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公开(公告)号：US09902753B2
公开(公告)日：2018-02-27
申请号：US14406192
申请日：2013-06-05
发明人： Gil Bu Kang , Chung Min Lee , Jee Won Ahn , Dong Eok Lee , Chang Bong Jun , Won Jeong Lee , Sung Yoo Cho , Chi Hye Park , Ki Wan Kim , Yoon Jung Lee , Ji Hyun Moon , Hoo Keun Oh , Young Joon Park , Sang Hyun Lee , Hyoung Taek Lim , Soon Jae Park , Hye Shin Chung , Sang Mee Lee
CPC分类号： C07K1/16 , C07K1/14 , C07K14/61 , C07K14/8125 , C07K19/00 , C07K2319/00
摘要： The present invention relates to a long-acting human growth hormone NexP-hGH protein and its production method. More specifically, it relates to a specific isoform of long-acting human growth hormone NexP-hGH protein in which human growth hormone is fused with a highly glycosylated alpha-1 antitrypsin mutant whereby long-acting properties in vivo are increased. The present invention also relates to a high-purity purification method for NexP-hGH, which includes the steps of: (a) carrying out anion-exchange resin chromatography on a biological emulsion comprising NexP-hGH in which human growth hormone is fused with an alpha-1 antitrypsin mutant; (b) carrying out hydrophobic resin chromatography on the biological emulsion comprising NexP-hGH, or on the eluate produced in step (a); and (c) carrying out affinity chromatography, entailing packing with a resin to which anti-alpha-1 antitrypsin antibody fragments are attached, on the biological emulsion comprising NexP-hGH and on the eluate produced in step (a) or step (b).
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2.发明申请Highly Glycosylated Long-Acting Human Growth Hormone Protein and Production Method for Same 有权高度糖基化的长效人类生长激素蛋白及其生产方法公开(公告)号：US20150126715A1
公开(公告)日：2015-05-07
申请号：US14406192
申请日：2013-06-05
发明人： Gil Bu Kang , Chung Min Lee , Jee Won Ahn , Dong Eok Lee , Chang Bong Jun , Won Jeong Lee , Sung Yoo Cho , Chi Hye Park , Ki Wan Kim , Yoon Jung Lee , Ji Hyun Moon , Hoo Keun Oh , Young Joon Park , Sang Hyun Lee , Hyoung Taek Lim , Soon Jae Park , Hye Shin Chung , Sang Mee Lee
CPC分类号： C07K1/16 , C07K1/14 , C07K14/61 , C07K14/8125 , C07K19/00 , C07K2319/00
摘要： The present invention relates to a long-acting human growth hormone NexP-hGH protein and its production method. More specifically, it relates to a specific isoform of long-acting human growth hormone NexP-hGH protein in which human growth hormone is fused with a highly glycosylated alpha-1 antitrypsin mutant whereby long-acting properties in vivo are increased. The present invention also relates to a high-purity purification method for NexP-hGH, which includes the steps of: (a) carrying out anion-exchange resin chromatography on a biological emulsion comprising NexP-hGH in which human growth hormone is fused with an alpha-1 antitrypsin mutant; (b) carrying out hydrophobic resin chromatography on the biological emulsion comprising NexP-hGH, or on the eluate produced in step (a); and (c) carrying out affinity chromatography, entailing packing with a resin to which anti-alpha-1 antitrypsin antibody fragments are attached, on the biological emulsion comprising NexP-hGH and on the eluate produced in step (a) or step (b).
摘要翻译： 本发明涉及长效人生长激素NexP-hGH蛋白及其制备方法。 更具体地说，它涉及长效人生长激素NexP-hGH蛋白的特异性同种型，其中人生长激素与高度糖基化的α-1抗胰蛋白酶突变体融合，由此体内长效特性增加。 本发明还涉及一种用于NexP-hGH的高纯度纯化方法，其包括以下步骤：（a）在包含NexP-hGH的生物乳液上进行阴离子交换树脂色谱法，其中人生长激素与 α-1抗胰蛋白酶突变体; （b）对包含NexP-hGH的生物乳液或在步骤（a）中产生的洗脱液上进行疏水性树脂色谱法; 和（c）进行亲和层析，使包含与抗-α-1抗胰蛋白酶抗体片段相连的树脂包含在包含NexP-hGH的生物乳液和步骤（a）或步骤（b）中产生的洗脱液上） 。
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公开(公告)号：US11866492B2
公开(公告)日：2024-01-09
申请号：US16980074
申请日：2019-03-13
申请人： ALTEOGEN, INC.
发明人： Soon Jae Park , Hye-Shin Chung , Sunbae Lee
IPC分类号： C07K16/28 , A61K47/68 , G01N33/574 , C07K16/02
CPC分类号： C07K16/02 , A61K47/6849 , G01N33/57492 , C07K2317/732 , C07K2317/76 , C07K2317/92 , G01N2333/705
摘要： A modified antibody that binds specifically to folate receptor alpha (FOLR1) and blocks the activity of FOLR1 with an increased binding affinity, an antigen-binding fragment thereof, a composition containing the antibody or fragment, and their uses are disclosed. The modified antibody or antigen-binding fragment thereof may be used for the prevention or treatment of cancer proliferative disorder associated with an increased FOR1 expression, and may also be used for diagnosis of the disease. The proliferative disorder may be cancer.
