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公开(公告)号：US09902753B2
公开(公告)日：2018-02-27
申请号：US14406192
申请日：2013-06-05
发明人： Gil Bu Kang , Chung Min Lee , Jee Won Ahn , Dong Eok Lee , Chang Bong Jun , Won Jeong Lee , Sung Yoo Cho , Chi Hye Park , Ki Wan Kim , Yoon Jung Lee , Ji Hyun Moon , Hoo Keun Oh , Young Joon Park , Sang Hyun Lee , Hyoung Taek Lim , Soon Jae Park , Hye Shin Chung , Sang Mee Lee
CPC分类号： C07K1/16 , C07K1/14 , C07K14/61 , C07K14/8125 , C07K19/00 , C07K2319/00
摘要： The present invention relates to a long-acting human growth hormone NexP-hGH protein and its production method. More specifically, it relates to a specific isoform of long-acting human growth hormone NexP-hGH protein in which human growth hormone is fused with a highly glycosylated alpha-1 antitrypsin mutant whereby long-acting properties in vivo are increased. The present invention also relates to a high-purity purification method for NexP-hGH, which includes the steps of: (a) carrying out anion-exchange resin chromatography on a biological emulsion comprising NexP-hGH in which human growth hormone is fused with an alpha-1 antitrypsin mutant; (b) carrying out hydrophobic resin chromatography on the biological emulsion comprising NexP-hGH, or on the eluate produced in step (a); and (c) carrying out affinity chromatography, entailing packing with a resin to which anti-alpha-1 antitrypsin antibody fragments are attached, on the biological emulsion comprising NexP-hGH and on the eluate produced in step (a) or step (b).
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2.发明申请Highly Glycosylated Long-Acting Human Growth Hormone Protein and Production Method for Same 有权高度糖基化的长效人类生长激素蛋白及其生产方法公开(公告)号：US20150126715A1
公开(公告)日：2015-05-07
申请号：US14406192
申请日：2013-06-05
发明人： Gil Bu Kang , Chung Min Lee , Jee Won Ahn , Dong Eok Lee , Chang Bong Jun , Won Jeong Lee , Sung Yoo Cho , Chi Hye Park , Ki Wan Kim , Yoon Jung Lee , Ji Hyun Moon , Hoo Keun Oh , Young Joon Park , Sang Hyun Lee , Hyoung Taek Lim , Soon Jae Park , Hye Shin Chung , Sang Mee Lee
CPC分类号： C07K1/16 , C07K1/14 , C07K14/61 , C07K14/8125 , C07K19/00 , C07K2319/00
摘要： The present invention relates to a long-acting human growth hormone NexP-hGH protein and its production method. More specifically, it relates to a specific isoform of long-acting human growth hormone NexP-hGH protein in which human growth hormone is fused with a highly glycosylated alpha-1 antitrypsin mutant whereby long-acting properties in vivo are increased. The present invention also relates to a high-purity purification method for NexP-hGH, which includes the steps of: (a) carrying out anion-exchange resin chromatography on a biological emulsion comprising NexP-hGH in which human growth hormone is fused with an alpha-1 antitrypsin mutant; (b) carrying out hydrophobic resin chromatography on the biological emulsion comprising NexP-hGH, or on the eluate produced in step (a); and (c) carrying out affinity chromatography, entailing packing with a resin to which anti-alpha-1 antitrypsin antibody fragments are attached, on the biological emulsion comprising NexP-hGH and on the eluate produced in step (a) or step (b).
摘要翻译： 本发明涉及长效人生长激素NexP-hGH蛋白及其制备方法。 更具体地说，它涉及长效人生长激素NexP-hGH蛋白的特异性同种型，其中人生长激素与高度糖基化的α-1抗胰蛋白酶突变体融合，由此体内长效特性增加。 本发明还涉及一种用于NexP-hGH的高纯度纯化方法，其包括以下步骤：（a）在包含NexP-hGH的生物乳液上进行阴离子交换树脂色谱法，其中人生长激素与 α-1抗胰蛋白酶突变体; （b）对包含NexP-hGH的生物乳液或在步骤（a）中产生的洗脱液上进行疏水性树脂色谱法; 和（c）进行亲和层析，使包含与抗-α-1抗胰蛋白酶抗体片段相连的树脂包含在包含NexP-hGH的生物乳液和步骤（a）或步骤（b）中产生的洗脱液上） 。
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公开(公告)号：US11414476B2
公开(公告)日：2022-08-16
申请号：US15767806
申请日：2016-10-14
申请人： ALTEOGEN, INC.
发明人： Soon Jae Park , Hye Shin Chung , Sun Ah You , Jeong Soo Cho
摘要： The present invention relates to a method for preparing a fusion protein having an IgG Fc domain and, specifically, to a method for preparing a fusion protein having an IgG Fc domain, the method additionally comprising a step of culturing cells, which produce the fusion protein, at a decreased culture temperature, thereby increasing cell growth and cell viability so as to increase fusion protein productivity and inhibiting aggregate generation so as to improve quality and production yield.
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