公开(公告)号：US20190009270A1

公开(公告)日：2019-01-10

申请号：US16130498

申请日：2018-09-13

申请人： Cedars-Sinai Medical Center ,  Emulate, Inc.

发明人： Dan GAZIT ,  Gadi PELLED ,  Zulma GAZIT ,  Dmitriy SHEYN ,  Christopher David Hinojosa ,  Norman Wen ,  Geraldine Hamilton

IPC分类号： B01L3/00 ,  G01T1/164 ,  G01T1/29 ,  A61K51/02 ,  A61K49/00 ,  C12N5/074 ,  C12N5/071 ,  C12N5/0775

摘要： Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.

公开(公告)号：US20190018000A1

公开(公告)日：2019-01-17

申请号：US16069361

申请日：2017-01-12

申请人： Cedars-Sinai Medical Center

发明人： Dan GAZIT ,  Gadi PELLED ,  Zulma GAZIT ,  Dmitriy SHEYN

IPC分类号： G01N33/50 ,  C12N5/00

摘要： Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.

公开(公告)号：US20200093961A1

公开(公告)日：2020-03-26

申请号：US15739555

申请日：2016-06-22

申请人： Cedars-Sinai Medical Center

发明人： Zulma Gazit ,  Dmitriy SHEYN ,  Gadi PELLED ,  Dan GAZIT

IPC分类号： A61L27/38 ,  A61L27/52 ,  C12N5/077 ,  A61L27/22

摘要： Described herein are compositions and methods related to derivation of human notochordal cells differentiated from induced pluripotent stem cells (iPSCs). The inventors have developed a two-step process for generating these iPSC-derived notochordal cells (iNCs), which can provide a renewable source of therapeutic material for use in degenerative disc disease (DDD). As iNCs are capable of reversing DDD and supporting regeneration of invertebral disc (IVD) tissue based on the understanding that NC cells maintain homeostasis and repair of other IVD cell types such as nuclear pulposus (NP).