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    Sequencing chicken antibody repertoires following hyperimmunization and the identification of antigen-specific monoclonal antibodies
    1.
    发明授权
    Sequencing chicken antibody repertoires following hyperimmunization and the identification of antigen-specific monoclonal antibodies 有权

    公开(公告)号：US11365238B2

    公开(公告)日：2022-06-21

    申请号：US16060304

    申请日：2016-12-07

    申请人： Camas Incorporated

    发明人： Jeff Fagerlund ,  Bradley M. Mitteness ,  Connie Phillips

    IPC分类号： C07K16/00 ,  C07K16/12

    摘要： Neutralizing antibodies through recombinant protein expression were identified by mining antibody repertoires by high-throughput second generation sequencing. Sequencing across the majority of light and heavy chain variable regions of chicken immunoglobulins was performed at two immunological time points: a non-immune (naive) state and a post-hyperimmunization state. The mRNA was extracted from unsorted and unselected PBMC populations and identification of antigen-specific antibody sequences leveraged the significant disparity of abundance of an individual B cell clone in non-immune and immunized states. Through bioinformatic analysis, candidate amino acid sequences for variable heavy chain and light chains were identified. Recombinant polypeptides with the binding domains having the identified amino acid sequences for variable heavy chain and light chains were expressed and screened using immunoassays to confirm antigen-specificity.

    Methods and compositions for reducing microorganisms in the oropharynx, nasopharynx and oral cavities
    4.
    发明申请
    Methods and compositions for reducing microorganisms in the oropharynx, nasopharynx and oral cavities 有权
    用于减少口咽，鼻咽和口腔微生物的方法和组合物

    公开(公告)号：US20140271501A1

    公开(公告)日：2014-09-18

    申请号：US14211947

    申请日：2014-03-14

    申请人： Camas Incorporated

    发明人： Bradley M. Mitteness ,  Connie Phillips

    IPC分类号： A61K39/395 ,  A61K9/00 ,  A61K39/40

    CPC分类号： A61K39/395 ,  A61K9/0012 ,  A61K9/0063 ,  A61K39/39575 ,  A61K39/40 ,  A61K2039/505 ,  C07K16/1203 ,  C07K16/1207 ,  C07K16/1242 ,  C07K16/1275 ,  C07K16/14 ,  C07K2317/11 ,  C07K2317/23

    摘要： The present disclosure relates to methods and compositions administered to the lymphatic tissue of an animal to prevent or reduce the incidence of disease or disease symptoms caused by one or more disease causing entities. Tonsillar compositions can be administered to the lymphatic tissue in the oral/nasal region of the animal and can include avian antibodies from eggs of hens inoculated with one or more target disease causing entities. Treating the lymphatic tissue in the oral/nasal region with the compositions described herein can reduce or prevent the disease causing entities from adhering to the lymphatic tissue resulting in reduced infection rates. The present disclosure also relates to oral compositions that can be used to reduce or eliminate oral pathogens in animals.

    摘要翻译： 本公开涉及施用于动物的淋巴组织以防止或减少由一种或多种致病实体引起的疾病或疾病症状的发生率的方法和组合物。 扁桃体组合物可以施用于动物的口腔/鼻部区域中的淋巴组织，并且可以包括接种了一种或多种靶向疾病引发实体的母鸡蛋的禽抗体。 用本文所述的组合物治疗口腔/鼻腔中的淋巴组织可以减少或预防引起疾病的实体粘附到淋巴组织，导致感染率降低。 本公开还涉及可用于减少或消除动物体内口腔病原体的口腔组合物。