公开(公告)号：US11744863B2

公开(公告)日：2023-09-05

申请号：US16622446

申请日：2018-06-15

申请人： CENTRE HOSPITALIER REGIONAL ET UNIVERSITAIRE DE LILLE (CHRU) ,  UNIVERSITE DE LILLE ,  UNIVERSITE DU LITTORAL COTE D'OPALE ,  INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) ,  TAIPEI MEDICAL UNIVERSITY

发明人： David Devos ,  Thierry Burnouf ,  Jean-christophe Devedjian ,  Ming-Li Chou ,  Flore Gouel

IPC分类号： A61K35/19 ,  A61P25/28 ,  A61K9/00 ,  C12N5/078

CPC分类号： A61K35/19 ,  A61K9/0085 ,  A61P25/28 ,  C12N5/0644

摘要： Process for preparing a heat-treated pooled human platelet lysate, said process comprising the steps of: a) Providing a pooled human platelet lysate (p HPL), b) Heat-treating the pooled human platelet lysate at a temperature of 50° C. to 70° C. during 20 to 40 minutes, c) Purifying the heat-treated pooled human platelet lysate of step b).

公开(公告)号：US10787635B2

公开(公告)日：2020-09-29

申请号：US16029680

申请日：2018-07-09

申请人： Taipei Medical University

发明人： Peng-Yuan Wang ,  Yin-Ju Chen ,  Long-Sheng Lu ,  Thierry Burnouf

IPC分类号： C12M1/12 ,  C12N5/09 ,  G01N33/50

摘要： A method for expanding circulating tumor cell in vitro includes preparing a cell culture tool having a multi-particle colloidal crystal layer, preparing a cell solution including circulating tumor cells, and contacting the cell solution with the multi-particle colloidal crystal layer, to attach the circulating tumor cells in the cell solution to the multi-particle colloidal crystal layer and rapidly expand by 20 times or more. The multi-particle colloidal crystal layer at least includes first particles having a particle size of 1000 to 5000 nm and second particles having a particle size of 20 to 400 nm. The culture medium in the cell solution at least includes a platelet lysate.

公开(公告)号：US20190300832A1

公开(公告)日：2019-10-03

申请号：US16029680

申请日：2018-07-09

申请人： Taipei Medical University

发明人： Peng-Yuan Wang ,  Yin-Ju Chen ,  Long-Sheng Lu ,  Thierry Burnouf

IPC分类号： C12M1/12 ,  C12N5/09 ,  G01N33/50

摘要： A method for expanding circulating tumor cell in vitro includes preparing a cell culture tool having a multi-particle colloidal crystal layer, preparing a cell solution including circulating tumor cells, and contacting the cell solution with the multi-particle colloidal crystal layer, to attach the circulating tumor cells in the cell solution to the multi-particle colloidal crystal layer and rapidly expand by 20 times or more. The multi-particle colloidal crystal layer at least includes first particles having a particle size of 1000 to 5000 nm and second particles having a particle size of 20 to 400 nm. The culture medium in the cell solution at least includes a platelet lysate.