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公开(公告)号：US09982032B2
公开(公告)日：2018-05-29
申请号：US15190378
申请日：2016-06-23
申请人： Alteogen, Inc.
发明人： Soon Jae Park , Hye-Shin Chung , Jin Hwan Kim , Yong Mo Kim , Jun Young Kim
CPC分类号： C07K14/71 , A61K9/0019 , A61K38/179 , A61K47/183 , A61K47/26 , C07K2319/30
摘要： A stable liquid formulation includes a fusion protein having an Fc domain of a human immunoglobulin G (IgG), in particular, a protein in which an Fc domain of a human immunoglobulin G (IgG) and a soluble extracellular domain of a vascular endothelial growth factor (VEGF) receptor are fused (e.g., aflibercept)). A composition for stabilizing a protein and a method for stabilizing a protein in which an Fc domain of an IgG and a soluble extracellular domain of a VEGF receptor are fused are disclosed. The present invention improves therapeutic effects on various ophthalmic diseases (e.g., retinal vein occlusion, diabetic macular edema, choroidal neovascularization and wet age-related macular degeneration, etc.) caused by abnormal angiogenesis, while pursuing stabilization of bioactivity through a stable liquid formulation suitable for intravitreal injection of an anti-VEGF-Fc fusion protein including aflibercept.
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5.发明申请COMPOSITION FOR STABILIZING FUSION PROTEIN IN WHICH PROTEIN AND FC DOMAIN ARE FUSED 有权用于稳定蛋白质和FC域的融合蛋白的组合物被融合公开(公告)号：US20150313996A1
公开(公告)日：2015-11-05
申请号：US14439644
申请日：2013-10-23
申请人： ALTEOGEN INC.
发明人： Soon Jae Park , Seong-Kyu Kim
IPC分类号： A61K47/02 , C01C1/16 , C09K15/20 , C07K14/705
CPC分类号： A61K47/02 , A61K9/08 , A61K38/1793 , A61K47/26 , C01C1/164 , C07K14/70578 , C07K2319/30 , C09K15/20
摘要： The present invention relates to a composition for stabilizing a fusion protein of a physiologically active protein and an Fc domain, and more particularly to a method of stabilizing a fusion protein of a protein and an Fc domain using a composition containing an ammonium salt or a combination of an ammonium salt and succinate. The composition containing an ammonium salt or a combination of an ammonium salt and succinate according to the present invention can effectively inhibit the aggregation of the protein-Fc domain fusion protein, and thus enables the fusion protein to be stored for a long period of time. Accordingly, the composition can be widely used in the medical field that uses the protein-Fc domain fusion protein.
摘要翻译： 本发明涉及用于稳定生理活性蛋白质和Fc结构域的融合蛋白质的组合物，更具体地涉及使用含有铵盐或组合物的组合物来稳定蛋白质和Fc结构域的融合蛋白的方法 的铵盐和琥珀酸盐。 含有本发明铵盐或铵盐和琥珀酸盐的组合的组合物能够有效地抑制蛋白质-Fc结构域融合蛋白质的聚集，能够使融合蛋白质长时间保存。 因此，该组合物可广泛用于使用蛋白质 - Fc结构域融合蛋白的医疗领域。
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公开(公告)号：US09821059B2
公开(公告)日：2017-11-21
申请号：US14884312
申请日：2015-10-15
申请人： Alteogen Inc.
发明人： Soon Jae Park , Hye-Shin Chung , Jin Hwan Kim , Minsoo Byun , Ji Hyeon Yeon
IPC分类号： A61K39/395 , C07K16/24 , A61K47/26 , A61K47/18 , A61K9/00
CPC分类号： A61K39/39591 , A61K9/0019 , A61K47/183 , A61K47/26 , C07K16/241 , C07K2317/21 , C07K2317/76
摘要： The present invention relates to a composition for stabilizing TNFα-binding protein exhibiting physiological activity, and more specifically, to a composition for stabilizing protein including basic amino acid and sugar and/or ammonium salt, a pharmaceutical formulation including the same, and a method for stabilizing TNFα-binding protein. The formulation including basic amino acid; and sugar and/or ammonium salt according to the present invention effectively inhibits aggregation, denaturation and oxidation of TNFα-binding protein used for treating various diseases, for example, an anti-TNF-alpha antibody, such that the protein is capable of being preserved and stored for a long time, which is widely usable and effective in a medical field using TNFα-binding protein, for example, an anti-TNF-alpha antibody.
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公开(公告)号：US20170119903A1
公开(公告)日：2017-05-04
申请号：US15393365
申请日：2016-12-29
申请人： Alteogen Inc.
