公开(公告)号：US20230151440A1

公开(公告)日：2023-05-18

申请号：US17810696

申请日：2022-07-05

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/703 ,  C12Q1/68 ,  C12Q1/6851 ,  Y10T436/143333

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US11414716B2

公开(公告)日：2022-08-16

申请号：US16827590

申请日：2020-03-23

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US10889863B2

公开(公告)日：2021-01-12

申请号：US16102583

申请日：2018-08-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/68 ,  C12Q1/70 ,  C12Q1/6883 ,  C12Q1/6851

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US09524900B2

公开(公告)日：2016-12-20

申请号：US14186839

申请日：2014-02-21

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Sameer Walavalkar ,  Mark D. Goldberg ,  Axel Scherer

IPC: H01L21/00 ,  H01L21/762

CPC classification number: H01L21/76283 ,  H01L21/762

Abstract: Novel methods to fabricate biological sensors and electronics are disclosed. A silicon-on-insulator wafer can be employed by etching a pattern of holes in the silicon layer, then a pattern of cavities in the insulating layer, and then sealing the top of the cavities. Further, n or p doped regions and metallic regions can be defined in the processed wafer, thereby enabling integration of biological sensing and electronic capabilities in the same wafer.

Abstract translation: 公开了制造生物传感器和电子学的新方法。 可以通过蚀刻硅层中的孔的图案，然后在绝缘层中蚀刻空腔的图案，然后密封空腔的顶部来采用绝缘体上硅晶片。 此外，可以在经处理的晶片中限定n或p个掺杂区域和金属区域，从而使生物感测和电子能力能够集成在相同的晶片中。

公开(公告)号：US20240167092A1

公开(公告)日：2024-05-23

申请号：US18128764

申请日：2023-03-30

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/70

CPC classification number: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10T436/143333

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Forster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US10597737B2

公开(公告)日：2020-03-24

申请号：US16109288

申请日：2018-08-22

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Methods and kits for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. At least one of the primers is configured to hybridize to a region of the polynucleotide analyte encoding the genetic variation. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US10081844B2

公开(公告)日：2018-09-25

申请号：US15675048

申请日：2017-08-11

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/6851 ,  C12Q1/68

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: Methods of detecting at least one genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, the first primer and the second primer are specific for the polynucleotide analyte. A signal generated by the fluorophore and quencher is measured. PCR is performed with the first primer and the second primer using the polynucleotide analyte as a template, thereby amplifying the template. A signal generated by the fluorophore and quencher from the PCR amplification product is measured. Comparison is made of the signals; and a determination is made of the presence or absence of the at least one genetic variation based i) on the change in signal as determined; and ii) by comparing said change to the change in signal observed upon PCR amplification for a corresponding polynucleotide analyte lacking the at least one genetic variation.

公开(公告)号：US20180030551A1

公开(公告)日：2018-02-01

申请号：US15675048

申请日：2017-08-11

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.

公开(公告)号：US11879162B2

公开(公告)日：2024-01-23

申请号：US17810696

申请日：2022-07-05

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q1/68 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2565/101

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US20210363581A1

公开(公告)日：2021-11-25

申请号：US17125870

申请日：2020-12-17

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/70

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.