公开(公告)号：US09091632B2

公开(公告)日：2015-07-28

申请号：US14038900

申请日：2013-09-27

申请人： CEM Corp.

发明人： Michael J Collins, Sr. ,  Joseph J Lambert ,  Timothy A Zawatsky ,  David L Herman

IPC分类号： G01N33/00 ,  G01N1/00 ,  G01N21/17 ,  G01N33/68 ,  G01N21/25 ,  G01N1/28 ,  G01N35/10 ,  G01N33/12 ,  G01N1/40

CPC分类号： G01N33/6839 ,  G01N1/286 ,  G01N21/17 ,  G01N21/251 ,  G01N33/12 ,  G01N33/6803 ,  G01N35/1095 ,  G01N2001/4088 ,  Y10T436/10 ,  Y10T436/105831 ,  Y10T436/25

摘要： A dye binding method for protein analysis is disclosed. The method includes the steps of preparing an initial reference dye solution of unknown concentration from an initial reference dye concentrate and creating an electronic signal based upon the absorbance of the initial reference dye solution. Thereafter, an electronic signal is created based upon the absorbance of a dye filtrate solution prepared from the initial reference dye solution and an initial protein sample. The absorbance signals from the reference dye solution and the dye filtrate solution are sent to a processor that compares the respective absorbances and calculates the protein content of the protein sample based upon the difference between the absorbances. An electronic signal is created based upon the absorbance of a successive dye filtrate solution prepared from the reference dye solution and a successive protein sample, and the absorbance signal from the successive sample dye filtrate solution is sent to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate solution.

摘要翻译： 公开了用于蛋白质分析的染料结合方法。 该方法包括以下步骤：从初始参考染料浓缩物制备未知浓度的初始参考染料溶液，并基于初始参考染料溶液的吸光度产生电子信号。 此后，基于由初始参考染料溶液和初始蛋白质样品制备的染料滤液的吸光度，产生电子信号。 将来自参考染料溶液和染料滤液的吸光度信号送至处理器，该处理器根据吸光度之差来比较各吸光度，并计算蛋白质样品的蛋白质含量。 基于由参考染料溶液和连续蛋白质样品制备的连续染料滤液的吸光度产生电子信号，并将来自连续样品染料滤液的溶液的吸光度信号发送到处理器，以计算蛋白质含量 基于初始参考染料溶液的吸光度与连续的染料滤液的吸光度之间的差异的连续样品。

公开(公告)号：US20140024120A1

公开(公告)日：2014-01-23

申请号：US14038900

申请日：2013-09-27

申请人： CEM Corp.

发明人： Michael J Collins, Sr. ,  Joseph J Lambert ,  Timothy A Zawatsky ,  David L Herman

IPC分类号： G01N21/17

CPC分类号： G01N33/6839 ,  G01N1/286 ,  G01N21/17 ,  G01N21/251 ,  G01N33/12 ,  G01N33/6803 ,  G01N35/1095 ,  G01N2001/4088 ,  Y10T436/10 ,  Y10T436/105831 ,  Y10T436/25

摘要： A dye binding method for protein analysis is disclosed. The method includes the steps of preparing an initial reference dye solution of unknown concentration from an initial reference dye concentrate and creating an electronic signal based upon the absorbance of the initial reference dye solution. Thereafter, an electronic signal is created based upon the absorbance of a dye filtrate solution prepared from the initial reference dye solution and an initial protein sample. The absorbance signals from the reference dye solution and the dye filtrate solution are sent to a processor that compares the respective absorbances and calculates the protein content of the protein sample based upon the difference between the absorbances. An electronic signal is created based upon the absorbance of a successive dye filtrate solution prepared from the reference dye solution and a successive protein sample, and the absorbance signal from the successive sample dye filtrate solution is sent to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate solution.

摘要翻译： 公开了用于蛋白质分析的染料结合方法。 该方法包括以下步骤：从初始参考染料浓缩物制备未知浓度的初始参考染料溶液，并基于初始参考染料溶液的吸光度产生电子信号。 此后，基于由初始参考染料溶液和初始蛋白质样品制备的染料滤液的吸光度，产生电子信号。 将来自参考染料溶液和染料滤液的吸光度信号送至处理器，该处理器根据吸光度之差来比较各吸光度，并计算蛋白质样品的蛋白质含量。 基于由参考染料溶液和连续蛋白质样品制备的连续染料滤液的吸光度产生电子信号，并将来自连续样品染料滤液的溶液的吸光度信号发送到处理器，以计算蛋白质含量 基于初始参考染料溶液的吸光度与连续的染料滤液的吸光度之间的差异的连续样品。