发明人： Soon Jae Park , Hye-Shin Chung , Seonhun Kwon , Sunbae Lee , Sun-Ah You , Yong Mo Kim
IPC分类号： C07K16/00 , A61K31/704 , C07K16/30 , C07K16/32 , A61K38/07
CPC分类号： A61K47/6855 , A61K31/704 , A61K38/07 , A61K47/65 , A61K47/6803 , A61K47/6809 , A61K47/6817 , A61K47/6849 , A61K47/6851 , A61K47/6871 , C07K16/00 , C07K16/30 , C07K16/3015 , C07K16/32 , C07K2317/24 , C07K2317/52 , C07K2317/73 , C07K2317/76
摘要： The present invention relates to an antibody in which a motif composed of an amino acid or peptide sequence including one or more cysteine residues is bound to the terminus of a parent antibody, particularly the terminus of the heavy chain of the parent antibody. Also, the present invention relates to a modified antibody-drug conjugate (mADC) comprising a drug bound to the antibody, and a method for producing the antibody or the modified antibody-drug conjugate. The modified antibody-drug conjugate according to the invention can accurately deliver the drug to a target cell due its high specificity to antigen, and thus can increase the therapeutic effect of the drug. Also, it can increase the usability of drugs, particularly anticancer drugs, the use of which is restricted due to their toxicity, despite their high efficacy. Moreover, the invention relates to a composition for treatment of diseases, particularly cancers, which comprise the modified antibody-drug conjugate.
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8.发明申请COMPOSITION FOR STABILIZING PROTEIN AND PHARMACEUTICAL FORMULATION COMPRISING THE SAME 有权用于稳定蛋白质的组合物和包含该蛋白质的药物制剂公开(公告)号：US20160114036A1
公开(公告)日：2016-04-28
申请号：US14884312
申请日：2015-10-15
申请人： Alteogen Inc.
发明人： Soon Jae Park , Hye-Shin Chung , Jin Hwan Kim , Minsoo Byun , Ji Hyeon Yeon
CPC分类号： A61K39/39591 , A61K9/0019 , A61K47/183 , A61K47/26 , C07K16/241 , C07K2317/21 , C07K2317/76
摘要： The present invention relates to a composition for stabilizing TNFα-binding protein exhibiting physiological activity, and more specifically, to a composition for stabilizing protein including basic amino acid and sugar and/or ammonium salt, a pharmaceutical formulation including the same, and a method for stabilizing TNFα-binding protein. The formulation including basic amino acid; and sugar and/or ammonium salt according to the present invention effectively inhibits aggregation, denaturation and oxidation of TNFα-binding protein used for treating various diseases, for example, an anti-TNF-alpha antibody, such that the protein is capable of being preserved and stored for a long time, which is widely usable and effective in a medical field using TNFα-binding protein, for example, an anti-TNF-alpha antibody.
摘要翻译： 本发明涉及用于稳定表现出生理活性的TNFα结合蛋白的组合物，更具体地，涉及用于稳定包括碱性氨基酸和糖和/或铵盐的蛋白质的组合物，包含其的药物制剂及其制备方法 稳定TNFα结合蛋白。 该制剂包括碱性氨基酸; 和根据本发明的糖和/或铵盐有效抑制用于治疗各种疾病的TNFα结合蛋白的聚集，变性和氧化，例如抗TNF-α抗体，使得蛋白质能够被保留 并存储长时间，其在使用TNFα结合蛋白的医疗领域中广泛可用和有效，例如抗TNF-α抗体。
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公开(公告)号：US12090210B2
公开(公告)日：2024-09-17
申请号：US17551103
申请日：2021-12-14
申请人： ALTEOGEN, INC.
发明人： Soon Jae Park , Hye-Shin Chung , Sunbae Lee , Minsoo Byun
CPC分类号： A61K47/6803 , A61K47/65 , A61P35/00 , C07K16/18 , A61K45/06
摘要： The present disclosure relates to an antibody-drug conjugate in which a modified antibody comprising a motif having a specific structure at the end of the antibody is conjugated to a drug via a linker, and a composition comprising the same, and more particularly to a modified antibody-drug conjugate (mADC) comprising a modified antibody that has a significantly increased conjugation yield of drug due to a motif bound to the heavy chain or light chain C-terminus of the antibody, and to a composition comprising the same.
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公开(公告)号：US20200254111A1
公开(公告)日：2020-08-13
申请号：US16088804
申请日：2017-03-30
申请人： ALTEOGEN, INC.
发明人： Soon Jae Park , Hye-Shin Chung , Sunbae Lee , Minsoo Byun
摘要： The present disclosure relates to an antibody-drug conjugate in which a modified antibody comprising a motif having a specific structure at the end of the antibody is conjugated to a drug via a linker, and a composition comprising the same, and more particularly to a modified antibody-drug conjugate (mADC) comprising a modified antibody that has a significantly increased conjugation yield of drug due to a motif bound to the heavy chain or light chain C-terminus of the antibody, and to a composition comprising the same.
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