公开(公告)号：US11939625B2

公开(公告)日：2024-03-26

申请号：US17258932

申请日：2019-08-24

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong Cao ,  Jia Yang ,  Chen Liu ,  Zhongliang Xiao ,  Dan Li ,  Ling Zhang ,  Yuyang Zhang ,  Jiaxin Li

IPC: C12Q1/6809 ,  G01N27/26

CPC classification number: C12Q1/6809 ,  G01N27/26 ,  C12Q2500/00 ,  C12Q2545/10 ,  C12Q2560/00 ,  C12Q2565/607

Abstract: A method and a sensor for detecting L-cystine are disclosed. The method is implemented by assembling a sodium 3,3′-dithiodipropane sulfonate (SPS) membrane on a surface of Au membrane layer of an Au electrode and using an extended gate of field effect transistor (FET) and in-situ signal amplification of the FET to detect L-cystine sensitively. The polyanion of the SPS membrane adsorbs and binds a positively charged target L-cystine through electrostatic interaction, thus forming an electric double layer structure to generate a membrane potential identifying a monovalent organic ammonium ion. The sensor includes the FET, wherein a gate-extended gold electrode is arranged on the FET, and the SPS membrane is assembled on the surface of the Au membrane layer of the gate-extended gold electrode. The sensor has an excellent Nernst response to L-cystine.

公开(公告)号：US11761922B2

公开(公告)日：2023-09-19

申请号：US16969588

申请日：2019-12-31

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong Cao ,  Ningtao Zhou ,  Li Zhou ,  Qin Zhu ,  Jinglin He ,  Zhongliang Xiao ,  Yumin He ,  Zemeng Feng ,  Yulong Yin

IPC: G01N27/327 ,  C07K1/107 ,  G01N27/48

CPC classification number: G01N27/3277 ,  C07K1/1077 ,  G01N27/48

Abstract: A method and a sensor for detecting L-arginine are provided. The method includes synthesizing ferrocene-functionalized hexadecapeptide dithiocyclopentane (FC-P16 Peptide), preparing a polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE), detecting L-Arg and other steps. The results show that the polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE) exhibits excellent electrochemical response properties to L-Arg. In 10 mmol/L phosphate-buffered saline (PBS, pH=7.4), the DPV response peak current of the polypeptide composite membrane-modified electrode has an excellent linear relationship with the L-Arg concentration of 1.0×10−13 mol/L to 1.0×10−7 mol/L, with a detection limit of 1.0×10−13 mol/L. With prominent reproducibility, repeatability and selectivity, the modified electrode has potential application in life science and nutritional health.

公开(公告)号：US11079353B2

公开(公告)日：2021-08-03

申请号：US16955797

申请日：2019-08-24

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong Cao ,  Yuyu Peng ,  Chen Liu ,  Ling Tang ,  Qin Zhu ,  Min Jin ,  Jinglin He ,  Zhongliang Xiao

IPC: G01N27/414

Abstract: A method and a sensor for detecting L-cysteine based on a 3,3′-dithiobis (1-propanesulfonate)-mercury composite membrane are provided. The method includes: implanting a p-well and an N-type substrate on field effect transistor substrate, constructing a source electrode and a drain electrode at the p-well, constructing a silicon dioxide layer on the substrate, plating an aluminum-copper alloy layer, a chromium-palladium alloy layer, and a gold film layer on a polysilicon gate substrate, constructing a silicon nitride layer on the substrate and the silicon dioxide layer; extending a gate part; preparing an ethanol solution of disodium 3,3′-dithiobis (1-propanesulfonate), cleaning and soaking gate gold electrode in the solution, followed by soaking in an ethanol solution of mercuric nitrate, and then washing the gate gold electrode; connecting electrode interfaces, inserting the electrode into PBS, and connecting power interfaces to a stabilized voltage supply, connecting a signal output interface to a multimeter.