公开(公告)号：US20220380716A1

公开(公告)日：2022-12-01

申请号：US17642027

申请日：2020-09-11

Applicant: CORNING INCORPORATED

Inventor： Li Cao ,  Kirsten Lee Cooper ,  Feng Li ,  Sweta Nimesh Parikh ,  Rongjun Zuo

IPC: C12M1/00 ,  C12M1/32 ,  C12M1/12 ,  B65D81/18 ,  B65D81/38 ,  B65D81/02

Abstract: A kit for temperature maintenance and shipment integrity of a cell culture is provided, including a well plate, a cell culture, culture media, an insulated receptacle, and a temperature maintenance element. The kit may include a heat release element. The well plate includes a plurality of wells. The cell culture is arranged within each of the plurality of wells of the well plate. Culture media is arranged within each of the plurality of wells of the well plate, where the culture media encapsulates the cell culture. The well plate is arranged within the insulated receptacle. The temperature maintenance element is arranged within the insulated receptacle. The heat release element is also arranged within the insulated receptacle.

公开(公告)号：US20220259540A1

公开(公告)日：2022-08-18

申请号：US17734660

申请日：2022-05-02

Applicant: CORNING INCORPORATED

Inventor： Feng Li ,  Allison Jean Tanner

IPC: C12M1/12 ,  C12M1/32 ,  C12M1/00 ,  C12N5/00

Abstract: A cell culture well insert may include a frame defining a first open end, a second open end, and at least one support extending therebetween. The cell culture well insert may also include a fluid permeable mesh coupled to the frame and disposed across the second open end. The mesh may define pores that have an average pore size in a range from 10 micrometers to 100 micrometers.

公开(公告)号：US20230357688A1

公开(公告)日：2023-11-09

申请号：US18028403

申请日：2021-09-29

Applicant: CORNING INCORPORATED

Inventor： Feng Li ,  Gregory Roger Martin

IPC: C12M1/32 ,  C12M1/12 ,  C12M3/00

CPC classification number: C12M23/12 ,  C12M25/14 ,  C12M25/10 ,  C12M21/08

Abstract: A cell culture device comprises a multi-well cell culture plate comprising a plurality of wells, each well comprising a top, a bottom, and a sidewall disposed between the top and the bottom and having an interior surface comprising an ultra-low attachment surface. A plurality of scaffolds are disposed within wells of the multi-well cell culture plate, each scaffold comprising a cell-adherent surface. In some embodiments, the scaffold comprises a fiber scaffold. In some embodiments, the scaffold comprises an artificial vascular scaffold. In some embodiments, cell culture devices comprise a plurality of hydrogel scaffolds disposed in a multi-well cell culture plate, the plurality of hydrogel scaffolds comprising hydrogel fibers of differing lengths, wherein opposite ends of a hydrogel fiber are disposed in different wells within the multi-well cell culture plate to create interconnected wells.

公开(公告)号：US20210018492A1

公开(公告)日：2021-01-21

申请号：US16980189

申请日：2019-03-12

Applicant: CORNING INCORPORATED

Inventor： Vasiliy Nikolaevich Goral ,  Feng Li ,  Gregory Roger Martin ,  Allison Jean Tanner ,  Rongjun Zuo

IPC: G01N33/50 ,  C12M1/00 ,  C12M1/32 ,  C12M1/04

Abstract: The present disclosure relates to methods for evaluating the interaction of a candidate compound on 3D hepatocyte spheroid in an invitro culture, including evaluating the metabolism of a candidate compound, for use in various biochemical and molecular biology studies. The methods are performed in labware that combine 3D spheroid culture with micro-patterned design that allows for multiple to several hundreds of spheroids to be treated under the same conditions and to produce sufficient materials (e.g., parent drug, drug metabolites, DNA, RNA, and proteins from cells) and higher detection signal intensity for ADME/Tox (absorption, distribution, metabolism, excretion and toxicity) studies. The methods allow for, among other uses, the investigation and generation of accurate invitro intrinsic clearance data and thus more accurate prediction of in vivo clearance, particularly with low clearance compounds.