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1.发明申请公开(公告)号：US20100279306A1
公开(公告)日：2010-11-04
申请号：US12595945
申请日：2008-04-15
CPC分类号： G01N33/6842 , Y10T436/143333
摘要： The present disclosure provides technologies for enriching, detecting, and/or quantifying phosphorylated glycans and/or glycoconjugates (e.g., glycopeptides, glycolipids, proteoglycans, etc.). Specifically, the present disclosure provides methods that utilize immobilized metal affinity chromatography (IMAC) to isolate and/or to characterize phosphorylated glycans. The present disclosure particularly provides methods for distinguishing sulfated and phosphorylated glycans.
摘要翻译： 本公开提供了用于丰富，检测和/或定量磷酸化聚糖和/或糖缀合物（例如糖肽，糖脂，蛋白多糖等）的技术。 具体地，本公开提供利用固定化金属亲和层析（IMAC）分离和/或表征磷酸化聚糖的方法。 本公开特别提供了区分硫酸化和磷酸化聚糖的方法。
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公开(公告)号：US20120264927A1
公开(公告)日：2012-10-18
申请号：US12595934
申请日：2008-04-15
申请人： Ian Christopher Parsons , Lakshmanan Thiruneelakantapillai , Carlos J. Bosques , Brian Edward Collins
发明人： Ian Christopher Parsons , Lakshmanan Thiruneelakantapillai , Carlos J. Bosques , Brian Edward Collins
IPC分类号： C07H1/00
摘要： Methods for labeling glycans that include a step of freeze-drying a labeled glycan preparation. The labeled glycan preparation is maintained in a substantially frozen state for the duration of the freeze-drying process.
摘要翻译： 标记聚糖的方法，其包括冷冻干燥标记的聚糖制剂的步骤。 标记的聚糖制剂在冷冻干燥过程中保持在基本上冷冻的状态。
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公开(公告)号：US20100129843A1
公开(公告)日：2010-05-27
申请号：US12595925
申请日：2008-04-15
申请人： Ian Christopher Parsons , Dorota A. Bulik , Carlos J. Bosques , Lakshmanan Thiruneelakantapillai , Brian Edward Collins
发明人： Ian Christopher Parsons , Dorota A. Bulik , Carlos J. Bosques , Lakshmanan Thiruneelakantapillai , Brian Edward Collins
IPC分类号： C12Q1/34
CPC分类号： C12Q1/34 , F28D15/0266 , G06F1/20 , G06F2200/201 , H01L23/427 , H01L2924/0002 , H05K7/20809 , H01L2924/00
摘要： The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially.
摘要翻译： 本公开提供了分析N-聚糖的结构和/或组成的方法。 这种方法通常涉及用多种除草糖苷酶消化N-聚糖。 在一些实施方案中，N-聚糖同时用多种外切果糖酶消化。 在一些实施方案中，N-多糖被依次用多种除草糖苷酶消化。 在一些实施方案中，根据本公开的方法涉及将已经用多种外切果糖酶同时消化的N-聚糖的切割产物与已经依次用多种除草糖苷酶消化的N-聚糖进行比较。
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公开(公告)号：US08361705B2
公开(公告)日：2013-01-29
申请号：US12595925
申请日：2008-04-15
申请人： Ian Christopher Parsons , Dorota A. Bulik , Carlos J. Bosques , Lakshmanan Thiruneelakantapillai , Brian Edward Collins
发明人： Ian Christopher Parsons , Dorota A. Bulik , Carlos J. Bosques , Lakshmanan Thiruneelakantapillai , Brian Edward Collins
IPC分类号： C12Q1/00
CPC分类号： C12Q1/34 , F28D15/0266 , G06F1/20 , G06F2200/201 , H01L23/427 , H01L2924/0002 , H05K7/20809 , H01L2924/00
摘要： The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially.
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公开(公告)号：US08338088B2
公开(公告)日：2012-12-25
申请号：US12595902
申请日：2008-04-15
申请人： Brian Edward Collins , Carlos J. Bosques , Xiangping Zhu , Dorota A. Bulik , Lakshmanan Thiruneelakantapillai , Ian Christopher Parsons , Zachary Shriver , Rajeev Chillakuru , Ganesh Venkataraman
发明人： Brian Edward Collins , Carlos J. Bosques , Xiangping Zhu , Dorota A. Bulik , Lakshmanan Thiruneelakantapillai , Ian Christopher Parsons , Zachary Shriver , Rajeev Chillakuru , Ganesh Venkataraman
IPC分类号： C12Q1/00
CPC分类号： G01N33/5308 , G01N2400/00
摘要： The present disclosure provides methods for assessing the glycosylation of a target glycoprotein produced by a cell through analysis of cell-surface glycans on the cell. The present disclosure therefore teaches that glycosylation of cell surface proteins can serve as a proxy for glycosylation of other proteins.
