公开(公告)号：US09889228B2

公开(公告)日：2018-02-13

申请号：US14228212

申请日：2014-03-27

Applicant: CELLSEED INC.

Inventor： Masato Kanzaki ,  Masayuki Yamato ,  Tinatsu Kohno ,  Hidekazu Sekine ,  Akihiko Kikuchi ,  Teruo Okano ,  Takamasa Onuki

IPC: A61L27/38 ,  A61L27/34 ,  C12N5/00 ,  C12N5/071

CPC classification number: A61L27/3895 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  C12N5/0068 ,  C12N5/0688 ,  C12N2533/30

Abstract: The problem to be solved by the present invention is to provide a cultured cell sheet having a superior tissue adhesiveness and an excellent flexibility. The problems can be solved by producing the cultured cell sheet in which cells are cultured in the presence of a surfactant protein or a cross-linking inhibitor on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; and detaching the cultured cell sheet.