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公开(公告)号：US12116556B2
公开(公告)日：2024-10-15
申请号：US18382827
申请日：2023-10-23
申请人： CORNING INCORPORATED
摘要： A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.
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公开(公告)号：US20240043781A1
公开(公告)日：2024-02-08
申请号：US18382827
申请日：2023-10-23
申请人： CORNING INCORPORATED
摘要： A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.
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公开(公告)号：US20230235262A1
公开(公告)日：2023-07-27
申请号：US18130668
申请日：2023-04-04
申请人： CORNING INCORPORATED
CPC分类号： C12M25/14 , C12M23/02 , C12M23/44 , C12M25/04 , C12M29/10 , C12M41/26 , C12M23/20 , C12M29/26 , C12N5/0068
摘要： A bioreactor system is provided that includes a cell culture vessel having a first end, a second end, and at least one reservoir between the first and second ends; and a cell culture matrix disposed in the at least one reservoir. The cell culture matrix has a structurally defined substrate with a surface for adhering cells thereto. The bioreactor system flows material through the at least one reservoir and through the cell culture matrix in a flow direction from the first end to the second end, and the cell culture matrix exhibits isotropic fluid flow permeability therethrough.
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公开(公告)号：US11692161B2
公开(公告)日：2023-07-04
申请号：US16781807
申请日：2020-02-04
申请人： CORNING INCORPORATED
CPC分类号： C12M25/14 , C12M23/02 , C12M23/20 , C12M23/44 , C12M25/04 , C12M29/10 , C12M29/26 , C12M41/26 , C12M25/02 , C12N5/0068 , C12N5/0602 , C12N2513/00 , C12N2533/30 , C12N2533/40 , C12N2533/90 , C12N2535/00
摘要： A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
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公开(公告)号：US20230012125A1
公开(公告)日：2023-01-12
申请号：US17780448
申请日：2020-11-09
申请人： CORNING INCORPORATED
发明人： John Wilfred Coddaire , Maryanne De Chambeau , James Thomas Eickmann , Paula Mary Flaherty , Anthony Glenn Frutos , Vasiliy Nikolaevich Goral , Angela Langer Julien , Marshall Jay Kosovsky , Brent Ravaughn Lanterman , Gregory Roger Martin , Christie Leigh McCarthy , John Shyu , Tora Ann-Beatrice Eline Sirkka , Allison Jean Tanner , Kimberly Ann Titus , Timothy James Wood
摘要： A sensing cell culture vessel having one or more sensors on or in the vessel is configured to collect readings of various parameters or characteristics of a cell culture located within the sensing cell culture vessel and transmit the readings. The sensing cell culture vessels may be accompanied by a sensing plate having means for reading the one or more sensors and transmitting the one or more sensors to a server hosting electronic lab notebook for analyzing and storage of the readings. Sensing plates may further be equipped with cameras for imaging cell cultures located in the sensing cell culture vessels and transmitting the images to the server hosting the electronic lab notebook for analyzing and storage of the images. Embodiments of the invention allow for the continuous and automatic monitoring of cell cultures.
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公开(公告)号：US20230009199A1
公开(公告)日：2023-01-12
申请号：US17780067
申请日：2020-11-04
申请人： CORNING INCORPORATED
摘要： A modular and stacked cell culture system that includes a standalone cell culture subunit with an interior cavity to house a cell culture substrate in a cell culture space, a fluid inlet to supply fluid to the cell culture space, and a fluid outlet to remove fluid from the cavity. The cavity is arranged for fluid to flow in from the fluid inlet, then through the cell culture space, and then out through the fluid outlet. The subunit further includes an alignment feature on at least one of a top and a bottom of the standalone cell culture subunit, wherein the alignment feature aligns with an alignment feature of another standalone cell culture subunit, such that multiple standalone cell culture subunits are stackable.
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公开(公告)号：US11111470B2
公开(公告)日：2021-09-07
申请号：US17039218
申请日：2020-09-30
申请人： CORNING INCORPORATED
摘要： A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.
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公开(公告)号：US11746322B2
公开(公告)日：2023-09-05
申请号：US17022764
申请日：2020-09-16
申请人： CORNING INCORPORATED
发明人： Ye Fang , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , David Henry , Martial Hervy , Corinne Walerack , Yue Zhou
IPC分类号： C12N5/00 , C08J3/24 , C09D167/00
CPC分类号： C12N5/0075 , C08J3/24 , C09D167/00 , C12N5/0068 , C12N2533/32 , C12N2533/40 , C12N2533/50 , C12N2533/74 , C12N2537/10
摘要： A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.
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公开(公告)号：US20230007854A1
公开(公告)日：2023-01-12
申请号：US17780352
申请日：2020-11-09
申请人： CORNING INCORPORATED
发明人： John Wilfred Coddaire , Maryanne De Chambeau , James Thomas Eickmann , Paula Mary Flaherty , Anthony Glenn Frutos , Vasiliy Nikolaevich Goral , Angela Langer Julien , Marshall Jay Kosovsky , Brent Ravaughn Lanterman , Gregory Roger Martin , Christie Leigh McCarthy , John Forrest Roush , John Shyu , Tora Ann-Beatrice Eline Sirkka , Allison Jean Tanner , Kimberly Ann Titus , Todd Michael Upton , Timothy James Wood
摘要： A connected ecosystem for a laboratory environment comprises an electronic lab notebook, and instrumented biosafety cabinet, and one or more sensing vessels containing cell cultures. The electronic lab notebook interfaces with the instrumented biosafety cabinet to provide instructions, guidance, and monitoring of a user during the set up of the experimental protocol and to receive commands from the user via one of several input modalities. After the experimental protocol has been set up in the instrumented biosafety cabinet, cell cultures may be moved to an incubator where the connected ecosystem may provide automatic monitoring of the cultures. The automatic monitoring is provided by sensors integrated into cell culture vessels and supplemented by images of the cell cultures captured by a camera. The user may be informed of deviations from expected results detected based on the automatic monitoring.
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公开(公告)号：US20220364032A1
公开(公告)日：2022-11-17
申请号：US17869419
申请日：2022-07-20
申请人： CORNING INCORPORATED
摘要： A cell culture matrix for a perfusion-flow fixed-bed reactor is provided. The cell culture matrix includes a substrate having a porous sheet for adhering cells thereto. The sheet has a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate, arrayed in a regular pattern, and passing through the thickness of the substrate. The porous sheet is wound into a cylindrical shape having a plurality of wound layers, and the cell culture matrix does not include a spacer material or physical barrier between the plurality of wound layers of the substrate.
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