公开(公告)号：US11939625B2

公开(公告)日：2024-03-26

申请号：US17258932

申请日：2019-08-24

申请人： CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Zhong Cao ,  Jia Yang ,  Chen Liu ,  Zhongliang Xiao ,  Dan Li ,  Ling Zhang ,  Yuyang Zhang ,  Jiaxin Li

IPC分类号： C12Q1/6809 ,  G01N27/26

CPC分类号： C12Q1/6809 ,  G01N27/26 ,  C12Q2500/00 ,  C12Q2545/10 ,  C12Q2560/00 ,  C12Q2565/607

摘要： A method and a sensor for detecting L-cystine are disclosed. The method is implemented by assembling a sodium 3,3′-dithiodipropane sulfonate (SPS) membrane on a surface of Au membrane layer of an Au electrode and using an extended gate of field effect transistor (FET) and in-situ signal amplification of the FET to detect L-cystine sensitively. The polyanion of the SPS membrane adsorbs and binds a positively charged target L-cystine through electrostatic interaction, thus forming an electric double layer structure to generate a membrane potential identifying a monovalent organic ammonium ion. The sensor includes the FET, wherein a gate-extended gold electrode is arranged on the FET, and the SPS membrane is assembled on the surface of the Au membrane layer of the gate-extended gold electrode. The sensor has an excellent Nernst response to L-cystine.