摘要翻译： 本公开提供了通过分析细胞上的细胞表面聚糖来评估由细胞产生的目标糖蛋白的糖基化的方法。 因此，本发明公开了细胞表面蛋白的糖基化可以作为其他蛋白质糖基化的代用物。
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公开(公告)号：US20110213137A1
公开(公告)日：2011-09-01
申请号：US12595888
申请日：2008-04-15
申请人： Carlos J. Bosques , Ian Christopher Parsons , Lakshmanan Thiruneelakantapillai , Brian Edward Collins , Hetal Sarvaiya
发明人： Carlos J. Bosques , Ian Christopher Parsons , Lakshmanan Thiruneelakantapillai , Brian Edward Collins , Hetal Sarvaiya
CPC分类号： C07B59/005 , Y10T436/143333
摘要： The present disclosure provides methods of making isotopically-labeled glycans or a mixture of glycans (e.g., a glycan preparation) via reductive amination. Such methods are useful, for example, in the determination of the relative amounts of a glycan species present in two or more glycoprotein preparations using mass spectroscopy. In one aspect, the present disclosure provides a method of making an isotopically-labeled glycan by a glycan with an amine and an isotopically-labeled reducing agent to provide an isotopically-labeled aminated glycan. In certain embodiments, the amine is an isotopically-labeled amine.
摘要翻译： 本公开提供通过还原胺化制备同位素标记的聚糖或聚糖混合物（例如聚糖制备物）的方法。 这样的方法可用于例如使用质谱测定存在于两种或更多种糖蛋白制剂中的聚糖物质的相对量。 一方面，本公开提供了通过聚糖与胺和同位素标记的还原剂制备同位素标记的聚糖以提供同位素标记的胺化聚糖的方法。 在某些实施方案中，胺是同位素标记的胺。
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公开(公告)号：US20100196940A1
公开(公告)日：2010-08-05
申请号：US12595902
申请日：2008-04-15
申请人： Brian Edward Collins , Carlos J. Bosques , Xiangping Zhu , Dorota A. Bulik , Lakshmanan Thiruneelakantapillai , Ian Christopher Parsons , Zachary Shriver , Rajeev Chillakuru , Ganesh Venkataraman
发明人： Brian Edward Collins , Carlos J. Bosques , Xiangping Zhu , Dorota A. Bulik , Lakshmanan Thiruneelakantapillai , Ian Christopher Parsons , Zachary Shriver , Rajeev Chillakuru , Ganesh Venkataraman
CPC分类号： G01N33/5308 , G01N2400/00
摘要： The present disclosure provides methods for assessing the glycosylation of a target glycoprotein produced by a cell through analysis of cell-surface glycans on the cell. The present disclosure therefore teaches that glycosylation of cell surface proteins can serve as a proxy for glycosylation of other proteins.
摘要翻译： 本公开提供了通过分析细胞上的细胞表面聚糖来评估由细胞产生的目标糖蛋白的糖基化的方法。 因此，本发明公开了细胞表面蛋白的糖基化可以作为其他蛋白质糖基化的代用物。
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公开(公告)号：US20100113294A1
公开(公告)日：2010-05-06
申请号：US12596171
申请日：2008-04-15
申请人： Ganesh Venkataraman , Brian Edward Collins , Carlos J. Bosques , Lakshmanan Thiruneelakantapillai , Dorota A. Bulik , Ian Christopher Parsons , Zachary Shriver , Rajeev Chillakuru
发明人： Ganesh Venkataraman , Brian Edward Collins , Carlos J. Bosques , Lakshmanan Thiruneelakantapillai , Dorota A. Bulik , Ian Christopher Parsons , Zachary Shriver , Rajeev Chillakuru
CPC分类号： C12P21/005 , G16B50/00
摘要： The invention provides methods, databases and systems for making glycoprotein products having defined properties.
摘要翻译： 本发明提供了制备具有确定特性的糖蛋白产物的方法，数据库和系统。
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公开(公告)号：US20130123126A1
公开(公告)日：2013-05-16
申请号：US13637972
申请日：2011-04-07
申请人： Brian Edward Collins , Jay Duffner , Victor Farutin , Naveen Bhatnagar , Lakshmanan Thiruneelakantapillai , Carlos J. Bosques , Ganesh Kaundinya
发明人： Brian Edward Collins , Jay Duffner , Victor Farutin , Naveen Bhatnagar , Lakshmanan Thiruneelakantapillai , Carlos J. Bosques , Ganesh Kaundinya
CPC分类号： C12Q1/04 , C12P21/005 , C12Q1/6809 , G06F19/32
摘要： A method of making a glycoprotein having a selected glycostructure.
摘要翻译： 制备具有选定糖结构的糖蛋白的方法。
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公开(公告)号：US20120277165A1
公开(公告)日：2012-11-01
申请号：US13375577
申请日：2010-06-04
IPC分类号： C12P21/00 , C12N5/071 , C12M3/00 , A61K38/14 , G01N27/62 , G01N30/02 , G01N24/08 , C12Q3/00 , C12P21/08 , C12Q1/02
CPC分类号： C12P21/005
摘要： The present invention provides methods and materials useful for monitoring and regulating the glycosylation of glycoproteins that are recombinantly produced from cells. In particular, methods are provided for monitoring and regulating levels of cellular indicators which affect the level of fucosylation produced by cells.
摘要翻译： 本发明提供了用于监测和调节从细胞重组产生的糖蛋白糖基化的方法和材料。 特别地，提供了用于监测和调节影响细胞产生的岩藻糖基化水平的细胞指标水平的方法。
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