公开(公告)号：US20170342115A1

公开(公告)日：2017-11-30

申请号：US15679679

申请日：2017-08-17

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Sol KIM ,  Hye Won KIM ,  Jin Sook CHANG ,  In Hwa YOO

IPC: C07K14/245 ,  C12P13/06 ,  C12P13/00 ,  C12N15/63 ,  C12N15/52 ,  C12N9/16 ,  C12N9/10 ,  C12P13/12 ,  C12N9/04

CPC classification number: C07K14/245 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/16 ,  C12N15/52 ,  C12N15/63 ,  C12P13/005 ,  C12P13/06 ,  C12P13/12

Abstract: The present invention relates to an RhtB (homoserine/homoserine lactone export transporter) protein variant having an enhanced ability to export O-phosphoserine (OPS) that is a precursor of L-cysteine, a polynucleotide encoding the protein, a vector comprising the polynucleotide, an OPS-producing microorganism comprising the protein variant, a method of producing O-phosphoserine using the microorganism, and a method for preparing cysteine or its derivatives, which comprises reacting O-phosphoserine, produced by the method above, with a sulfide in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism that expresses OPSS.

公开(公告)号：US20170342114A1

公开(公告)日：2017-11-30

申请号：US15679671

申请日：2017-08-17

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Sol KIM ,  Hye Won KIM ,  Jin Sook CHANG ,  In Hwa YOO

IPC: C07K14/245 ,  C12P13/06 ,  C12P13/00 ,  C12N15/63 ,  C12N15/52 ,  C12N9/16 ,  C12N9/10 ,  C12P13/12 ,  C12N9/04

CPC classification number: C07K14/245 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/16 ,  C12N15/52 ,  C12N15/63 ,  C12P13/005 ,  C12P13/06 ,  C12P13/12

Abstract: The present invention relates to an RhtB (homoserine/homoserine lactone export transporter) protein variant having an enhanced ability to export O-phosphoserine (OPS) that is a precursor of L-cysteine, a polynucleotide encoding the protein, a vector comprising the polynucleotide, an OPS-producing microorganism comprising the protein variant, a method of producing O-phosphoserine using the microorganism, and a method for preparing cysteine or its derivatives, which comprises reacting O-phosphoserine, produced by the method above, with a sulfide in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism that expresses OPSS.

公开(公告)号：US20190023749A1

公开(公告)日：2019-01-24

申请号：US16156778

申请日：2018-10-10

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Sol KIM ,  Hye Won KIM ,  Jin Sook CHANG ,  In Hwa YOO

IPC: C07K14/245 ,  C12P13/00 ,  C12P13/06 ,  C12N15/63 ,  C12P13/12 ,  C12N9/16 ,  C12N9/10 ,  C12N9/04 ,  C12N15/52

Abstract: The present invention relates to an RhtB (homoserine/homoserine lactone export transporter) protein variant having an enhanced ability to export O-phosphoserine (OPS) that is a precursor of L-cysteine, a polynucleotide encoding the protein, a vector comprising the polynucleotide, an OPS-producing microorganism comprising the protein variant, a method of producing O-phosphoserine using the microorganism, and a method for preparing cysteine or its derivatives, which comprises reacting O-phosphoserine, produced by the method above, with a sulfide in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism that expresses OPSS.

公开(公告)号：US20170073715A1

公开(公告)日：2017-03-16

申请号：US15276661

申请日：2016-09-26

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Jin Sook CHANG ,  Jae Hyun JO ,  Hyun Ae BAE ,  Byeong Cheol SONG ,  Sol KIM ,  Hye Won KIM

IPC: C12P13/12 ,  C12N15/77 ,  C12N15/70 ,  C12N9/16 ,  C12N9/10

CPC classification number: C12P13/12 ,  C12N9/1085 ,  C12N9/16 ,  C12N15/70 ,  C12N15/77 ,  C12P13/06 ,  C12Y205/01065 ,  C12Y301/03003

Abstract: The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having the properties noted above are also provided herein.

Abstract translation: 本发明提供了通过培养具有降低的内源磷酸丝氨酸磷酸酶活性的微生物来制备半胱氨酸或其衍生物的方法。 由这样的生物体产生的O-磷酸丝氨酸然后可以在硫酸酯或表达巯基化酶的微生物的存在下与硫化物反应，以产生半胱氨酸或其衍生物。 具有上述性质的微生物也在本文中提供。

公开(公告)号：US20190338242A1

公开(公告)日：2019-11-07

申请号：US16474291

申请日：2017-12-28

Applicant: CJ Cheiljedang Corporation

Inventor： Se Hoon SIM ,  Seo-Yun KIM ,  Sol KIM ,  Hye Won KIM ,  Inhwa YOO

IPC: C12N1/20 ,  C12P13/06 ,  C12P13/12

Abstract: The present application relates to a microorganism producing O-phosphoserine and a method for producing O-phosphoserine, cysteine or a cysteine derivative using same.

公开(公告)号：US20170260556A1

公开(公告)日：2017-09-14

申请号：US15329921

申请日：2015-08-10

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Sol KIM ,  In Hwa YOO ,  Jin Sook CHANG ,  Hye Won KIM

IPC: C12P13/12 ,  C12P13/06 ,  C12N9/10 ,  C12N9/16 ,  C12N9/04

CPC classification number: C12P13/12 ,  C12N9/0006 ,  C12N9/1085 ,  C12N9/1096 ,  C12N9/16 ,  C12P13/06 ,  C12Y101/01095 ,  C12Y205/01065 ,  C12Y206/01052 ,  C12Y301/03003

Abstract: The present invention relates to a microorganism, wherein the activity of a polypeptide capable of exporting O-phosphoserine (OPS) is enhanced, and a method of producing O-phosphoserine, cysteine, or a cysteine derivative using the microorganism.

公开(公告)号：US20160130310A1

公开(公告)日：2016-05-12

申请号：US14890422

申请日：2014-05-09

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Sol KIM ,  Hye Won KIM ,  Jin Sook CHANG ,  In Hwa YOO

IPC: C07K14/245 ,  C12P13/12 ,  C12P13/00

CPC classification number: C07K14/245 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/16 ,  C12N15/52 ,  C12N15/63 ,  C12P13/005 ,  C12P13/06 ,  C12P13/12

Abstract: The present invention relates to an RhtB (homoserine/homoserine lactone export transporter) protein variant having an enhanced ability to export O-phosphoserine (OPS) that is a precursor of L-cysteine, a polynucleotide encoding the protein, a vector comprising the polynucleotide, an OPS-producing microorganism comprising the protein variant, a method of producing O-phosphoserine using the microorganism, and a method for preparing cysteine or its derivatives, which comprises reacting O-phosphoserine, produced by the method above, with a sulfide in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism that expresses OPSS.

Abstract translation: 本发明涉及具有增强作为L-半胱氨酸前体的O-磷酸丝氨酸（OPS）的能力的RhtB（高丝氨酸/高丝氨酸内酯输出转运蛋白）蛋白质，编码该蛋白质的多核苷酸，包含多核苷酸的载体， 包含蛋白质变体的OPS生产微生物，使用该微生物生产O-磷酸丝氨酸的方法，以及制备半胱氨酸或其衍生物的方法，其包括使上述方法制备的O-磷酸丝氨酸与硫化物在存在下反应 的O-磷酸丝氨酸巯基化酶（OPSS）或表达OPSS的微生物。