tissue adhesiveness and an excellent flexibility. The problems can be solved by producing the cultured cell sheet in which cells are cultured in the presence of a surfactant protein or a cross-linking inhibitor on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; and detaching the cultured cell sheet.

摘要翻译： 本发明要解决的问题是提供具有优异组织粘附性和优异柔韧性的培养细胞片。 可以通过在表面活性蛋白或交联抑制剂的存在下培养细胞培养的细胞片来解决问题，所述细胞片在涂覆有具有上或下的温度响应性聚合物的细胞培养载体的表面上 包括以下步骤：将培养基的温度调节到低于临界溶解温度以下或高于临界溶解温度以上的温度; 并分离培养的细胞片。